中华糖尿病杂志
中華糖尿病雜誌
중화당뇨병잡지
CHINES JOURNAL OF DLABETES MELLITUS
2010年
3期
183-186
,共4页
汤绍芳%卫红艳%张鹏%高志红%邱明才
湯紹芳%衛紅豔%張鵬%高誌紅%邱明纔
탕소방%위홍염%장붕%고지홍%구명재
白细胞介素-6%多态性,单核苷酸%2型糖尿病%下肢血管病变
白細胞介素-6%多態性,單覈苷痠%2型糖尿病%下肢血管病變
백세포개소-6%다태성,단핵감산%2형당뇨병%하지혈관병변
Interleukin-6%Polymorphism single nucleotide%Type 2 diabetes mellitus%Vascular disease of low extremities
目的 从病因学角度探讨白细胞介素-6(IL-6)基因启动子-572C/G多态性与2型糖尿病并发下肢血管病变间的关系.方法 选取2008年2月至2009年1月天津医科大学总医院内分泌科住院的2型糖尿病患者280例,其中男130例,年龄(58.7±0.8)岁;女150例,年龄(58.1±0.5)岁.根据血管彩色多普勒超声检查分为下肢血管病变(VDLE)组、下肢血管神经病变(VNDLE)组、下肢神经病变(NDLE)组和无下肢并发症(NC)组.另选取2008年12月至2009年1月社区健康查体正常的人群70例作为正常对照组.取受试者静脉血提取基因组DNA,聚会酶链反应-限制性片段长度多态性(PCR-RFLP)法测定IL-6启动子区-572C/G基因多态性.各组间频率比较用X2检验,不同组间的计量资料均数比较采用单因素方差分析.结果 IL-6启动子-572C/G具有多态性分布的特点.基因型GG和CG,等位基因G频率在糖尿病组(32.1%CC,49.3%CG,18.6%GG;43.2%G,56.8%C)高于正常对照组(54.3%CC,37.1%CG,8.6%GG;27.1%G,72.9%C),差异有统计学意义(X2值分别为12.649和12.054,P<0.05);在糖尿病各组和正常对照组间差异有统计学意义(X2值分别为22.015和20.471,P<0.05);在下肢血管病变组(23.4%CC,53.9%CG,22.7%GG;49.6%G,50.4%C)高于无下肢血管病变组(36.7%CC,47.5%CG,15.8%GG;60.4%G,39.6%C),差异有统计学意义(X2值分别为6.399和5.752,P<0.05).结论 IL-6启动子-572C/G的基因型GG和CG,等位基因G可能是2型糖尿病合并下肢血管病变的易感基因,IL-6启动子-572C/G基因多态性与2型糖尿病下肢血管病变密切相关.
目的 從病因學角度探討白細胞介素-6(IL-6)基因啟動子-572C/G多態性與2型糖尿病併髮下肢血管病變間的關繫.方法 選取2008年2月至2009年1月天津醫科大學總醫院內分泌科住院的2型糖尿病患者280例,其中男130例,年齡(58.7±0.8)歲;女150例,年齡(58.1±0.5)歲.根據血管綵色多普勒超聲檢查分為下肢血管病變(VDLE)組、下肢血管神經病變(VNDLE)組、下肢神經病變(NDLE)組和無下肢併髮癥(NC)組.另選取2008年12月至2009年1月社區健康查體正常的人群70例作為正常對照組.取受試者靜脈血提取基因組DNA,聚會酶鏈反應-限製性片段長度多態性(PCR-RFLP)法測定IL-6啟動子區-572C/G基因多態性.各組間頻率比較用X2檢驗,不同組間的計量資料均數比較採用單因素方差分析.結果 IL-6啟動子-572C/G具有多態性分佈的特點.基因型GG和CG,等位基因G頻率在糖尿病組(32.1%CC,49.3%CG,18.6%GG;43.2%G,56.8%C)高于正常對照組(54.3%CC,37.1%CG,8.6%GG;27.1%G,72.9%C),差異有統計學意義(X2值分彆為12.649和12.054,P<0.05);在糖尿病各組和正常對照組間差異有統計學意義(X2值分彆為22.015和20.471,P<0.05);在下肢血管病變組(23.4%CC,53.9%CG,22.7%GG;49.6%G,50.4%C)高于無下肢血管病變組(36.7%CC,47.5%CG,15.8%GG;60.4%G,39.6%C),差異有統計學意義(X2值分彆為6.399和5.752,P<0.05).結論 IL-6啟動子-572C/G的基因型GG和CG,等位基因G可能是2型糖尿病閤併下肢血管病變的易感基因,IL-6啟動子-572C/G基因多態性與2型糖尿病下肢血管病變密切相關.
