厦门大学学报(自然科学版)
廈門大學學報(自然科學版)
하문대학학보(자연과학판)
JOURNAL OF XIAMEN UNIVERSITY (NATURAL SCIENCE)
2009年
4期
576-580
,共5页
韩鹏%邱乒乒%李华亮%戴燕燕%李文岗%陈清西
韓鵬%邱乒乒%李華亮%戴燕燕%李文崗%陳清西
한붕%구핑핑%리화량%대연연%리문강%진청서
河蚬%抗氧化%胆管癌%增殖抑制%凋亡
河蜆%抗氧化%膽管癌%增殖抑製%凋亡
하현%항양화%담관암%증식억제%조망
Corbicula fluminea%antioxidation%cholangiocarcinoma%proliferation inhibit%apoptosis
作为传统中药的河蚬具有退热、治疗肝病和解酒等功效.以新鲜河蚬 (Corbicula fluminea)肉为原料,经甲醇抽提,乙酸乙酯萃取等方法得到河蚬乙酸乙酯提取物.运用DPPH法检测其清除自由基的能力,通过MTT法和倒置显微镜观察其对人胆管癌细胞QBC939生长的影响,并通过流式细胞术分析其对QBC939细胞周期的影响.结果显示:河蚬乙酸乙酯提取物对DPPH自由基具有一定的清除作用,在较低的浓度下有较高的清除效果,IC50值为0.6 mg/mL,且随其浓度的增加而不断增强;河蚬乙酸乙酯提取物对QBC939细胞有显著的抑制作用,处理48 h,IC50值为250 μg/mL,细胞形态发生明显变化;处理后的QBC939细胞出现明显的凋亡峰.研究表明:河蚬乙酸乙酯提取物不但具有抗氧化活性,且对体外培养的QBC939细胞具有明显的增殖抑制作用,能够改变QBC939细胞周期分布,并诱导QBC939细胞发生凋亡.说明河蚬乙酸乙酯提取物可能具有天然药物开发的潜力,同时也为胆管癌的防治提供新的思路和方法.
作為傳統中藥的河蜆具有退熱、治療肝病和解酒等功效.以新鮮河蜆 (Corbicula fluminea)肉為原料,經甲醇抽提,乙痠乙酯萃取等方法得到河蜆乙痠乙酯提取物.運用DPPH法檢測其清除自由基的能力,通過MTT法和倒置顯微鏡觀察其對人膽管癌細胞QBC939生長的影響,併通過流式細胞術分析其對QBC939細胞週期的影響.結果顯示:河蜆乙痠乙酯提取物對DPPH自由基具有一定的清除作用,在較低的濃度下有較高的清除效果,IC50值為0.6 mg/mL,且隨其濃度的增加而不斷增彊;河蜆乙痠乙酯提取物對QBC939細胞有顯著的抑製作用,處理48 h,IC50值為250 μg/mL,細胞形態髮生明顯變化;處理後的QBC939細胞齣現明顯的凋亡峰.研究錶明:河蜆乙痠乙酯提取物不但具有抗氧化活性,且對體外培養的QBC939細胞具有明顯的增殖抑製作用,能夠改變QBC939細胞週期分佈,併誘導QBC939細胞髮生凋亡.說明河蜆乙痠乙酯提取物可能具有天然藥物開髮的潛力,同時也為膽管癌的防治提供新的思路和方法.
작위전통중약적하현구유퇴열、치료간병화해주등공효.이신선하현 (Corbicula fluminea)육위원료,경갑순추제,을산을지췌취등방법득도하현을산을지제취물.운용DPPH법검측기청제자유기적능력,통과MTT법화도치현미경관찰기대인담관암세포QBC939생장적영향,병통과류식세포술분석기대QBC939세포주기적영향.결과현시:하현을산을지제취물대DPPH자유기구유일정적청제작용,재교저적농도하유교고적청제효과,IC50치위0.6 mg/mL,차수기농도적증가이불단증강;하현을산을지제취물대QBC939세포유현저적억제작용,처리48 h,IC50치위250 μg/mL,세포형태발생명현변화;처리후적QBC939세포출현명현적조망봉.연구표명:하현을산을지제취물불단구유항양화활성,차대체외배양적QBC939세포구유명현적증식억제작용,능구개변QBC939세포주기분포,병유도QBC939세포발생조망.설명하현을산을지제취물가능구유천연약물개발적잠력,동시야위담관암적방치제공신적사로화방법.
As a traditional chinese herb, Corbicula fluminea has many effects, such as, defervescence, curing the liver ailment, antialcoholism, and so on. We used fresh Corbicula fluminea body as the material and obtained the ethyl acetate extract by the method of ethyl acetate extraction. The ability of antioxidation was measured by DPPH method. The inhibition rate of cholangiocarcinoma cells was measured by MTT method. The morphological change of QBC939 cells was detected by ordinary inverted microscopy. The effect on cell cycles of QBC939 was observed by flow cytometry (FCM) assay. The results showed that ethyl acetate extract from Corbicula fluminea has strong ability to scavenging up DPPH radical, of which the value of IC50 was 0.6 mg/mL. And it could also inhibit the growth of QBC939 cells in a dose- and time-dependent manner, and the IC50 value was determined to be 250 μg/mL for 48 h treatment. The morphological of QBC939 cells changed significantly. At the same time, FCM showed that Corbicula fluminea ethyl acetate extract could change the cell cycle of QBC939 cells, and induce apoptosis. These results indicated that Corbicula fluminea ethyl acetate extract had the ability of antioxidation and effectively inhibited the proliferation of the human cholangiocarcinoma cell line QBC939 in vitro and acted as an apoptosis inducer in its antitumor effects via inducing morphological changes and cell cycle arrest. Thus, Corbicula fluminea ethyl acetate extract has the potential of becoming a natural drug, and it also provide a new thought and a new method for the therapy of cholangiocarcinoma.