中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2012年
8期
840-845
,共6页
急性肺损伤%急性呼吸窘迫综合征%肺纤维化%血必净注射液%油酸%Ⅲ前胶原蛋白%HE染色%Masson染色
急性肺損傷%急性呼吸窘迫綜閤徵%肺纖維化%血必淨註射液%油痠%Ⅲ前膠原蛋白%HE染色%Masson染色
급성폐손상%급성호흡군박종합정%폐섬유화%혈필정주사액%유산%Ⅲ전효원단백%HE염색%Masson염색
Acute lung injury%Acute respiratory distress syndrome%Pulmonary fibrosis%Xuebijing%Oleic acid%Ⅲ procollagen%HE staining%Masson staining
目的 探讨血必净对油酸诱导大鼠急性肺损伤后肺纤维化的影响.方法 雄性SPF级Wistar大鼠60只,随机(随机数字法)分为3组(n=20):A组注射生理盐水作为健康对照组；B、C两组所有大鼠均用油酸复制急性肺损伤模型,B组为模型对照组,C组在模型基础上加用血必净为干预组.3组均于注射后12 h抽动脉血作血气分析测定血氧分压,以氧合指数≤300为造模成功.确定模型成功后,A、B两组经尾静脉注射生理盐水(4 ml/kg),C组经尾静脉给予血必净(4 ml/kg),每天1次,连续给药7d.各组于给药后24h、7d、14 d和28 d各处死5只大鼠,取心脏血后处死大鼠,左肺称湿质量后行支气管肺泡灌洗,灌洗后烘干称质量,酶联免疫法(ELISA)检测血清和支气管肺泡灌洗液Ⅲ前胶原(PCⅢ)浓度,取右肺上叶行肺组织病理学检查,经石蜡包埋切片后分别行苏木素-伊红(HE)染色和Masson染色.各组间比较采用单因素方差分析,均数间差别多重比较采用SNK-q检验；非正态分布或方差不齐采用秩和检验.所有数据均用SPSS 13.0统计软件包进行统计处理.结果 B、C两组氧合指数(PaO2/FiO2)＜300,与A组比较明显下降(P＜0.05)；与A组相比,B、C组在7、14、28 d3个时点血清和支气管肺泡灌洗液中Ⅲ前胶原蛋白的浓度增高(P＜0.01),C组与B组相比在14 d和28 d两时点血清PCⅢ浓度降低(P＜0.05),C组在7、1d和28 d时点支气管肺泡灌洗中Ⅲ前胶原蛋白的浓度比同时段的B组降低(P＜0.01).结论 血必净可能通过抑制急性肺损伤后肺纤维化而有助于损伤肺保护.
目的 探討血必淨對油痠誘導大鼠急性肺損傷後肺纖維化的影響.方法 雄性SPF級Wistar大鼠60隻,隨機(隨機數字法)分為3組(n=20):A組註射生理鹽水作為健康對照組；B、C兩組所有大鼠均用油痠複製急性肺損傷模型,B組為模型對照組,C組在模型基礎上加用血必淨為榦預組.3組均于註射後12 h抽動脈血作血氣分析測定血氧分壓,以氧閤指數≤300為造模成功.確定模型成功後,A、B兩組經尾靜脈註射生理鹽水(4 ml/kg),C組經尾靜脈給予血必淨(4 ml/kg),每天1次,連續給藥7d.各組于給藥後24h、7d、14 d和28 d各處死5隻大鼠,取心髒血後處死大鼠,左肺稱濕質量後行支氣管肺泡灌洗,灌洗後烘榦稱質量,酶聯免疫法(ELISA)檢測血清和支氣管肺泡灌洗液Ⅲ前膠原(PCⅢ)濃度,取右肺上葉行肺組織病理學檢查,經石蠟包埋切片後分彆行囌木素-伊紅(HE)染色和Masson染色.各組間比較採用單因素方差分析,均數間差彆多重比較採用SNK-q檢驗；非正態分佈或方差不齊採用秩和檢驗.所有數據均用SPSS 13.0統計軟件包進行統計處理.結果 B、C兩組氧閤指數(PaO2/FiO2)＜300,與A組比較明顯下降(P＜0.05)；與A組相比,B、C組在7、14、28 d3箇時點血清和支氣管肺泡灌洗液中Ⅲ前膠原蛋白的濃度增高(P＜0.01),C組與B組相比在14 d和28 d兩時點血清PCⅢ濃度降低(P＜0.05),C組在7、1d和28 d時點支氣管肺泡灌洗中Ⅲ前膠原蛋白的濃度比同時段的B組降低(P＜0.01).結論 血必淨可能通過抑製急性肺損傷後肺纖維化而有助于損傷肺保護.
