CAJ | 학술논문

目的:探讨G_0S_2在调节鼠骨髓基质细胞向脂肪细胞分化过程中的作用.方法:利用原代培养小鼠骨髓基质细胞(Marrowstrom cell,MSC),脂质体转染法将表达质粒pCAG-PPARγ2及含报告基因的pGL_2-COL_1A质粒共转染至MSC,G418筛选,RT-PCR检测PPARγ及G0S2mRNA表达,免疫组化检测PPARγ的表达,westen blot检测G_0S_2的表达,油红O染色检测细胞内脂滴.结果:MSC在未分化状态下不表达G_0S_2,经PPARγ基因转染后,G_0S_2开始表达,转染细胞最终分化为脂肪细胞.结论:PPARγ通过调节G_0S_2启动子调节后者表达,进而调节MSC向脂肪细胞分化,G_0S_2基因参与调节MSC向脂肪细胞分化.
목적:탐토G_0S_2재조절서골수기질세포향지방세포분화과정중적작용.방법:이용원대배양소서골수기질세포(Marrowstrom cell,MSC),지질체전염법장표체질립pCAG-PPARγ2급함보고기인적pGL_2-COL_1A질립공전염지MSC,G418사선,RT-PCR검측PPARγ급G0S2mRNA표체,면역조화검측PPARγ적표체,westen blot검측G_0S_2적표체,유홍O염색검측세포내지적.결과:MSC재미분화상태하불표체G_0S_2,경PPARγ기인전염후,G_0S_2개시표체,전염세포최종분화위지방세포.결론:PPARγ통과조절G_0S_2계동자조절후자표체,진이조절MSC향지방세포분화,G_0S_2기인삼여조절MSC향지방세포분화.
Objective: To explore the effects of G_0S_2 in the differentiation of mouse mallow stroma cells (MSCs) into adipocytes. Methods: The primary MSCs were cultured. The eukaryotic expression plasmid vector Pcag-PPAR γ 2 and Pgl_2-COL_1A reporter vector were cotransfected into MSCs with lipotransfection method followed by G418 selection. The expression levels of PPAR γ Mrna and G_0S_2 Mrna were detected by RT-PCR. Immunohistochemistry and Westen blot were emplored to study the protein expression of PPAR γ and G_0S_2. Lipid droplets in the cells were stained with Oil Red O. Results :No expression of G_0S_2 Mrna was found in undifferentiated MSCs. The G_0S_2 Mrna expressed in the MSCs after transfected with PCAG-PPARγ2, and the cells finaly differentiated into adipocytes. Conclusion:PPARγ stimulated G_0S_2 promoter activity via the PPRE in vivo.Then the expressions of G_0S_2 can regulate the adipocyte differentiaiton of MSCs.