目的 评价转GmDREB1基因抗旱小麦T349的免疫毒性作用.方法 将250只6～8周龄、体重为18～22 g的SPF级雌性BALB/c小鼠按体重分层随机分为5个大组,每大组50只,每大组再按体重分层随机分为5组,每组10只,分别为阴性对照组、普通小麦组、亲本小麦组、转基因小麦组和环磷酰胺阳性对照组.阴性对照组和环磷酰胺阳性对照组以AIN-93G饲料喂养,普通小麦组、亲本小麦组和转基因小麦组以掺入相应小麦的饲料喂养.饲喂30 d后,检测小鼠的各项指标,包括体重、脏器系数、外周血淋巴细胞表型、血清细胞因子水平、血清免疫球蛋白水平、溶血空斑数、血清半数溶血值( HCso)、脾淋巴细胞增殖能力、迟发型超敏反应和吞噬细胞功能.结果 阴性对照组、普通小麦组、亲本小麦组、转基因小麦组、阳性对照组小鼠饲喂30 d后体重分别为(21.0±0.3)、(20.4±0.7)、(21.1±1.0)、(21.1±1.0)、(19.4±1.0)g (F=7.47,P＜0.01)；脾脏器系数分别为(0.407±0.047)％、(0.390±0.028)％、(0.402±0.042)％、(0.421±0.041)％、(0.304±0.048)％(F=12.41,P＜0.01)；胸腺脏器系数分别为(0.234±0.032)％、(0.246±0.028)％、(0.249±0.040)％、(0.234±0.034)％、(0.185±0.039)％ (F =5.58,P＜0.01)；小鼠外周血T淋巴细胞百分比分别为(70.43±4.44)％、(68.33±5.37)％、(73.04±2.68)％、(74.42±2.86)％、(90.42±1.66)％(F=57.51,P＜0.01)；B淋巴细胞百分比分别为(13.89±3.19)％、(15.34 ±4.84)％、(13.06±4.22)％、(12.93±2.36)％、(3.01±0.96)％(F=12.79,P＜0.01)；Th细胞百分比分别为(55.87±3.80)％、(55.24±4.60)％、(57.92±3.70)％、(59.57±2.54)％、(77.37±2.31)％(F=68.58,P＜0.01)；辅助性T细胞(Th)/抑制性T细胞(Ts)比值分别为4.16±0.29、4.73±0.96、4.19±0.78、4.52±0.40、6.34±0.73(F=17.57,P＜0.01)；小鼠血清IgG水平分别为(1046.38±210.67)、(1065.49±297.22)、(1517.73±299.52)、(1576.67±241.92)、(1155.88±167.05) μg/ml (F=10.53,P＜0.01)；小鼠血清IgM水平分别为(333.83±18.97)、(327.73±27.72)、(367.47±27.18)、(363.42±46.14)、(278.71±24.42) μg/ml (F=12.11,P＜0.01)；小鼠血清IgA水平分别为(51.69±10.10)、(42.40±8.35)、(32.11±4.22)、(37.12±4.90)、(41.45±8.89) μg/ml (F=8.25,P＜0.01)；小鼠溶血空斑数分别为(29.2±14.6)、(28.0±20.0)、(34.8±30.9)、(33.2±25.1)、(4.8±5.3)个/106个脾细胞(F=3.33,P＜0.05)；HC50值分别为82.3±6.5、79.7±4.6、75.8±4.1、74.9±3.6、70.8±2.1 (F=9.99,P＜0.01)；脂多糖诱导的脾细胞增殖能力分别为0.21±0.10、0.21±0.14、0.26±0.12、0.25 ±0.14、0.07±0.06(F=4.18,P＜0.05).结论 转GmDREB1基因抗旱小麦T349与亲本小麦对小鼠的免疫器官及免疫功能的影响具有实质等同性,无免疫毒性作用.
