中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2008年
6期
409-412
,共4页
重组蛋白质类%蝎毒抗癌多肽%肺肿瘤%A549细胞%抗肿瘤%细胞增殖%细胞凋亡%钙离子
重組蛋白質類%蝎毒抗癌多肽%肺腫瘤%A549細胞%抗腫瘤%細胞增殖%細胞凋亡%鈣離子
중조단백질류%갈독항암다태%폐종류%A549세포%항종류%세포증식%세포조망%개리자
Recombinant proteins%Antineoplastic polypeptide of buthus martensii venom%Lungneoplasms%A549 cells%Antitumor%Cell proliferation%Apoptosis%Calcium
目的 探讨蝎毒重组蛋白对人肺腺癌A549细胞的抑制作用及其抗肿瘤机制.方法 四甲基偶氮唑蓝(MTT)比色法检测蝎毒重组蛋白对人肺癌A549细胞增殖的抑制作用;荧光显微镜及流式细胞仪(FCM)技术观测蝎毒重组蛋白对肿瘤细胞内钙离子浓度([Ca2+]I)的影响,探讨[Ca2+]I变化时Ca2+的可能来源.结果 蝎毒重组蛋白对人肺癌A549细胞生长有明显抑制作用;荧光显微镜显示,蝎毒重组蛋白作用细胞48 h后细胞内钙离子荧光强度增强,提示[Ca2+]I升高;流式细胞仪显示蝎毒重组蛋白在不同情况下可显著增加肿瘤[Ca2+]I(P<0.05).结论 蝎毒重组蛋白对人肺腺癌A549细胞增殖具有显著抑制作用,可能与升高肿瘤[Ca2+]I继而诱导肿瘤细胞凋亡有关;其升高肿瘤[Ca2+]I是通过开放肿瘤细胞膜钙通道和肿瘤细胞内钙库释放两条途径实现.
目的 探討蝎毒重組蛋白對人肺腺癌A549細胞的抑製作用及其抗腫瘤機製.方法 四甲基偶氮唑藍(MTT)比色法檢測蝎毒重組蛋白對人肺癌A549細胞增殖的抑製作用;熒光顯微鏡及流式細胞儀(FCM)技術觀測蝎毒重組蛋白對腫瘤細胞內鈣離子濃度([Ca2+]I)的影響,探討[Ca2+]I變化時Ca2+的可能來源.結果 蝎毒重組蛋白對人肺癌A549細胞生長有明顯抑製作用;熒光顯微鏡顯示,蝎毒重組蛋白作用細胞48 h後細胞內鈣離子熒光彊度增彊,提示[Ca2+]I升高;流式細胞儀顯示蝎毒重組蛋白在不同情況下可顯著增加腫瘤[Ca2+]I(P<0.05).結論 蝎毒重組蛋白對人肺腺癌A549細胞增殖具有顯著抑製作用,可能與升高腫瘤[Ca2+]I繼而誘導腫瘤細胞凋亡有關;其升高腫瘤[Ca2+]I是通過開放腫瘤細胞膜鈣通道和腫瘤細胞內鈣庫釋放兩條途徑實現.
목적 탐토갈독중조단백대인폐선암A549세포적억제작용급기항종류궤제.방법 사갑기우담서람(MTT)비색법검측갈독중조단백대인폐암A549세포증식적억제작용;형광현미경급류식세포의(FCM)기술관측갈독중조단백대종류세포내개리자농도([Ca2+]I)적영향,탐토[Ca2+]I변화시Ca2+적가능래원.결과 갈독중조단백대인폐암A549세포생장유명현억제작용;형광현미경현시,갈독중조단백작용세포48 h후세포내개리자형광강도증강,제시[Ca2+]I승고;류식세포의현시갈독중조단백재불동정황하가현저증가종류[Ca2+]I(P<0.05).결론 갈독중조단백대인폐선암A549세포증식구유현저억제작용,가능여승고종류[Ca2+]I계이유도종류세포조망유관;기승고종류[Ca2+]I시통과개방종류세포막개통도화종류세포내개고석방량조도경실현.
Objective To investigate the inhibitory effect and antitumor mechanism of recombinant protein of buthus martensii venom on A549 human lung adenocarcinoma cell line.Methods The inhibitionof proliferation in vitro was measured by methyl thiazolyl tetrazolium (MTT)assay.Influence of [ Ca2+]i by recombinant protein of buthus martensii venom was observed by fluorescence microscope and flow cytometry.And then to explore the source of Ca2+ when[Ca2+]i was aherating.Results The protein could inhibit A549cell proliferation.Fluorescent microscope assay showed enhanced fluorescent intensity after 48 hours,and itmeaned the increasing[Ca2+]i concentration.Flow cytometry assays showed the recombinant protein can significantly increase the concentration of[ca2+]i in tumor cells under different circumstances(P<0.05).Conclusion The recombinant protein can obviously inhibit the proliferation of A549 cell line,and itsmechanism has relationship with tumor cell apoptosis through increasing[Ca+]i level of tumor cell asresulted by openning the calcium ion channel on the membrane and releasing calcium ion in tumor cells.
