CAJ | 학술논문

采用聚丙烯酰胺凝胶电泳、酶联染色、光密度扫描、分光光度法以及电镜酶细胞化学等方法，对12例生育男性(生育组)和14例不育男性(不育组)精子LDHx进行了研究。结果显示LDHx电泳区带位于LDH3和LDH4之间，生育组精子LDHx绝对活性和相对活性均高于不育组(P＜0.05)。相关分析表明精子密度与LDHx绝对活性和相对活性均具有相关性，在生育组呈正相关(r=0.8和0.75，P＜0.05)，在不育组呈负相关(r=-0.76和-0.78，P＜0.05)。LDHx酶细胞化学定位分析显示LDHx酶反应颗粒主要分布于精子型线粒体(STM)和胞质内，少量分布于顶体及质膜表面。生育组精子各部位酶反应颗粒多于不育组，且不育组精子多有畸形并伴有超微结构改变。上述研究分析提示，精子LDHx活性测定与定位分析可作为检查不育症精子质量的可靠指标，为男性不育症的临床诊断提供实验依据。
채용취병희선알응효전영、매련염색、광밀도소묘、분광광도법이급전경매세포화학등방법，대12례생육남성(생육조)화14례불육남성(불육조)정자LDHx진행료연구。결과현시LDHx전영구대위우LDH3화LDH4지간，생육조정자LDHx절대활성화상대활성균고우불육조(P＜0.05)。상관분석표명정자밀도여LDHx절대활성화상대활성균구유상관성，재생육조정정상관(r=0.8화0.75，P＜0.05)，재불육조정부상관(r=-0.76화-0.78，P＜0.05)。LDHx매세포화학정위분석현시LDHx매반응과립주요분포우정자형선립체(STM)화포질내，소량분포우정체급질막표면。생육조정자각부위매반응과립다우불육조，차불육조정자다유기형병반유초미결구개변。상술연구분석제시，정자LDHx활성측정여정위분석가작위검사불육증정자질량적가고지표，위남성불육증적림상진단제공실험의거。
The methods of polyacrylamide gel electrophoresis (PAGE),enzyme-linked staining, desitometric scanning, spectrophotometry and electron microscopic enzyme cytochemistry were used to stduy LDHx activity of sperm obtained from 12 fertile men and 14 infertile men. The results showed that the “X-band” appeared in the major area of LDH between LDH3 and LDH4 on lactate dehydrogenase isoenzyme electrophoretic pattern. The absolute and relative activity of spermatozoon LDHx in fertile group were higher than that of infertile group (P＜0.05). In fertile group, the absolute and relative activity of spermatozoon LDHx were related positively to sperm concentration (r=0.8 and 0.75，respectively, P＜0.05). But in infertile group, this relationship showed inverse correlation (r=-0.76 and -0.78，respectively, P＜0.05). Electron microscopic cytochemistry proved that the enzymic particles of LDHx were mainly localizated in the matrix of sperm-type-mitochondria, acrosome, cytoplasm and plasma membrane and some escaped out of membrane. The number of enzymic particles of LDHx in fertile spermatozoon was apparently larger than those in infertile spermatozoon. Moreover, spermatozoon from infertile men mostly showed deformity and a change of spermatic ultrastructure. These results suggested that the examining of LDHx activity and the study of the distribution of LDHx may be used as a reliable marker for testing the quality of spermatozoa and diagnosing the male sterility.