CAJ | 학술논문

CoQ10因其具有多种生理生化功能而不仅用于药物也用作食品添加剂.本研究采用全因析中心复合设计及响应面法对茄尼醇、对羟基苯甲酸及甲硫氨酸三种CoQ10前体物的添加量进行了优化,以达到沼泽红假单胞菌J001最大量地生产CoQ10的目的.结果表明:经过对所得模型进行响应曲面法分析,当茄尼醇、对羟基苯甲酸及甲硫氨酸最佳添加量分别为124.8mg1-1,267.7mg1-1,130.2mg1-1时,得最大CoQ10产量预测值40.6[(mgCoQ10)(g干细胞)-1].对上述最佳组合进行试验验证得40.5±0.2[(mg CoQ10)(g干细胞)-1],很接近预测值,比对照(未加三种CoQ10前体物)CoQ10产量提高了109.8%.
CoQ10인기구유다충생리생화공능이불부용우약물야용작식품첨가제.본연구채용전인석중심복합설계급향응면법대가니순、대간기분갑산급갑류안산삼충CoQ10전체물적첨가량진행료우화,이체도소택홍가단포균J001최대량지생산CoQ10적목적.결과표명:경과대소득모형진행향응곡면법분석,당가니순、대간기분갑산급갑류안산최가첨가량분별위124.8mg1-1,267.7mg1-1,130.2mg1-1시,득최대CoQ10산량예측치40.6[(mgCoQ10)(g간세포)-1].대상술최가조합진행시험험증득40.5±0.2[(mg CoQ10)(g간세포)-1],흔접근예측치,비대조(미가삼충CoQ10전체물)CoQ10산량제고료109.8%.
CoQ10 has been used not only as a medicine but also as food supplements because of its various physiological and biochemical activities. A full-factorial central composite design and response surface methodology were used for optimizing three precursors Solanesol, 4-hydroxybenzoic acid and methionine to maximize the production of CoQ10 by Rhodopseudomonas palustris J001. The optimization of the model predicted a maximum response 40.6 [(mg CoQ10)(g dried biomass)-1] CoQ10 production with 124.8 mg l-1 Solanesol, 267.7 mg l-14-hydroxybenzoic acid and 130.2 mg l-1 methionine, respectively. A new combination was prepared according to the result. The observed response was 40.5 ± 0.2 [(mg CoQ10)(g dried biomass)-1] and was 109.8%higher than in the control with no addition of the three precursors.