目的 評價轉GmDREB1基因抗旱小麥T349的免疫毒性作用.方法 將250隻6～8週齡、體重為18～22 g的SPF級雌性BALB/c小鼠按體重分層隨機分為5箇大組,每大組50隻,每大組再按體重分層隨機分為5組,每組10隻,分彆為陰性對照組、普通小麥組、親本小麥組、轉基因小麥組和環燐酰胺暘性對照組.陰性對照組和環燐酰胺暘性對照組以AIN-93G飼料餵養,普通小麥組、親本小麥組和轉基因小麥組以摻入相應小麥的飼料餵養.飼餵30 d後,檢測小鼠的各項指標,包括體重、髒器繫數、外週血淋巴細胞錶型、血清細胞因子水平、血清免疫毬蛋白水平、溶血空斑數、血清半數溶血值( HCso)、脾淋巴細胞增殖能力、遲髮型超敏反應和吞噬細胞功能.結果 陰性對照組、普通小麥組、親本小麥組、轉基因小麥組、暘性對照組小鼠飼餵30 d後體重分彆為(21.0±0.3)、(20.4±0.7)、(21.1±1.0)、(21.1±1.0)、(19.4±1.0)g (F=7.47,P＜0.01)；脾髒器繫數分彆為(0.407±0.047)％、(0.390±0.028)％、(0.402±0.042)％、(0.421±0.041)％、(0.304±0.048)％(F=12.41,P＜0.01)；胸腺髒器繫數分彆為(0.234±0.032)％、(0.246±0.028)％、(0.249±0.040)％、(0.234±0.034)％、(0.185±0.039)％ (F =5.58,P＜0.01)；小鼠外週血T淋巴細胞百分比分彆為(70.43±4.44)％、(68.33±5.37)％、(73.04±2.68)％、(74.42±2.86)％、(90.42±1.66)％(F=57.51,P＜0.01)；B淋巴細胞百分比分彆為(13.89±3.19)％、(15.34 ±4.84)％、(13.06±4.22)％、(12.93±2.36)％、(3.01±0.96)％(F=12.79,P＜0.01)；Th細胞百分比分彆為(55.87±3.80)％、(55.24±4.60)％、(57.92±3.70)％、(59.57±2.54)％、(77.37±2.31)％(F=68.58,P＜0.01)；輔助性T細胞(Th)/抑製性T細胞(Ts)比值分彆為4.16±0.29、4.73±0.96、4.19±0.78、4.52±0.40、6.34±0.73(F=17.57,P＜0.01)；小鼠血清IgG水平分彆為(1046.38±210.67)、(1065.49±297.22)、(1517.73±299.52)、(1576.67±241.92)、(1155.88±167.05) μg/ml (F=10.53,P＜0.01)；小鼠血清IgM水平分彆為(333.83±18.97)、(327.73±27.72)、(367.47±27.18)、(363.42±46.14)、(278.71±24.42) μg/ml (F=12.11,P＜0.01)；小鼠血清IgA水平分彆為(51.69±10.10)、(42.40±8.35)、(32.11±4.22)、(37.12±4.90)、(41.45±8.89) μg/ml (F=8.25,P＜0.01)；小鼠溶血空斑數分彆為(29.2±14.6)、(28.0±20.0)、(34.8±30.9)、(33.2±25.1)、(4.8±5.3)箇/106箇脾細胞(F=3.33,P＜0.05)；HC50值分彆為82.3±6.5、79.7±4.6、75.8±4.1、74.9±3.6、70.8±2.1 (F=9.99,P＜0.01)；脂多糖誘導的脾細胞增殖能力分彆為0.21±0.10、0.21±0.14、0.26±0.12、0.25 ±0.14、0.07±0.06(F=4.18,P＜0.05).結論 轉GmDREB1基因抗旱小麥T349與親本小麥對小鼠的免疫器官及免疫功能的影響具有實質等同性,無免疫毒性作用.
