中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2011年
10期
583-588
,共6页
朱雯%居丽雯%蒋露芳%熊海燕%汪千力%姜庆五
硃雯%居麗雯%蔣露芳%熊海燕%汪韆力%薑慶五
주문%거려문%장로방%웅해연%왕천력%강경오
手足口病%肠道病毒71型,人%基因型%序列同源性,核酸%序列分析
手足口病%腸道病毒71型,人%基因型%序列同源性,覈痠%序列分析
수족구병%장도병독71형,인%기인형%서렬동원성,핵산%서렬분석
Hand,foot and mouth disease%Enterovirus 71,human%Genotype%Sequencehomology,nacleic acid%Sequence analysis
目的 了解2010年上海地区手足口病患者中肠道病毒71型(EV71)分离株VP1区基因特征.方法 选取2010年不同时间的轻症与重症病例EV71分离株18株,对其抗原决定簇部位VP1区进行核苷酸序列测定、比对,并与美国国立生物技术信息中心公布的EV71 A、B、C各基因型分离株序列进行比对,构建系统发生树.结果 上海地区18株病毒分离株与A、B基因型参考毒株的核苷酸同源性分别为81.5％～82.6％、83.4％～84.2％,氨基酸同源性分别为94.3％～95.0％、96.6％～97.0％,与C基因型比较,核苷酸与氨基酸同源性分别为87.4％～99.2％与98.7％～100.0％.而与2008年安徽省阜阳市的EV71流行株(C4亚型)的核苷酸同源性可达97.8％～99.2％,氨基酸同源性99.3％～100.0％.在系统发生树上,这18株毒株均在C基因型中,与C4亚型属同一分支.重症病例与轻症病例EV71分离株在VP1基因区并无明显差异.结论 上海地区手足口病患者中分离的EV71流行株应属C基因型的C4亚型.
目的 瞭解2010年上海地區手足口病患者中腸道病毒71型(EV71)分離株VP1區基因特徵.方法 選取2010年不同時間的輕癥與重癥病例EV71分離株18株,對其抗原決定簇部位VP1區進行覈苷痠序列測定、比對,併與美國國立生物技術信息中心公佈的EV71 A、B、C各基因型分離株序列進行比對,構建繫統髮生樹.結果 上海地區18株病毒分離株與A、B基因型參攷毒株的覈苷痠同源性分彆為81.5％～82.6％、83.4％～84.2％,氨基痠同源性分彆為94.3％～95.0％、96.6％～97.0％,與C基因型比較,覈苷痠與氨基痠同源性分彆為87.4％～99.2％與98.7％～100.0％.而與2008年安徽省阜暘市的EV71流行株(C4亞型)的覈苷痠同源性可達97.8％～99.2％,氨基痠同源性99.3％～100.0％.在繫統髮生樹上,這18株毒株均在C基因型中,與C4亞型屬同一分支.重癥病例與輕癥病例EV71分離株在VP1基因區併無明顯差異.結論 上海地區手足口病患者中分離的EV71流行株應屬C基因型的C4亞型.
목적 료해2010년상해지구수족구병환자중장도병독71형(EV71)분리주VP1구기인특정.방법 선취2010년불동시간적경증여중증병례EV71분리주18주,대기항원결정족부위VP1구진행핵감산서렬측정、비대,병여미국국립생물기술신식중심공포적EV71 A、B、C각기인형분리주서렬진행비대,구건계통발생수.결과 상해지구18주병독분리주여A、B기인형삼고독주적핵감산동원성분별위81.5％～82.6％、83.4％～84.2％,안기산동원성분별위94.3％～95.0％、96.6％～97.0％,여C기인형비교,핵감산여안기산동원성분별위87.4％～99.2％여98.7％～100.0％.이여2008년안휘성부양시적EV71류행주(C4아형)적핵감산동원성가체97.8％～99.2％,안기산동원성99.3％～100.0％.재계통발생수상,저18주독주균재C기인형중,여C4아형속동일분지.중증병례여경증병례EV71분리주재VP1기인구병무명현차이.결론 상해지구수족구병환자중분리적EV71류행주응속C기인형적C4아형.
Objective To study the gene characteristics of VP1 region of Enterovirus 71 (EV71) strains isolated from clinical specimens of children with hand-foot-and-mouth disease(HFMD) in Shanghai in 2010.Methods Eighteen EV71 isolates were selected from different periods of year 2010,including strains isolated from fatal cases and non-fatal cases.Complete VP1 gene (891nucleotides) of the eighteen EV71 isolates were amplified and sequenced,and then compared with that of genotype A,B,C reference EV71 strains in GeneBank by homogeneity and phylogenetic tree analyses.Results The nucleotide homogeneities between these 18 Shanghai strains and the representative isolates of genotype A and B were 81.5 ％ -82.6 ％ and 83.4 ％- 84.2 ％,respectively,while the amino acid homogeneities were 94.3 ％- 95.0％ and 96.6％ -97.0％,respectively.The nucleotide and amino acid homogeneities between the 18 Shanghai strains and the representative isolates of genotype C were 87.4％- 99.2％ and 98.7％ -100.0％,respectively.Of note,the nucleotide and amino acid homogeneities between Shanghai strains and Fuyang EV71 strains (representative strain of C4 subtype) appeared to be 97.8％- 99.2％ and 99.3％- 100.0％,respectively.The eighteen EV71 Shanghai strains were classified as genotype C,subgenogroup C4 in the phylogenetic tree.There was no remarkable difference in VP1 gene between the strains isolated from fatal cases and non-fatal cases.Conclusion The EV71 strains isolated from Shanghai belong to subgenogroup C4.
