CAJ | 학술논문

通过测定中国产罗布红麻、大叶白麻和白麻的ITS、trnL内含子和trnL-F非编码区序列,并与其它相关植物的相应序列比较,探讨"罗布麻"的系统分类.结果显示:罗布红麻、大叶白麻和白麻3种植物的ITS序列完全一致,而与罗布麻属加拿大麻的ITS序列相差较大;在trnL内含子和trnL-F非编码区,大叶白麻和白麻的完全一致,与罗布红麻之间仅有3个位点的变异,而加拿大麻与美国茶叶花在这3个位点却和白麻属的完全一致等.以上结果显示在ITS、trnL内含子和trnL-F非编码区,白麻属和罗布麻属之间没有本质区别,罗布红麻与大叶白麻和白麻之间的亲缘关系可能较罗布红麻与罗布麻属内其它物种间的亲缘关系更近.遗传距离与系统树的分析结果进一步支持以上推论.建议撤销白麻属,将大叶白麻和白麻合并到罗布麻属.
통과측정중국산라포홍마、대협백마화백마적ITS、trnL내함자화trnL-F비편마구서렬,병여기타상관식물적상응서렬비교,탐토"라포마"적계통분류.결과현시:라포홍마、대협백마화백마3충식물적ITS서렬완전일치,이여라포마속가나대마적ITS서렬상차교대;재trnL내함자화trnL-F비편마구,대협백마화백마적완전일치,여라포홍마지간부유3개위점적변이,이가나대마여미국다협화재저3개위점각화백마속적완전일치등.이상결과현시재ITS、trnL내함자화trnL-F비편마구,백마속화라포마속지간몰유본질구별,라포홍마여대협백마화백마지간적친연관계가능교라포홍마여라포마속내기타물충간적친연관계경근.유전거리여계통수적분석결과진일보지지이상추론.건의철소백마속,장대협백마화백마합병도라포마속.
To study the taxonomy of 'Luobuma' ,3 non-coding regions of DNA sequences (nuclear ribosomal ITS,chloroplast trnL intron, and trnL-F region) of Apocynum venetum, Poacynum pictum and P.hendersonii from China were sequenced and analyzed together with other related species from GenBank.ITS sequences from A. venetum, P. pictum and P. hendersonii were all the same and differed a lot from those of A. cannabinum. TrnL intron and trnL-F region sequences from P. pictum and P. hendersonii were the same;they differed from those of A. venetum at 3 sites,but were identical at these 3 sites to sequences from A. cannabinum and A. androsaemifolium. These results demonstrated that there is no intrinsic difference between Apocynum L. and Poacynum L. and the relationship between A. venetum and species from Poacynum L. is closer than that between A. venetum and other species from Apocynum L. Analyses of genetic distances and phylogenetic trees further confirmed this deduction. All of above supported the view that P. pictum and P. hendersonii should be incorporated into the genus Apocynum L. and the genus Poacynum L. should be repealed.