CAJ | 학술논문

[目的]对拟南芥Rac/Rop GEF1(简称GEF1)基因在Rac/Rop GTPse介导的生长素信号途径等方面所起的作用进行初步探索.[方法]利用实验室已构建的稳定表达的拟南芥GEF1基因启动子与GUS融合的转基因植株和GEF1基因过表达的转基因植株,通过GUS组织化学染色分析GEF1基因的表达模式,并对GEF1基因过表达植株的幼苗根系发育情况进行分析.[结果]GEF1基因主要在幼苗根的分生组织和维管组织细胞、侧根原基和根毛细胞表达;经生长素(NAA)诱导处理后,在上述细胞中的表达显著增强.过表达GEF1基因促进侧根的形成.[结论]GEF1基因的功能与根和根毛细胞的发育有关,GEF1基因可能参与对侧根发育的调控.
[목적]대의남개Rac/Rop GEF1(간칭GEF1)기인재Rac/Rop GTPse개도적생장소신호도경등방면소기적작용진행초보탐색.[방법]이용실험실이구건적은정표체적의남개GEF1기인계동자여GUS융합적전기인식주화GEF1기인과표체적전기인식주,통과GUS조직화학염색분석GEF1기인적표체모식,병대GEF1기인과표체식주적유묘근계발육정황진행분석.[결과]GEF1기인주요재유묘근적분생조직화유관조직세포、측근원기화근모세포표체;경생장소(NAA)유도처리후,재상술세포중적표체현저증강.과표체GEF1기인촉진측근적형성.[결론]GEF1기인적공능여근화근모세포적발육유관,GEF1기인가능삼여대측근발육적조공.
[Objective] The aim was to research the function of AtGEF1 in Rac/Rop GTPses mediate auxin signal passway. [Method] Using the transgenic plants of AtGEF1 promotor fused with GUS reporter gene and the over-expression plants of Rac/Rop GEF1 under the control of 35S promoter as materials, which were constructed from our lab, the expression pattern of GEF1 was analyzed by GUS assay using histochemical staining, and the development of seedling roots of over-expression plant of GEF1 was observed. [Result] GEF1 expression was mainly detected in root meristem, root vascular tissue, lateral roots and root hair. Furthermore, the expression level of GEF1 was highly increased with the induction of NAA. Over-expression of GEF1 was observed to enhance lateral root formation. [Conclusion] GEF1 may be involved in the regulation of development of root and root hair, and it may have redundant function in the control of lateral root development.