中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2009年
2期
138-141
,共4页
张丽薇%高彦辉%耿利彬%高琳%孙殿军
張麗薇%高彥輝%耿利彬%高琳%孫殿軍
장려미%고언휘%경리빈%고림%손전군
氟%铝%基质金属蛋白酶13%关节%软骨细胞
氟%鋁%基質金屬蛋白酶13%關節%軟骨細胞
불%려%기질금속단백매13%관절%연골세포
Fluorine%Aluminum%Matrix metalloproteinase 13%Joints%Chondrocytes
目的 观察氟、铝对大鼠关节软骨细胞基质金属蛋白酶-13(MMP-13)表达的影响.方法 培养大鼠原代软骨细胞,分为加氟组、加铝组、氟+铝组和对照组,分别应用终浓度为1 mmol/L的NaF和2 mmol/L的A1C13染毒24、48、72 h,在不同时间提取细胞,用反转录聚合酶链反应(RT-PCR)检测MMP-13 mRNA表达,并用Western-blot方法检测其蛋白表达.结果 染毒24 h,加氟组(0.830±0.043)、加铝组(1.279±0.060)、氟+铝组(0.983±0.028)MMP-13 mRNA水平均高于对照组(0.707±0.026,P<0.05),其中加铝组相对表达量最高;染毒48 h,加氟组(0.964±0.180)、加铝组(1.333±0.105)、氟+铝组(0.915±0.137)MMP-13 mRNA水平均高于对照组(0.660±0.055,P<0.05),加铝组相对表达量仍最高;染毒72 h,加氟组(0.866±0.115)、加铝组(0.846±0.089)、氟+铝组(0.967±0.196)MMP-13 mRNA水平与对照组(0.809±0.179)比较,差异无统计学意义(P>0.05).染毒24 h,加氟组(1.050±0.084)、加销组(1.010±0.113)、氟+铝组(0.977±0.202)MMP-13蛋白水平与对照组(0.860±0.038)比较,差异无统计学意义(P>0.05);染毒48 h,加氟组(0.671±0.020)、加铝组(1.134±0.094)、氟+铝组(0.923±0.087)MMP-13蛋白水平均高于对照组(0.647±0.025,P<0.05),但各实验组之间比较差异无统计学意义(P>0.05);染毒72 h,加氟组(0.672±0.022)、加铝组(1.088±0.072)、氟+铝组(0.772±0.030)MMP-13蛋白水平均高于对照组(0.577±0.026,P<0.05),其中加铝组最高,同加氟组和氟+铝组比较,差异均有统计学意义(P<0.05).结论 氟、铝对软骨细胞有一定的损伤作用,铝单独作用的毒性要大于氟和氟+铝组,氟、铝致软骨细胞损伤过程中伴MMP-13表达异常.
目的 觀察氟、鋁對大鼠關節軟骨細胞基質金屬蛋白酶-13(MMP-13)錶達的影響.方法 培養大鼠原代軟骨細胞,分為加氟組、加鋁組、氟+鋁組和對照組,分彆應用終濃度為1 mmol/L的NaF和2 mmol/L的A1C13染毒24、48、72 h,在不同時間提取細胞,用反轉錄聚閤酶鏈反應(RT-PCR)檢測MMP-13 mRNA錶達,併用Western-blot方法檢測其蛋白錶達.結果 染毒24 h,加氟組(0.830±0.043)、加鋁組(1.279±0.060)、氟+鋁組(0.983±0.028)MMP-13 mRNA水平均高于對照組(0.707±0.026,P<0.05),其中加鋁組相對錶達量最高;染毒48 h,加氟組(0.964±0.180)、加鋁組(1.333±0.105)、氟+鋁組(0.915±0.137)MMP-13 mRNA水平均高于對照組(0.660±0.055,P<0.05),加鋁組相對錶達量仍最高;染毒72 h,加氟組(0.866±0.115)、加鋁組(0.846±0.089)、氟+鋁組(0.967±0.196)MMP-13 mRNA水平與對照組(0.809±0.179)比較,差異無統計學意義(P>0.05).染毒24 h,加氟組(1.050±0.084)、加銷組(1.010±0.113)、氟+鋁組(0.977±0.202)MMP-13蛋白水平與對照組(0.860±0.038)比較,差異無統計學意義(P>0.05);染毒48 h,加氟組(0.671±0.020)、加鋁組(1.134±0.094)、氟+鋁組(0.923±0.087)MMP-13蛋白水平均高于對照組(0.647±0.025,P<0.05),但各實驗組之間比較差異無統計學意義(P>0.05);染毒72 h,加氟組(0.672±0.022)、加鋁組(1.088±0.072)、氟+鋁組(0.772±0.030)MMP-13蛋白水平均高于對照組(0.577±0.026,P<0.05),其中加鋁組最高,同加氟組和氟+鋁組比較,差異均有統計學意義(P<0.05).結論 氟、鋁對軟骨細胞有一定的損傷作用,鋁單獨作用的毒性要大于氟和氟+鋁組,氟、鋁緻軟骨細胞損傷過程中伴MMP-13錶達異常.
