CAJ | 학술논문

目的观察土牛膝的急性毒性反应,了解其对主要脏器的损伤情况.方法本实验分两步,预试验初步确定用药剂量,正式实验采用40只小鼠,随机分为空白对照组和4个剂量组分别为400g/kg、300g/kg、225 g/kg、169 g/kg,连续灌胃7 d,观察小鼠症状表现及死亡情况,通过统计学处理得出半数致死量(LD50).并对死亡小鼠进行解剖,观察器官的损伤情况.结果小鼠的LD50为309.21 g/kg、标准差(Sx)=0.0359 g/kg,LD50的95%可信限为309.14～309.28 g/kg,按毒性分级标准,土牛膝为无毒类物.病理切片显示,大剂量给药后小鼠肝、脾组织均有一定程度的淤血.结论土牛膝毒性较小,大剂量给药对小鼠心脏有一定影响.
목적 관찰토우슬적급성독성반응,료해기대주요장기적손상정황.방법 본실험분량보,예시험초보학정용약제량,정식실험채용40지소서,수궤분위공백대조조화4개제량조분별위400g/kg、300g/kg、225 g/kg、169 g/kg,련속관위7 d,관찰소서증상표현급사망정황,통과통계학처리득출반수치사량(LD50).병대사망소서진행해부,관찰기관적손상정황.결과 소서적LD50위309.21 g/kg、표준차(Sx)=0.0359 g/kg,LD50적95%가신한위309.14～309.28 g/kg,안독성분급표준,토우슬위무독류물.병리절편현시,대제량급약후소서간、비조직균유일정정도적어혈.결론 토우슬독성교소,대제량급약대소서심장유일정영향.
Objective To observe the acute toxicity of local achyranthes aspera, and its injury to major internal organs. Methods Estimated the dose before the experiment, then selected 40 mice and divided them into five groups randomly, namely 4 dose-groups and one control group. The doses used in the dose-groups were 400 g/kg, 300 g/kg, 225 g/kg and 169 g/kg respectively. Observed the symptoms and the death for successive 7 days and calculated LD (50)with statistic methods. Dissected the dead mice and observed the lesion of organs. Results The LD50 of acute toxicity test of achyranthes aspera was 309.21 g/kg, Sx=0.0359 g/kg. 95% of the limit of trust was 309.14～309.28 g/kg. According to the acute toxic standard, it belonged to non-toxicity. The specimen revealed that large dose administration caused coagulation of blood in both liver and spleen. Conclusion Local achyranthes aspera had little toxicity. Large dose administration affected heart function of mice.