목적 평개전GmDREB1기인항한소맥T349적면역독성작용.방법 장250지6～8주령、체중위18～22 g적SPF급자성BALB/c소서안체중분층수궤분위5개대조,매대조50지,매대조재안체중분층수궤분위5조,매조10지,분별위음성대조조、보통소맥조、친본소맥조、전기인소맥조화배린선알양성대조조.음성대조조화배린선알양성대조조이AIN-93G사료위양,보통소맥조、친본소맥조화전기인소맥조이참입상응소맥적사료위양.사위30 d후,검측소서적각항지표,포괄체중、장기계수、외주혈림파세포표형、혈청세포인자수평、혈청면역구단백수평、용혈공반수、혈청반수용혈치( HCso)、비림파세포증식능력、지발형초민반응화탄서세포공능.결과 음성대조조、보통소맥조、친본소맥조、전기인소맥조、양성대조조소서사위30 d후체중분별위(21.0±0.3)、(20.4±0.7)、(21.1±1.0)、(21.1±1.0)、(19.4±1.0)g (F=7.47,P＜0.01)；비장기계수분별위(0.407±0.047)％、(0.390±0.028)％、(0.402±0.042)％、(0.421±0.041)％、(0.304±0.048)％(F=12.41,P＜0.01)；흉선장기계수분별위(0.234±0.032)％、(0.246±0.028)％、(0.249±0.040)％、(0.234±0.034)％、(0.185±0.039)％ (F =5.58,P＜0.01)；소서외주혈T림파세포백분비분별위(70.43±4.44)％、(68.33±5.37)％、(73.04±2.68)％、(74.42±2.86)％、(90.42±1.66)％(F=57.51,P＜0.01)；B림파세포백분비분별위(13.89±3.19)％、(15.34 ±4.84)％、(13.06±4.22)％、(12.93±2.36)％、(3.01±0.96)％(F=12.79,P＜0.01)；Th세포백분비분별위(55.87±3.80)％、(55.24±4.60)％、(57.92±3.70)％、(59.57±2.54)％、(77.37±2.31)％(F=68.58,P＜0.01)；보조성T세포(Th)/억제성T세포(Ts)비치분별위4.16±0.29、4.73±0.96、4.19±0.78、4.52±0.40、6.34±0.73(F=17.57,P＜0.01)；소서혈청IgG수평분별위(1046.38±210.67)、(1065.49±297.22)、(1517.73±299.52)、(1576.67±241.92)、(1155.88±167.05) μg/ml (F=10.53,P＜0.01)；소서혈청IgM수평분별위(333.83±18.97)、(327.73±27.72)、(367.47±27.18)、(363.42±46.14)、(278.71±24.42) μg/ml (F=12.11,P＜0.01)；소서혈청IgA수평분별위(51.69±10.10)、(42.40±8.35)、(32.11±4.22)、(37.12±4.90)、(41.45±8.89) μg/ml (F=8.25,P＜0.01)；소서용혈공반수분별위(29.2±14.6)、(28.0±20.0)、(34.8±30.9)、(33.2±25.1)、(4.8±5.3)개/106개비세포(F=3.33,P＜0.05)；HC50치분별위82.3±6.5、79.7±4.6、75.8±4.1、74.9±3.6、70.8±2.1 (F=9.99,P＜0.01)；지다당유도적비세포증식능력분별위0.21±0.10、0.21±0.14、0.26±0.12、0.25 ±0.14、0.07±0.06(F=4.18,P＜0.05).결론 전GmDREB1기인항한소맥T349여친본소맥대소서적면역기관급면역공능적영향구유실질등동성,무면역독성작용.
