CAJ | 학술논문

目的研制一种适用于现场使用的血吸虫抗体快速检测试剂盒.方法通过对原有的斑点免疫金渗滤法全面改进,建立新的快速检测血吸虫抗体试剂盒.采用双盲法,检测慢性血吸虫病人血清100人份,正常人、乙肝患者及其他寄生虫病感染血清140人份.以敏感性、特异性、Youden指数、Kappa值等指标评价试剂盒的检测效果.结果试剂盒的敏感性为92%,特异性为95.08%,Youden指数为0.87,Kappa值为0.87,与华支睾吸虫感染者血清交叉反应率为5%.结论斑点免疫金渗滤法试剂盒血清用量少,反应快,敏感性、特异性较高,适用于现场血吸虫抗体检测.
목적 연제일충괄용우현장사용적혈흡충항체쾌속검측시제합.방법 통과대원유적반점면역금삼려법전면개진,건립신적쾌속검측혈흡충항체시제합.채용쌍맹법,검측만성혈흡충병인혈청100인빈,정상인、을간환자급기타기생충병감염혈청140인빈.이민감성、특이성、Youden지수、Kappa치등지표평개시제합적검측효과.결과 시제합적민감성위92%,특이성위95.08%,Youden지수위0.87,Kappa치위0.87,여화지고흡충감염자혈청교차반응솔위5%.결론 반점면역금삼려법시제합혈청용량소,반응쾌,민감성、특이성교고,괄용우현장혈흡충항체검측.
Objective To develop a rapid kit applied to the field for detection of antibody to schistosome in human sera. Methods A new kit for rapid detection of antibody to schistosome was developed through improving the dot immunogold filtration assay (DIGFA). A total of 100 cases of sera from chronic schistosomiasis patients and 140 from healthy people, HBV patients and the people infected with other parasites were detected by the kit. The sensitivity, specificity, Youden's index and Kappa value were utilized as the evaluation standard. Results The sensitivity of detecting antibody to schistosome, specificity, Youden's index and Kappa value were 92% , 95.08% , 0.87 and 0.87, respectively. The cross reaction to patients with clonorchiasis was 5%. Conclusion DICFA kit is practical for antibody to schistosome detection in the field because of its advantages such as smaller serum needed and faster in reaction.