CAJ | 학술논문

以不同种源的鹅掌楸、北美鹅掌楸和不同组合的杂交鹅掌楸为试验材料,对176对SSR引物进行初筛、复筛,最后筛选出1对物种特异性SSR引物.该引物在鹅掌楸群体中特异性扩增出190 bp的产物,在北美鹅掌楸群体中特异性扩增出180 bp的产物,在杂交鹅掌楸群体中特异性扩增出180 bp和190 bp 2种产物.该引物对鹅掌楸种间鉴别准确率达100%.
이불동충원적아장추、북미아장추화불동조합적잡교아장추위시험재료,대176대SSR인물진행초사、복사,최후사선출1대물충특이성SSR인물.해인물재아장추군체중특이성확증출190 bp적산물,재북미아장추군체중특이성확증출180 bp적산물,재잡교아장추군체중특이성확증출180 bp화190 bp 2충산물.해인물대아장추충간감별준학솔체100%.
This paper reported that species-specific SSR markers were used to identify Liriodendron tulipifera,L.chinense and hybrid Liriodendron.Overall,90 genotypes were taken as DNA amplification templates,of which 30 came from 20 provenances of L.chinense,30 from 5 provenances of L.tulipifera,and 30 from 30 mating combinations of hybrid Liriodendron.Upon two round amplifying and screening,one species-specific pair primers for Liriodendron were screened from 176 pair primers.With the pair primers,a 190 bp fragment from L.chinense,180 bp fragment from L.tulipifera,and a 190 bp and a 180 bp fragment from hybrid Liriodendron were respectively amplified.To testify the veracity of these pair primers,10 individuals from L.chinense,5 from L.tulipifera,and 10 from hybrid Liriodendron were used as amplification templates,and the results showed 100% veracity for species identification.Therefore,the development of the pair primers provided an effective,stable and reliable method for species-specific identification in Liriodendron at the molecular level.