CAJ | 학술논문

利用杂交瘤技术制备了一株单克隆抗体T2-2,对其生化性质的研究及其与抗细胞角蛋白多克隆抗体完全重合的定位表明,该抗体特异性识别一种分子质量为46 ku的角蛋白.对68例正常组织和65例肿瘤组织的免疫组化结果显示,单克隆抗体T2-2具有上皮细胞特异性.与其他多数抗细胞角蛋白抗体常与一种以上细胞角蛋白多肽表现出交叉反应不同的是,该抗体只识别46 ku细胞角蛋白多肽上的某一单特异性表位.另外,单克隆抗体T2-2适用于多种免疫实验技术,包括ELISA、免疫组化、细胞免疫荧光及蛋白质印迹等,而且适用于多种固定剂.以上结果表明,单克隆抗体T2-2将成为细胞角蛋白功能研究和肿瘤诊断的有力工具.
이용잡교류기술제비료일주단극륭항체T2-2,대기생화성질적연구급기여항세포각단백다극륭항체완전중합적정위표명,해항체특이성식별일충분자질량위46 ku적각단백.대68례정상조직화65례종류조직적면역조화결과현시,단극륭항체T2-2구유상피세포특이성.여기타다수항세포각단백항체상여일충이상세포각단백다태표현출교차반응불동적시,해항체지식별46 ku세포각단백다태상적모일단특이성표위.령외,단극륭항체T2-2괄용우다충면역실험기술,포괄ELISA、면역조화、세포면역형광급단백질인적등,이차괄용우다충고정제.이상결과표명,단극륭항체T2-2장성위세포각단백공능연구화종류진단적유력공구.
A mAb T2-2 was generated using hybridoma techniques, and its target was identified as a 46 ku-cytokeratin (CK), based on biochemical study and a completely overlapped binding pattern of mAb T2-2 with anti-pan-CKs antibodies. An epithelia-specificity of the mAb T2-2 was determined by screening 68 human normal and 65 tumor tissues using immunohistochemistry. Unlike most of anti-CKs antibodies, the mAb T2-2 recognized a mono-specific epitope only expressed on the 46 ku CK, suggesting that mAb T2-2 is superior to most anti-CKs antibodies that cross-reacted with many different kinds of CKs. In addition, it was found that the mAb T2-2 was multipurpose with a broad applicability to ELISA, immunohistochemistry, immunofluorescence, Western blotting, and was also compatible with various fixation reagents. These results strongly indicate that the mAb T2-2 has potential applications for studying CKs function and for diagnosis of tumor and other disorders.