목적 관찰불、려대대서관절연골세포기질금속단백매-13(MMP-13)표체적영향.방법 배양대서원대연골세포,분위가불조、가려조、불+려조화대조조,분별응용종농도위1 mmol/L적NaF화2 mmol/L적A1C13염독24、48、72 h,재불동시간제취세포,용반전록취합매련반응(RT-PCR)검측MMP-13 mRNA표체,병용Western-blot방법검측기단백표체.결과 염독24 h,가불조(0.830±0.043)、가려조(1.279±0.060)、불+려조(0.983±0.028)MMP-13 mRNA수평균고우대조조(0.707±0.026,P<0.05),기중가려조상대표체량최고;염독48 h,가불조(0.964±0.180)、가려조(1.333±0.105)、불+려조(0.915±0.137)MMP-13 mRNA수평균고우대조조(0.660±0.055,P<0.05),가려조상대표체량잉최고;염독72 h,가불조(0.866±0.115)、가려조(0.846±0.089)、불+려조(0.967±0.196)MMP-13 mRNA수평여대조조(0.809±0.179)비교,차이무통계학의의(P>0.05).염독24 h,가불조(1.050±0.084)、가소조(1.010±0.113)、불+려조(0.977±0.202)MMP-13단백수평여대조조(0.860±0.038)비교,차이무통계학의의(P>0.05);염독48 h,가불조(0.671±0.020)、가려조(1.134±0.094)、불+려조(0.923±0.087)MMP-13단백수평균고우대조조(0.647±0.025,P<0.05),단각실험조지간비교차이무통계학의의(P>0.05);염독72 h,가불조(0.672±0.022)、가려조(1.088±0.072)、불+려조(0.772±0.030)MMP-13단백수평균고우대조조(0.577±0.026,P<0.05),기중가려조최고,동가불조화불+려조비교,차이균유통계학의의(P<0.05).결론 불、려대연골세포유일정적손상작용,려단독작용적독성요대우불화불+려조,불、려치연골세포손상과정중반MMP-13표체이상.
Objective To observe the influence of fluoride and aluminum on the expression of matrix metalloproteinase-13(MMP-13) in rat articular chondrocytes. Methods Original generation chondrocytes of rats was cultured and divided into fluoride group, aluminum group, fluoride plus aluminum group and control group. NaF and A1C13 at concentrations of 1 mmol/L and 2 mmol/L were administered to intoxicate the cells for 24, 48, 72 h respectively. Cells were extracted to undergo reverse transcription the polymerase chain reaction(RT-PCR) at different times to observe mRNA expression of MMP-13, and protein expression was detected by Western-blot. Results In 24 h, the content of MMP-13 mRNA in fluoride group(0.830±0.043), aluminum group(1.279±0.060) and fluoride plus aluminum group(0.983±0.028) was higher than that in the control group(0.707±0.026, P<0.05), and relative expression of MMP-13 mRNA in aluminum group was the highest. In 48 h, the content of MMP-13 mRNA in fluoride group (0.964±0.180), aluminum group (1.333±0.105) and fluoride plus aluminum group (0.915±0.137) was higher than that in the control group(0.660±0.055, P<0.05), and the relative expression in aluminum group was the highest. In 72 h, the content of MMP-13 mRNA in fluoride group(0.866±0.115), aluminum group(0.846±0.089) and fluoride plus aluminum group(0.967±0.196) had no statistical significance(P>0.05) compared with the control group(0.809±0.179). In 24 h, the content of MMP-13 protein in fluoride group(1.050±0.084), aluminum group(1.010±0.113) and fluoride plus aluminum group(0.977±0.202) had no statistical significance(P>0.05) compared with the control group(0.860±0.038). In 48 h, the content of MMP-13 protein in fluoride group(0.671±0.020), aluminum group(1.134±0.094) and fluoride plus aluminum group (0.923±0.087) was higher than that in the control group (0.647±0.025, P<0.05), but no significant difference being observed between groups (P>0.05). In 72 h, the content of MMP-13 protein in fluoride group(0.672±0.022), aluminum group(1.088±0.072) and fluoride plus aluminum group(0.772±0.030) was higher than that in the control group(0.577±0.026, P<0.05). It was the highest in the aluminum group, the intra-group difference had statistical significance(P<0.05). Conclusions Fluoride and aluminum damage chondrocytes to some extent, toxicity of aluminum itself is greater than fluoride and fluoride plus aluminum. Abnormal expression of MMP-13 can be observed in the chondrocyte damage process induced by fluoride and aluminum.