목적 종병인학각도탐토백세포개소-6(IL-6)기인계동자-572C/G다태성여2형당뇨병병발하지혈관병변간적관계.방법 선취2008년2월지2009년1월천진의과대학총의원내분비과주원적2형당뇨병환자280례,기중남130례,년령(58.7±0.8)세;녀150례,년령(58.1±0.5)세.근거혈관채색다보륵초성검사분위하지혈관병변(VDLE)조、하지혈관신경병변(VNDLE)조、하지신경병변(NDLE)조화무하지병발증(NC)조.령선취2008년12월지2009년1월사구건강사체정상적인군70례작위정상대조조.취수시자정맥혈제취기인조DNA,취회매련반응-한제성편단장도다태성(PCR-RFLP)법측정IL-6계동자구-572C/G기인다태성.각조간빈솔비교용X2검험,불동조간적계량자료균수비교채용단인소방차분석.결과 IL-6계동자-572C/G구유다태성분포적특점.기인형GG화CG,등위기인G빈솔재당뇨병조(32.1%CC,49.3%CG,18.6%GG;43.2%G,56.8%C)고우정상대조조(54.3%CC,37.1%CG,8.6%GG;27.1%G,72.9%C),차이유통계학의의(X2치분별위12.649화12.054,P<0.05);재당뇨병각조화정상대조조간차이유통계학의의(X2치분별위22.015화20.471,P<0.05);재하지혈관병변조(23.4%CC,53.9%CG,22.7%GG;49.6%G,50.4%C)고우무하지혈관병변조(36.7%CC,47.5%CG,15.8%GG;60.4%G,39.6%C),차이유통계학의의(X2치분별위6.399화5.752,P<0.05).결론 IL-6계동자-572C/G적기인형GG화CG,등위기인G가능시2형당뇨병합병하지혈관병변적역감기인,IL-6계동자-572C/G기인다태성여2형당뇨병하지혈관병변밀절상관.
Objective To investigate the polymorphism of the interleukin-6 promoter-572C/G and its relationship with type 2 diabetes mellitus(T2DM)complicated with vascular disease of lower extremities.Methods Two hundreds and eighty hospitalized T2DM patients from the Department of Endocrinology in General Hospital of Tianjin Medical University were recruited from February 2008 to January 2009.One hundred and thirty cases were male in(58.7±0.8)years and 150 cases were female(mesh age(58.1 ±0.5)years).Based upon the ultrasound test of the lower extremities.the participants were divided into vascular disease of lower extremities(VDLE)group,vascular and neuropathic disease of lower extremities (VNDLE)group,neuropathic disease of lower extremities(NDLE)group and non-complications group (NC).Seventy people for health care were non-randomly recruited as healthy control group.By PCR-RFLP technique.the polymorphism of the human IL-6 gene-572C/G in 70 health controls and 280 type 2 diabetic patients was evaluated.The genotype and allele frequency in different groups was evaluated by X2 teal The measurement data in different groups was evaluated by analysis of variance.Results The IL-6 promoter572C/G had polymorphism.The frequeney of genotype GG and CG,as well as allele G,had statistics difference between T2DM group and healthy control group(X2=12.649,12.054,respectively,all P<0.05).And so did they in T2DM patients who complicated with different vascular disease of the lower extremities compared with healthy control group(X2=22.015,20.471,respectively,all P<0.05).The genotype and allele frequency was also higher in T2DM patients complicated with vascular disease of lower extremities than those without the complications(X2=6.399,5.752,respectively,all P<0.05).Conclusion The genotype GG and CG and the allele G of the Interleukin-6 promoter-572C/G might be the susceptibility gene of the T2DM complicated with vascular disease of the lower extremities.The polymorphism of the Interleukin-6 promoter-572c/G might has great correlation with T2DM complicated with vascular disease of the low extremities.
