CAJ | 학술논문

目的利用癫痫动物模型评价聚氰基丙烯酸正丁酯纳米微粒(DPH-PBCA-NPs)和Tween-80修饰的DPH-PBCA-NPs抗癫痫疗效,探讨纳米载药系统的优越性.方法构建氯化锂-匹罗卡品急性癫痫大鼠模型,按照完全随机数字表法分为5组,Ⅰ组注射Tween-80修饰的DPH-PBCA-NPs,Ⅱ组注射DPH-PBCA-NPs,Ⅲ组注射苯妥英钠水溶液,Ⅳ组注射PBCA-NPs,Ⅴ组注射生理盐水.利用视频脑电监测仪,观察EEG动态变化过程,同时观察致痫大鼠的行为学改变.结果成功构建氯化锂-匹罗卡品急性癫痫大鼠模型,致痫大鼠在行为和EEG上均表现出了癫痫持续状态.Ⅰ组治疗有效率为91.67%,Ⅱ组有效率为54.55%,Ⅲ组有效率为50%,Ⅳ、Ⅴ组有效率为0%,差异有统计学意义(P＜0.05).结论 DPH-PBCA-NPs和Tween-80修饰的DPH-PBCA-NPs能够明显改善致痫大鼠的行为学表现和EEG结果,具有抗癫痫作用.纳米载药系统能协助药物更迅速、更有效地发挥治疗作用.
목적 이용전간동물모형평개취청기병희산정정지납미미립(DPH-PBCA-NPs)화Tween-80수식적DPH-PBCA-NPs항전간료효,탐토납미재약계통적우월성.방법 구건록화리-필라잡품급성전간대서모형,안조완전수궤수자표법분위5조,Ⅰ조주사Tween-80수식적DPH-PBCA-NPs,Ⅱ조주사DPH-PBCA-NPs,Ⅲ조주사분타영납수용액,Ⅳ조주사PBCA-NPs,Ⅴ조주사생리염수.이용시빈뇌전감측의,관찰EEG동태변화과정,동시관찰치간대서적행위학개변.결과 성공구건록화리-필라잡품급성전간대서모형,치간대서재행위화EEG상균표현출료전간지속상태.Ⅰ조치료유효솔위91.67%,Ⅱ조유효솔위54.55%,Ⅲ조유효솔위50%,Ⅳ、Ⅴ조유효솔위0%,차이유통계학의의(P＜0.05).결론 DPH-PBCA-NPs화Tween-80수식적DPH-PBCA-NPs능구명현개선치간대서적행위학표현화EEG결과,구유항전간작용.납미재약계통능협조약물경신속、경유효지발휘치료작용.
Objective To evaluate the effects of diphenylhydantoin sodiumpolybutylcyanoacrylate nanoparticles (DPH-PBCA-NPs) and DPH-PBCA-NPs modified with Tween-80on rat models of epilepsy, and investigate the advantage of nanoparticle as the drug delivery system.Methods The rat models of acute epilepsy induced by lithium pilocarpine were established and randomly divided into 5 groups: group Ⅰ (performing injection of DPH-PBCA-NPs modified with Tween-80), group Ⅱ (performing injection of DPH-PBCA-NPs), group Ⅲ (performing injection of diphenylhydantoin sodium), group Ⅳ (performing injection of PBCA-NPs) and group Ⅴ (performing injection of physiological saline). The changes of electroencephalogram (EEG) manifestations of these rats were observed by using video-EEG monitoring; and their behavioral changes were noted too.Results The lithium pilocarpine induced rat models of acute epilepsy were successfully established and their status epilepticus were confirmed by EEG and their behaviors. The effective rate of DPH-PBCA-NPs modified with Tween-80 and DPH-PBCA-NPs was 91.67% and 54.55%, respectively;the effective rate of rats in group Ⅲ was 50%, and the effective rate of rats in group Ⅳ and Ⅴ was 0%;DPH-PBCA-NPs modified with Tween-80 enjoyed a better effect than DPH-PBCA-NPs and DPH (P＜0.05). Conclusion DPH-PBCA-NPs and DPH-PBCA-NPs modified with Tween-80 can be used to improve the behaviors of rats with acute epilepsy and modify the results of EEG of these rats.Nanoparticles as drug delivery system can help the drugs having their effects much quickly and effectively.