CAJ | 학술논문

采用ISSR标记技术,对12种五针松的亲缘关系进行分析.利用筛选出的12个ISSR引物共检测到117个位点,多态条带比率(PPB)在9.40%～33.33 %之间,遗传分化最高的是偃松,最低的是柔枝松.12种五针松的基因多样性(Ht)为26.21%,其中种内基因多样性(Hs)为7.66%,种间基因多样性(Dst)为18.55%,五针松种间变异占总变异的70.78%.遗传距离聚类,将12种五针松分为2个类群,第一类群包括乔松、华山松、海南五针松、华南五针松、北美乔松、山白松和云南五针松;第二类群包括美国白皮松、偃松、、柔枝松、西伯利亚红松和红松.图1表3参6.
채용ISSR표기기술,대12충오침송적친연관계진행분석.이용사선출적12개ISSR인물공검측도117개위점,다태조대비솔(PPB)재9.40%～33.33 %지간,유전분화최고적시언송,최저적시유지송.12충오침송적기인다양성(Ht)위26.21%,기중충내기인다양성(Hs)위7.66%,충간기인다양성(Dst)위18.55%,오침송충간변이점총변이적70.78%.유전거리취류,장12충오침송분위2개류군,제일류군포괄교송、화산송、해남오침송、화남오침송、북미교송、산백송화운남오침송;제이류군포괄미국백피송、언송、、유지송、서백리아홍송화홍송.도1표3삼6.
Genetic relationship of 12 species of Section Strobus was analyzed with ISSR markers. 117 loci were detected with 12 ISSR primers. Percentage of polymorphic bands (PPB) varied from 5.93% to 19.92%. P. pumila had the highest levels of genetic differentiation and P. flexilis had lowest. Total genetic diversity (HT) of 12 species in Section Strobus was 26.21%, of which intraspecific genetic diversity (HS) was 7.66%, and interspecific genetic diversity (DST) was 18.55%, and the genetic variation in interspecies accounted for 70.78% of the total genetic diversity. According to the cluster results of genetic distance, the 12 species were classified into two groups. The first group included P. griffithii, P. armandi, P. fenzeliana, P. kwangtungensis, P. strobus, P. monticola and P. wangii. The second group included P. albicaulis, P. pumila, P. flexilis, P. sibirica and P. koraiensis.