CAJ | 학술논문

目的通过改良接种方式建立兔肝癌模型,分析并观察该肿瘤的生长特性.方法经肝包膜植入瘤组织块(约106～108个瘤细胞)接种于20只兔的肝左叶,建立兔肝癌模型.观察:①肿瘤在实验兔中生长7、10、14、17和21 d时的体积(B超测),并计算肿瘤生长率.②大体及光镜下观察瘤组织形态特征.结果瘤体呈指数性生长,在接种17 d后生长迅速.组织病理表明该瘤在肝组织中浸润式生长,肿瘤体积增长快于直径增长,其性状与VX2鳞状细胞癌特征相似.结论瘤块种植是建立兔肝癌模型的首选方式,该模型是肝癌的基础及临床的理想动物模型.
목적 통과개량접충방식건립토간암모형,분석병관찰해종류적생장특성.방법 경간포막식입류조직괴(약106～108개류세포)접충우20지토적간좌협,건립토간암모형.관찰:①종류재실험토중생장7、10、14、17화21 d시적체적(B초측),병계산종류생장솔.②대체급광경하관찰류조직형태특정.결과 류체정지수성생장,재접충17 d후생장신속.조직병리표명해류재간조직중침윤식생장,종류체적증장쾌우직경증장,기성상여VX2린상세포암특정상사.결론 류괴충식시건립토간암모형적수선방식,해모형시간암적기출급림상적이상동물모형.
Objective To establish a rabbit VX2 liver tumor model by transplanting tumor tissue mass into the rabbit's liver,to analyze and observe the growing features of the liver tumor.Methods The tumor tissue mass(about 106-108 VX2 liver tumor cells)was inoculated into the left hepatic labe in 20 rabbits to establish rabbit VX-2 hepatic carcinoma model.The observation included the following two respects.(1)The tumor's volume at 7,10,14,17 and 21 days after the procedure was measured by ultrasonography and the growth rate of tumor was calculated.(2)The morphological feature of the tumor was inspected both macroscopically and microscopically.Results The growing pattern of the tumor was compatible with the exponential curve.Seventeen days after transplantation the increase rate of the tumor volume was much higher than that of the tumor diameter. Histopathologjcally,the growing pattern of the tumor took the form of infiltrative way,with its appearance being quite similar to the VX2 squamous cell carcinoma.Conclusion Transplantation of tumor tissue mass is the technique of first choice to establish the VX2 liver carcinoma model in rabbits.This experimental model is a very ideal animal form for both clinical and fundamental studies of liver carcinoma.