CAJ | 학술논문

在蔷薇科、茄科和玄参科配子体自交不亲和中,编码花柱的S RNase控制花柱的自交不亲和性.在前两科植物中,自交不亲和(S)位点定位于着丝粒的附近,但在玄参科植物金鱼草(Antirrhinum)中自交不亲和位点至今未知.为了确定它在染色体上的位置和基因组结构,以基因型S2S5金鱼草根尖为材料,进行染色体的制备观察,利用地高辛标记的S2 RNase和含有其全长的BAC克隆(S2 BAC)为探针进行荧光染色体原位杂交(FISH),发现S2RNase杂交信号位于染色体的着丝粒附近,而S2 BAC的杂交信号位于每条染色体的着丝粒的周边区,呈对称的4个,表明金鱼草S位点位于着丝粒的周边区.对S2BAC预测基因的分析表明,发现一个金鱼草新的反转座子(RIS1).结果显示,金鱼草S位点位于染色体着丝粒的周边区,富含转座子和反转座子,和其他两类配子体自交不亲和的位置类似,预示它们的共同起源和具有抑制重组的功能.
재장미과、가과화현삼과배자체자교불친화중,편마화주적S RNase공제화주적자교불친화성.재전량과식물중,자교불친화(S)위점정위우착사립적부근,단재현삼과식물금어초(Antirrhinum)중자교불친화위점지금미지.위료학정타재염색체상적위치화기인조결구,이기인형S2S5금어초근첨위재료,진행염색체적제비관찰,이용지고신표기적S2 RNase화함유기전장적BAC극륭(S2 BAC)위탐침진행형광염색체원위잡교(FISH),발현S2RNase잡교신호위우염색체적착사립부근,이S2 BAC적잡교신호위우매조염색체적착사립적주변구,정대칭적4개,표명금어초S위점위우착사립적주변구.대S2BAC예측기인적분석표명,발현일개금어초신적반전좌자(RIS1).결과현시,금어초S위점위우염색체착사립적주변구,부함전좌자화반전좌자,화기타량류배자체자교불친화적위치유사,예시타문적공동기원화구유억제중조적공능.
The self-incompatibility (S) loci from the Solanaceae, Rosaceae and Scrophulariaceae encode a class of ribonucleases, known as S RNases, which have been shown to control the pistil expression of self-incompatible reaction. In the former two families, the S loci have been shown to be located near centromere.However, the chromosomal location of the S locus in Antirrhinum, a species of the Scrophulariaceae, is not known. To determine its chromosomal location and genomic organization, an S2 RNase gene and its corresponding 63 kb BAC clone were separately used for fluorescence in situ hybridization (FISH) of mitotic metaphase chromosomes of a self-incompatible Antirrhinum line of S2Ss. The results showed that the S2 RNase detected a doublet signal near the centromere of the smallest chromosome (2n = 16). Two separate doublet signals of the tested BAC sequence were shown on both sides of the centromeres of all eight pairs of the chromosomes, suggesting that the Antirrhinum S locus is located in a pericentromeric region. Furthermore, a retrotransposon, named RIS1 (retrotransposon in the S locus), which has not been identified yet in Antirrhinum, was found next to S2 RNase. Taken together, the centromeric location of the S locus from the three S-RNase-based self-incompatible families provides a further support on a common origin of their evolution as well as suppressed recombination.