CAJ | 학술논문

目的:观察肺癌患者外周血调节性T细胞的变化,并探讨CpG ODN的干预作用.方法:分离肺癌患者和健康志愿者(各30例)的外周血单个核细胞(PBMC),用流式细胞仪检测CD4~+CD25~+调节性T细胞比例,Real-time PCR检测Foxp3基因的表达,ELISA法检测TGF-β和IFN-γ水平.将30例肺癌患者的PBMC随机分为实验组和安慰剂组,分别给予CpG ODN 2006或安慰剂cpG ODN 1612干预,比较十预前后上述指标的变化.结果:肺癌患者PBMC中的CD4~+CD25~+调节性T细胞比例、Foxp3基因的相对表达量、TGF-β水平均高于健康对照组,差异具有显著性,但不同病理分型和分期的肺癌患者亚组间比较无显著性差异.两组的IFN-γ水平比较无差异性.CpG ODN干预后CpG ODN 2006治疗组的CD4~+CD25~+调节性T细胞比例、Foxp3基因的相对表达量及TGF-β水平出现了下降,差异有显著性,IFN-γ水平变化无显著性差异.而CpG ODN 1612安慰剂组的上述指标在干预前后则无显著性改变.结论:肺癌患者外周血中的CD4~+CD25~+Foxp3~+调节性T细胞比例、TGF-β水平明显升高.cpG ODN 2006干预可下调CD4~+CD25~+Foxp3~+调节性T细胞比例及TGF-β水平.
목적:관찰폐암환자외주혈조절성T세포적변화,병탐토CpG ODN적간예작용.방법:분리폐암환자화건강지원자(각30례)적외주혈단개핵세포(PBMC),용류식세포의검측CD4~+CD25~+조절성T세포비례,Real-time PCR검측Foxp3기인적표체,ELISA법검측TGF-β화IFN-γ수평.장30례폐암환자적PBMC수궤분위실험조화안위제조,분별급여CpG ODN 2006혹안위제cpG ODN 1612간예,비교십예전후상술지표적변화.결과:폐암환자PBMC중적CD4~+CD25~+조절성T세포비례、Foxp3기인적상대표체량、TGF-β수평균고우건강대조조,차이구유현저성,단불동병리분형화분기적폐암환자아조간비교무현저성차이.량조적IFN-γ수평비교무차이성.CpG ODN간예후CpG ODN 2006치료조적CD4~+CD25~+조절성T세포비례、Foxp3기인적상대표체량급TGF-β수평출현료하강,차이유현저성,IFN-γ수평변화무현저성차이.이CpG ODN 1612안위제조적상술지표재간예전후칙무현저성개변.결론:폐암환자외주혈중적CD4~+CD25~+Foxp3~+조절성T세포비례、TGF-β수평명현승고.cpG ODN 2006간예가하조CD4~+CD25~+Foxp3~+조절성T세포비례급TGF-β수평.
Objective:To study the change of regulatory T cells in PBMC and the effects of CpG ODN in patients with lung cancer.Methods: Lymphocytes isolated from blood of 30 patients with lung canaer and 30 healthy volunteers were analyzed for the proportion of CD4 ~+CD25~+ T cells by flow cytometry.The mRNA expression of Foxp3 was detected by real-time PCR and the levels of TGF-β and IFN-γ were tested by ELLSA.The PBMC of 30 patients with lung cancer were randomly divided into treated group(CpG ODN 2006)and placebo group(CpG ODN 1612).The proportion of CD4 ~+ CD25~+ T cells,the mRNA expression of Foxp3 and the levels of TGF-β and IFN-γ were compared before and after treatment.Results:Tbe proportion of CD4~+ CD25~+ T cells,the mRNA expression of Foxp3 and the levels of TGF-β in patients with lung cancer were higher than those in healthy volunteers,but there was no significant difference in such parameters among subgroups of pathologic classification and clinical stage patients.The proportion of CD4 ~+ CD25~+ T cells, the mRNA expression of Foxp3 and the levels of TGF-β were dropped in CpG ODN 2006 group after treatment.There was no significant difference in these parameters before and after treatment in CpG ODN 1612 group.Conclusion: The proportion of CD4~+ CD25 ~+ T cells, the mRNA expression of Foxp3 and the levels of TGF-β of PBMC in lung cancer patients are higher than those in healthy volunteers.Treating by CpG ODN 2006 could down-regulate the proportion of CD4~+ CD25~+ Foxp3~+ regulatory T cells and the levels of TGF-β of PBMC from the patients with lung cancer.