中国组织化学与细胞化学杂志
中國組織化學與細胞化學雜誌
중국조직화학여세포화학잡지
CHINESE JOURNAL OF HISTOCHEMISY AND CYTOCHEMISY
2010年
1期
11-14
,共4页
徐成胜%吴勇波%何涛%周千星%王德鹏%曾和松
徐成勝%吳勇波%何濤%週韆星%王德鵬%曾和鬆
서성성%오용파%하도%주천성%왕덕붕%증화송
瘦素%胰岛素%IL-6%瘦素受体%基因表达
瘦素%胰島素%IL-6%瘦素受體%基因錶達
수소%이도소%IL-6%수소수체%기인표체
Leptin%Insulin%IL-6%Leptin receptor%Gene expression
目的 研究瘦素、胰岛素及IL-6对平滑肌细胞瘦素受体(Ob-R)mRNA表达的影响.方法 采用六孔板培养皿培养鼠平滑肌细胞,5个孔作为一个浓度组,通过半定量的RT-PCR方法,分别观察了不同浓度的大鼠瘦素、胰岛素及IL-6对平滑肌细胞瘦素受体mRNA表达水平的影响.结果 通过半定量RT-PCR,我们检测了短型瘦素受体(Ob-Ra)和长型瘦素受体(Ob-Rb)的mRNA表达水平,瘦素受体(Ob-R)mRNA表达水平随着瘦素及IL-6浓度增加而上升,随着胰岛素的浓度增加而下降.结论 瘦素及IL-6可在体外上调平滑肌细胞瘦素受体的表达,胰岛素可下调平滑肌细胞瘦素受体的表达.这三个因子参与了瘦素受体的表达调控,对我们了解瘦素抵抗的发生机制有重要意义.
目的 研究瘦素、胰島素及IL-6對平滑肌細胞瘦素受體(Ob-R)mRNA錶達的影響.方法 採用六孔闆培養皿培養鼠平滑肌細胞,5箇孔作為一箇濃度組,通過半定量的RT-PCR方法,分彆觀察瞭不同濃度的大鼠瘦素、胰島素及IL-6對平滑肌細胞瘦素受體mRNA錶達水平的影響.結果 通過半定量RT-PCR,我們檢測瞭短型瘦素受體(Ob-Ra)和長型瘦素受體(Ob-Rb)的mRNA錶達水平,瘦素受體(Ob-R)mRNA錶達水平隨著瘦素及IL-6濃度增加而上升,隨著胰島素的濃度增加而下降.結論 瘦素及IL-6可在體外上調平滑肌細胞瘦素受體的錶達,胰島素可下調平滑肌細胞瘦素受體的錶達.這三箇因子參與瞭瘦素受體的錶達調控,對我們瞭解瘦素牴抗的髮生機製有重要意義.
목적 연구수소、이도소급IL-6대평활기세포수소수체(Ob-R)mRNA표체적영향.방법 채용륙공판배양명배양서평활기세포,5개공작위일개농도조,통과반정량적RT-PCR방법,분별관찰료불동농도적대서수소、이도소급IL-6대평활기세포수소수체mRNA표체수평적영향.결과 통과반정량RT-PCR,아문검측료단형수소수체(Ob-Ra)화장형수소수체(Ob-Rb)적mRNA표체수평,수소수체(Ob-R)mRNA표체수평수착수소급IL-6농도증가이상승,수착이도소적농도증가이하강.결론 수소급IL-6가재체외상조평활기세포수소수체적표체,이도소가하조평활기세포수소수체적표체.저삼개인자삼여료수소수체적표체조공,대아문료해수소저항적발생궤제유중요의의.
Objective To study the effects of leptin, insulin and IL-6 on the mRNA expression of leptin receptor isoforms in rat smooth muscular cells (SMC).Methods Rat smooth muscular cells were cultured in six-well plates and incubated for 24 hours in serum-free media containing leptin, insulin or IL-6 of different concentrations. The mRNA expression of short-form leptin receptors (Ob-Ra) and long-form leptin receptors( Ob-Rb) was detected by semi-quantitatibe RT-PCR.Results Ob-Ra and Ob-Rb expressed in SMC. Ob-R mRNA expression was increased by Leptin and IL-6, but decreased by Insulin.Conclusion In SMC, Ob-R expression was influenced by leptin, IL-6 and insulin. This is vital for us to study the mechanism of leptin resistance .