목적 탐토혈필정대유산유도대서급성폐손상후폐섬유화적영향.방법 웅성SPF급Wistar대서60지,수궤(수궤수자법)분위3조(n=20):A조주사생리염수작위건강대조조；B、C량조소유대서균용유산복제급성폐손상모형,B조위모형대조조,C조재모형기출상가용혈필정위간예조.3조균우주사후12 h추동맥혈작혈기분석측정혈양분압,이양합지수≤300위조모성공.학정모형성공후,A、B량조경미정맥주사생리염수(4 ml/kg),C조경미정맥급여혈필정(4 ml/kg),매천1차,련속급약7d.각조우급약후24h、7d、14 d화28 d각처사5지대서,취심장혈후처사대서,좌폐칭습질량후행지기관폐포관세,관세후홍간칭질량,매련면역법(ELISA)검측혈청화지기관폐포관세액Ⅲ전효원(PCⅢ)농도,취우폐상협행폐조직병이학검사,경석사포매절편후분별행소목소-이홍(HE)염색화Masson염색.각조간비교채용단인소방차분석,균수간차별다중비교채용SNK-q검험；비정태분포혹방차불제채용질화검험.소유수거균용SPSS 13.0통계연건포진행통계처리.결과 B、C량조양합지수(PaO2/FiO2)＜300,여A조비교명현하강(P＜0.05)；여A조상비,B、C조재7、14、28 d3개시점혈청화지기관폐포관세액중Ⅲ전효원단백적농도증고(P＜0.01),C조여B조상비재14 d화28 d량시점혈청PCⅢ농도강저(P＜0.05),C조재7、1d화28 d시점지기관폐포관세중Ⅲ전효원단백적농도비동시단적B조강저(P＜0.01).결론 혈필정가능통과억제급성폐손상후폐섬유화이유조우손상폐보호.
Objective To investigate the effect of Xuebijing (Chinese herbal medicine preparation) on lung fibrosis in a rat model of oleic acid - induced lung injury.Methods Sixty SPF level of male Wistar rats were randomly ( random number) divided into three groups ( n =20 ):normal control group ( group A),oleic acid model group (group B),oleic acid and Xuebijing group (group C).Rats in group A were injected with normal saline (0.13 ml/kg) ; acute lung injury (ALI) model rats were injected with oleic acid (0. 13 ml/kg) intravenously in B and C groups.Twelve hours after administrations of saline or oleic acid into rats of three groups separately,samples of arterial blood from all rats were taken for blood gas analysis.By analysis of blood oxygen partial pressure,the acute lung injury model successfully made was confirmed.Then the rats in group C were treated with xuebijing (4 ml/kg) intravenously,once a day from the 1st to 7th day.The rats in groups A and B were treated with saline (4 ml/kg).Five rats in each group were randomly sacrificed on the 1st,7th,14th,28th day,separately.The bronchoalveolar lavage (BAL) of left lung of all rats was carried out after weight of lung weighed.The lefi lungs of rats were weighed in wet and dry in order to get the wet/dry ratio.Venous blood and BAL fluid (BALF) were obtained for detection of procollagen Ⅲ ( PC Ⅲ ) concentrations with enzyme - linked immunosorbent assay (ELISA).Right upper lobe of lung specimens was taken for histopathological examination after paraffin - embedded sections with hematoxylin-eosin (HE) staining and Masson staining.Multiple comparisons were analyzed by using oneway analysis of variance (SNK-q test).Statistical analyses were performed by using SPSS 13.0 software.Results Oxygenation index of groups B and C ( PaO2/FiO2 ＜ 300) significantly decreased compared with the group A ( P ＜ 0.01 ).Levels of serum PC Ⅲ on the 14th day and 28th day in group C decreased significantly in comparison with group B ( P ＜ 0.05 ).Compared with group A,the levels of BALF PC Ⅲ and serum PC Ⅲ in group B and group C on the 7th day,14th day and 28th day increased ( P ＜ 0.01 ).Levels of PC Ⅲ in BALF on the 7th day,14th day and 28th day in C group decreased in comparison with group B ( P ＜0.01 ).Compared with group A,the levels of PCⅢ in BALF in group B and group C on the 7th day,14th day and 28th day increased ( P ＜ 0.05 ).Conclusions The result suggests that treatment with xuebijing may be useful in inhibiting pulmonary fibrosis during the course of ALI in rots.
