中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2009年
6期
495-498
,共4页
孙肖红%付士红%王静林%吕新军%王焕琴%何英%翟友刚%梁国栋
孫肖紅%付士紅%王靜林%呂新軍%王煥琴%何英%翟友剛%樑國棟
손초홍%부사홍%왕정림%려신군%왕환금%하영%적우강%량국동
版纳病毒%系统进化分析
版納病毒%繫統進化分析
판납병독%계통진화분석
Banna virus%Phylogenetie analysis
目的 对滇西北地区分离的版纳病毒进行系统鉴定,明确与云南其他地区分离株的差异.方法 对2005、2006年从滇西北地区分离的3株版纳病毒进行形态学、基因组带型和分子生物学鉴定,测定第12片段核苷酸序列,进行序列的同源性和系统进化分析.结果 从2005、2006年滇西北蚊虫标本中分离到的3株版纳病毒为直径约70 nm、无包膜、有双层衣壳的球形颗粒,基因组带型为6-6分布.3株版纳病毒问第12片段核苷酸序列同源性为99%,与云南其他地区和印度尼西亚版纳病毒毒株的最大同源性分别为98%和90%;系统进化分析显示滇西北分离株和云南其他地区分离株共同形成一个分枝;第12片段编码的氨基酸序列比较发现我国版纳分离株和印度尼西亚毒株间存在共同的氨基酸残基差异.结论 首次从滇西北地区蚊虫中分离到3株版纳病毒,基因序列分析显示滇两北分离株和云南其他地区分离株同源性较高.
目的 對滇西北地區分離的版納病毒進行繫統鑒定,明確與雲南其他地區分離株的差異.方法 對2005、2006年從滇西北地區分離的3株版納病毒進行形態學、基因組帶型和分子生物學鑒定,測定第12片段覈苷痠序列,進行序列的同源性和繫統進化分析.結果 從2005、2006年滇西北蚊蟲標本中分離到的3株版納病毒為直徑約70 nm、無包膜、有雙層衣殼的毬形顆粒,基因組帶型為6-6分佈.3株版納病毒問第12片段覈苷痠序列同源性為99%,與雲南其他地區和印度尼西亞版納病毒毒株的最大同源性分彆為98%和90%;繫統進化分析顯示滇西北分離株和雲南其他地區分離株共同形成一箇分枝;第12片段編碼的氨基痠序列比較髮現我國版納分離株和印度尼西亞毒株間存在共同的氨基痠殘基差異.結論 首次從滇西北地區蚊蟲中分離到3株版納病毒,基因序列分析顯示滇兩北分離株和雲南其他地區分離株同源性較高.
목적 대전서북지구분리적판납병독진행계통감정,명학여운남기타지구분리주적차이.방법 대2005、2006년종전서북지구분리적3주판납병독진행형태학、기인조대형화분자생물학감정,측정제12편단핵감산서렬,진행서렬적동원성화계통진화분석.결과 종2005、2006년전서북문충표본중분리도적3주판납병독위직경약70 nm、무포막、유쌍층의각적구형과립,기인조대형위6-6분포.3주판납병독문제12편단핵감산서렬동원성위99%,여운남기타지구화인도니서아판납병독독주적최대동원성분별위98%화90%;계통진화분석현시전서북분리주화운남기타지구분리주공동형성일개분지;제12편단편마적안기산서렬비교발현아국판납분리주화인도니서아독주간존재공동적안기산잔기차이.결론 수차종전서북지구문충중분리도3주판납병독,기인서렬분석현시전량북분리주화운남기타지구분리주동원성교고.
Objective To identify the Banna viruses isolated in northwestern part of Yunnan prov-ince in order to make the difference clear between the isolates and other Banna viruses isolated in other parts of Yunnan. Methods Three isolates of Banna vires isolated in 2005 and 2006 were identified by morpholo-gy, RNA-PAGE profile and molecular biologic method. Nueleotide and amino acid sequences of segment 12 of the 3 isolates were sequenced and analyzed. Results Three Banna viruses were isolated from mosquitoes collected in northwestern part of Yunnan during 2005 and 2006. Electron microscopy study showed that they are spherical with a diameter of 70 nm, no envelope but two layers of eapsid. It was found that the genome of the 3 isolates composes of 12 segments presenting band profile of 6-6 in RNA-PAGE. Nueleotide acid se-quence analysis about segment 12 showed that the identity was 99% between the 3 new isolates, 98% and 90% between the 3 isolates and the strains isolated in other parts of Yunnan, China and Indonesia, respec-tively. Phylogenetie analysis based on segment 12 gene showed that 3 new isolates clnstered in the same branch with the viruses isolated in other parts of Yunnan. The same difference of amino acids was found between Banna viruses isolated in China and Indonesia strains in the analysis of segment 12. Conclusion Banna virus strains were firstly isolated from mosquitoes collected in northwestern part of Yunnan province. Nueleotide acid sequence analysis of the 3 new isolates showed higher identity with strains isolated in other parts of Yunnan.
