CAJ | 학술논문

利用MTT法研究了粘细菌Stigmatella WXNXJ-B代谢产物对HepG2肿瘤细胞的抑制作用,考察了一些理化因素对代谢产物稳定性的影响并定性分析了代谢产物.结果表明,Stigmatella WXNXJ-B菌株产生的活性物质能抑制肿瘤细胞的生长,抗肿瘤活性物质产生的最佳发酵时间为7 d.大孔树脂HPD100、XAD16和DA201对活性物质的吸附效果较好,该活性物质在12 h的自然光和紫外光照射下、在温度不高于70 ℃和pH 3～10之间稳定性好,代谢产物中含有内酯类、苷类和甾体类物质.
이용MTT법연구료점세균Stigmatella WXNXJ-B대사산물대HepG2종류세포적억제작용,고찰료일사이화인소대대사산물은정성적영향병정성분석료대사산물.결과표명,Stigmatella WXNXJ-B균주산생적활성물질능억제종류세포적생장,항종류활성물질산생적최가발효시간위7 d.대공수지HPD100、XAD16화DA201대활성물질적흡부효과교호,해활성물질재12 h적자연광화자외광조사하、재온도불고우70 ℃화pH 3～10지간은정성호,대사산물중함유내지류、감류화치체류물질.
In this study, the antitumor effect of the metabolites from Stigmatella WXNXJ-B was analyzing by HepG2 cell using MTT method. Furthermore, the stability of the metabolites under different physical and chemical environment was examined and the qualitative characteristics of the metabolites were analysis. The results showed that the antitumor bioactive metabolites efficient inhibited the growth of HepG2 cell. The optimum fermentation time of antitumor substance was 7 days. HPD100, XAD16 and DA201 resin were efficient to adsorb the bioactive metabolites. The antitumor bioactivity on HepG2 cell line did not obviously change when the temperature was lower 70 ℃, pH was in the range from 3 to 10 and the metabolites were treated with daylight and ultraviolet light during 12 h. It was detected that lactones, glycosides and steroids components existed in the metabolites.