CAJ | 학술논문

同一种蕈菌子实体,由于外观形貌相似,凭传统外观形貌特征难以鉴别产地来源.用傅里叶变换红外光谱技术结合系统聚类分析了5个不同产地的63个野生小美牛肝菌子实体样本.结果表明,原始红外光谱在4000～400 cm~(-1)总体特征相似,主要是由蛋白质、多糖等吸收谱峰组成,在1800～1000 cm~(-1)范围不同产地样本的原始光谱存在微小的差异.对光谱进行一阶导数和二阶导数处理,用导数光谱进行系统聚类分析(HCA),结果显示,一阶导数光谱在1800～1000 cm~(-1)范围按不同产地样本聚类效果较好,63个样本按5个不同产地很好地聚类,分类正确率达到了90.5%.结果提示傅里叶变换红外光谱结合系统聚类分析可以快速、方便地对不同产地野生蕈菌进行鉴别分类.
동일충심균자실체,유우외관형모상사,빙전통외관형모특정난이감별산지래원.용부리협변환홍외광보기술결합계통취류분석료5개불동산지적63개야생소미우간균자실체양본.결과표명,원시홍외광보재4000～400 cm~(-1)총체특정상사,주요시유단백질、다당등흡수보봉조성,재1800～1000 cm~(-1)범위불동산지양본적원시광보존재미소적차이.대광보진행일계도수화이계도수처리,용도수광보진행계통취류분석(HCA),결과현시,일계도수광보재1800～1000 cm~(-1)범위안불동산지양본취류효과교호,63개양본안5개불동산지흔호지취류,분류정학솔체도료90.5%.결과제시부리협변환홍외광보결합계통취류분석가이쾌속、방편지대불동산지야생심균진행감별분류.
It is hard to differentiate the same species of wild growing mushrooms from different areas by macromorpho-logical features. In this paper, Fourier transform infrared spectroscopy(FTIR) combined with hierarchical cluster analysis was used to identify 63 samples of Boletus speciosus from five different areas. The original characteristic infrared spectras of the mushrooms are very similar, with small differences in the range of 1800 ～ 1000 cm~(-1). Hierarchical cluster analysis was performed using the first derivative and second derivative spectral range 1800 ～ 1000 cm~(-1). The first derivative spectra give satisfactory results. All Boletus speciosus samples were divided into 5 classes with a classification accuracy of 90.5%. It is proved that FTIR spectroscopy combined with hierarchical cluster analysis could be used to discriminate wild growing mushrooms at species level.