CAJ | 학술논문

目的检测缺氧标记物碳酸酐酶Ⅸ(CA-Ⅸ)在128例直肠癌不同部位组织中的表达,探讨它与直肠癌肝转移及与血管内皮生长因子(VEGF)的关系,了解其对直肠癌细胞体外生长能力的影响.方法以CA-Ⅸ和VEGF为研究对象,应用蛋白免疫印迹方法检测两者在正常直肠组织,直肠腺瘤,直肠癌患者原发癌灶、系膜组织、肝转移组织中的差异性表达,判断它们作为直肠癌和直肠癌转移相关因子的意义.观察CA广谱抑制剂乙酰唑胺对体外直肠癌细胞生长能力的影响.结果有肝转移的直肠癌患者原发灶中VEGF和CA-Ⅸ阳性率均为83.3%,高于正常直肠组织(16.7%和0%)、直肠腺瘤(16.7%和36.7%)和无肝转移直肠原发癌灶(72.1%和65.4%)(P<0.01),肝转移直肠癌患者系膜组织中VEGF和CA-Ⅸ阳性率也高于无肝转移患者,但差异无统计学意义(P>0.05),VEGF与CA-Ⅸ表达和肝转移发生无明显相关.乙酰唑胺能提高不同浓度5-氟尿嘧啶(5-Fu)体外对直肠癌细胞的杀伤能力(P<0.05).结论 CA-Ⅸ在直肠癌组织中高表达,对直肠癌细胞生长增殖有促进作用,但不宜作为诊断肝转移的独立因子.
목적 검측결양표기물탄산항매Ⅸ(CA-Ⅸ)재128례직장암불동부위조직중적표체,탐토타여직장암간전이급여혈관내피생장인자(VEGF)적관계,료해기대직장암세포체외생장능력적영향.방법 이CA-Ⅸ화VEGF위연구대상,응용단백면역인적방법검측량자재정상직장조직,직장선류,직장암환자원발암조、계막조직、간전이조직중적차이성표체,판단타문작위직장암화직장암전이상관인자적의의.관찰CA엄보억제제을선서알대체외직장암세포생장능력적영향.결과유간전이적직장암환자원발조중VEGF화CA-Ⅸ양성솔균위83.3%,고우정상직장조직(16.7%화0%)、직장선류(16.7%화36.7%)화무간전이직장원발암조(72.1%화65.4%)(P<0.01),간전이직장암환자계막조직중VEGF화CA-Ⅸ양성솔야고우무간전이환자,단차이무통계학의의(P>0.05),VEGF여CA-Ⅸ표체화간전이발생무명현상관.을선서알능제고불동농도5-불뇨밀정(5-Fu)체외대직장암세포적살상능력(P<0.05).결론 CA-Ⅸ재직장암조직중고표체,대직장암세포생장증식유촉진작용,단불의작위진단간전이적독립인자.
Objective To explore the differential expression of carbonic anhydrases-Ⅸ ( CA-Ⅸ )in 128 patients with rectal cancer,and study its association with vascular endothelial growth factor (VEGF)and hepatic metastasis of rectal cancer.Methods Western blotting was used to examine the expression of CA-Ⅸ and VEGF in different tissues of 128 patients with rectal cancer and 30 cases of normal rectal mucosa and rectal adenoma,and the influence of anthdrases's inhibitor ( acetazolamide ) on the rectal cancer cell growth was studied.Results The positive expression rate of both CA-Ⅸ and VEGF in carcinoma tissues of patients with hepatic metastasis was 83.3%,which was higher than that in normal rectal tissue ( 16.7%,0% ) and rectal adenoma ( 16.7%,36.7% ) and carcinoma tissues of patients without hepatic metastasis (72.1%,65.4% ,P ＜0.01 ).The expression of VEGF and CA-Ⅸ in mesorectal tissues of the patients with hepatic metastasis was not higher than that of the patients without hepatic metastasis.There was a negative association between the expression of CA-Ⅸ with VEGF and hepatic metastasis of the patients with rectal cancer.Acetazolamide obviously enhanced the chemotaxymotor ability of fluorouracil to increase the apoptosis of recetal cancer cells.Conclusion The CA-Ⅸ is highly expressed in rectal cancer tissues and can promote the growth and proliferation of rectal cancer cells,but it cann't be used as an independent index to make the diagnosis of hepatic metastasis in rectal cancer patients.