Objective To assess the immunotoxicologic effects of genetically modified droughtresistant wheat T349 with GmDREB1 gene.Methods A total of 250 female BALB/c mice(6 -8 week-old,weight 18-22 g) were divided into five large groups(50 mice for each large group) by body weight randomly.In each large group,the mice were divided into five groups( 10 mice for each group) by body weight randomly,which were set as negative control group,common wheat group,parental wheat group,genetically modified wheat group and cyclophosphamide positive control pup,respectively.Mice in negative control and positive control group were fed with feedstuff AIN-93G,mice in common wheat group,nongenetically modified parental wheat group and genetically modified wheat group were fed with feedstuffs added corresponding wheat ( proportion up to 76％ ) for 30 days,then body weight,organ coefficient of spleen and thymus,peripheral blood lymphocytes phenotyping,serum cytokine,serum immunoglobulin,antibody plaque-forming cell ( PFC),serum 50％ hemolytic value ( HC50 ),mitogen-indueed splenocyte proliferation,delayed-type hypersensitivity (DTH) reaction and phagocytic activities of phagocytes were detected respectively.Results After 30 days raise,among negative control group,common wheat group,nongenetically modified parental wheat group,genetically modified wheat group and cyclophosphamide positive control group,mice body weight were (21.0 ±0.3),(20.4 ±0.7),(21.1 ± 1.0),(21.1 ± 1.0),(19.4±1.0) g,respectively(F=7.47,P＜0.01); organ coefficient of spleen were (0.407 ±0.047)％,(0.390 ±0.028)％,(0.402±0.042)％,(0.421 ±0.041)％,(0.304±0.048)％,respectively (F=12.41,P ＜0.01 ) ; organ coefficient of thymus were (0.234 ±0.032)％,(0.246 ±0.028)％,(0.249 ±0.040)％,(0.234±0.034)％,(0.185±0.039)％,respectively (F=5.58,P＜0.01);the percentage of T cell in peripheral blood were ( 70.43 ± 4.44 ) ％,( 68.33 ± 5.37 ) ％,( 73.04 ± 2.68 ) ％,(74.42 ±2.86)％,(90.42 ± 1.66)％,respectively (F =57.51,P ＜0.01); the percentage of B cell were (13.89±3.19)％,(15.34 ±4.84)％,(13.06±4.22)％,(12.93 ±2.36)％,(3.01 ±0.96)％,respectively ( F =12.79,P ＜ 0.01 ) ; the percentage of Th cell were ( 55.87 ± 3.80 ) ％,( 55.24 ±4.60)％,(57.92 ±3.70)％,(59.57 ±2.54)％,(77.37 ±2.31)％,respectively (F=68.58,P＜0.01) ; the Th/Ts ratio were 4.16 ± 0.29,4.73 ± 0.96,4.19 ± 0.78,4.52 ± 0.40,6.34 ± 0.73,respectively ( F =17.57,P ＜ 0.01 ) ; the serum IgG were ( 1046.38 ± 210.67 ),( 1065.49 ± 297.22 ),( 1517.73 ± 299.52 ),( 1576.67 ± 241.92),( 1155.88 ± 167.05 ) μg/ml,respectively ( F =10.53,P ＜0.01 ) ; the serum IgM were ( 333.83 ± 18.97 ),( 327.73 ± 27.72 ),( 367.47 ± 27.18 ),( 363.42 ±46.14),(278.71 ±24.42) μg/ml,respectively (F=12.11,P＜0.01) ; the serum IgA were (51.69 ±10.10),(42.40 ±8.35),(32.11 ±4.22),(37.12 ±4.90),(41.45 ±8.89) μg/ml,respectively (F=8.25,P＜0.01) ; the PFC were (29.2±14.6),(28.0±20.0),(34.8 ±30.9),(33.2±25.1),(4.8±5.3 ) per 106 splenocyte,respectively ( F =3.33,P ＜ 0.05 ) ; the HC50 were 82.3 ± 6.5,79.7 ± 4.6,75.8 ±4.1,74.9 ± 3.6,70.8 ± 2.1,respectively ( F =9.99,P ＜ 0.01 ) ; the LPS-induced splenocyte proliferation were 0.21 ± 0.10,0.21 ± 0.14,0.26 ± 0.12,0.25 ± 0.14,0.07 ± 0.06,respectively ( F =4.18,P ＜ 0.05 ).Conclusion The genetically modified drought-resistant wheat T349 was substantially equivalent to parental wheat in the effects on immune organs and immunologic functions of mice,and it didn't show immunotoxicity.
