安徽农业科学
安徽農業科學
안휘농업과학
JOURNAL OF ANHUI AGRICULTURAL SCIENCES
2010年
5期
2404-2405
,共2页
沈晓鹏%卞桂华%袁华根%罗有文%刘静%周伟伟
瀋曉鵬%卞桂華%袁華根%囉有文%劉靜%週偉偉
침효붕%변계화%원화근%라유문%류정%주위위
Alloferon1%犬细小病毒%抗病毒作用
Alloferon1%犬細小病毒%抗病毒作用
Alloferon1%견세소병독%항병독작용
Alloferon1%CPV%Anti-virus action
[目的]开发出一种用于防治犬细小病毒病的新药物.[方法]将不同剂量的Alloferon1添加于病毒感染前、病毒感染同时、病毒感染后的细胞培养液中进行体外抗犬细小病毒试验.[结果]FK81细胞接种CPV后2 d,细胞开始变圆,胞质内颗粒增多;4~6 d后病变细胞呈葡萄串状脱落.在80%细胞产生病变时收取病毒,TCID50为10_(-6.2)~10_(-6.3)/ml.对照组细胞上清的血凝反应为阴性,接种病毒细胞上清的血凝价为2~(12).对照组的8孔细胞生长正常,接种病毒的8孔细胞全部产生病变.250.0和25.0 μg/ml 2个浓度的抗病毒作用较强,2.5 μg/ml的抗病毒作用较弱.在病毒感染前或同时加入多肽的抗病毒作用较好,而在病毒感染后加入多肽的抗病毒作用较弱.[结论]Alloferon1可以用于犬细小病毒病的防治.
[目的]開髮齣一種用于防治犬細小病毒病的新藥物.[方法]將不同劑量的Alloferon1添加于病毒感染前、病毒感染同時、病毒感染後的細胞培養液中進行體外抗犬細小病毒試驗.[結果]FK81細胞接種CPV後2 d,細胞開始變圓,胞質內顆粒增多;4~6 d後病變細胞呈葡萄串狀脫落.在80%細胞產生病變時收取病毒,TCID50為10_(-6.2)~10_(-6.3)/ml.對照組細胞上清的血凝反應為陰性,接種病毒細胞上清的血凝價為2~(12).對照組的8孔細胞生長正常,接種病毒的8孔細胞全部產生病變.250.0和25.0 μg/ml 2箇濃度的抗病毒作用較彊,2.5 μg/ml的抗病毒作用較弱.在病毒感染前或同時加入多肽的抗病毒作用較好,而在病毒感染後加入多肽的抗病毒作用較弱.[結論]Alloferon1可以用于犬細小病毒病的防治.
[목적]개발출일충용우방치견세소병독병적신약물.[방법]장불동제량적Alloferon1첨가우병독감염전、병독감염동시、병독감염후적세포배양액중진행체외항견세소병독시험.[결과]FK81세포접충CPV후2 d,세포개시변원,포질내과립증다;4~6 d후병변세포정포도천상탈락.재80%세포산생병변시수취병독,TCID50위10_(-6.2)~10_(-6.3)/ml.대조조세포상청적혈응반응위음성,접충병독세포상청적혈응개위2~(12).대조조적8공세포생장정상,접충병독적8공세포전부산생병변.250.0화25.0 μg/ml 2개농도적항병독작용교강,2.5 μg/ml적항병독작용교약.재병독감염전혹동시가입다태적항병독작용교호,이재병독감염후가입다태적항병독작용교약.[결론]Alloferon1가이용우견세소병독병적방치.
[Objective] The research aimed to develop a new drug which could be used to control Canine parvovirus (CPV).[Method] Different doses ofAlloferon1 were added into cell culture fluid before,when and after the cells were infected with viruses for performing experiment on in vitro anti-CPA.[Result] In 2 d since FK81 cells were inoculated with CPV,the cells was becoming round and the particles were increasing in cytoplast and in the period from the 4th day to the 6th day since that time,the diseased cells fell off in thyrsiform.The viruses were yielded when 80% cells were diseased and the TCID50 was 10_(-6.2)-10_(-6.3)/ml.The haemagglutination of supernatant of cells in control group was negative and the haemagglutinating value of supernatant of cells inoculated with viruses was 2~(12).The cells in the 8 holes of control group grew normally and that in the other 8 holes inoculated with viruses were all diseased.The 2 concn.of 250.0 and 25.0 μg/ml were stronger and that of 2.5 μg/ml was weaker.The antivirus actions of adding polypeptides before and when the cells were infected with viruses were better and that of adding polypeptides,after the cells were infected with viruses,was weaker.[Conclusion] Alloferon1 could be used to control CPV.