CAJ | 학술논문

目的探讨康艾注射液对人结直肠癌Lovo细胞Th1/Th2状态的影响.方法常规培养Lovo细胞,待细胞处于指数增长期再分成4组:1组为空白对照,另外3组加入不同浓度的康艾注射液.培养72h后,采用双抗夹心酶联免疫吸附法(ELISA)分别检测各组Lovo细胞培养上清中Th1类细胞因子(IL-2,IFN-γ)和Th2类细胞因子(IL-4,IL-10)的水平.结果 Lovo细胞与康艾注射液共培养后,上清液中Th2类细胞因子的含量较对照组明显降低(P＜0.05),而Th1类细胞因子在各组中的含量变化不大.结论康艾注射液可显著下调Lovo细胞Th2类细胞因子水平,具有促进其Th2向Th1逆转的倾向.
목적 탐토강애주사액대인결직장암Lovo세포Th1/Th2상태적영향.방법 상규배양Lovo세포,대세포처우지수증장기재분성4조:1조위공백대조,령외3조가입불동농도적강애주사액.배양72h후,채용쌍항협심매련면역흡부법(ELISA)분별검측각조Lovo세포배양상청중Th1류세포인자(IL-2,IFN-γ)화Th2류세포인자(IL-4,IL-10)적수평.결과 Lovo세포여강애주사액공배양후,상청액중Th2류세포인자적함량교대조조명현강저(P＜0.05),이Th1류세포인자재각조중적함량변화불대.결론강애주사액가현저하조Lovo세포Th2류세포인자수평,구유촉진기Th2향Th1역전적경향.
Objective To explore the effect of Kang'ai injection on Th1/Th2 cytokine in human colon carcinoma cell line Lovo.Methods Lovo cells were cultured by conventional ways until they were in exponential growth phase and then divided into 4 groups:one control group and three experimental groups with different concentration of Kang'ai injection.After 72 hours culture,Th1 type cytokines (IL-2,IFN-γ) and Th2.type cytokines (IL-4,IL-10) in the culture supernatant of Lovo cells in each group were separately detected by double-antibody sandwich enzyme-linked immunosorhent assay (ELISA).Results After the coculture of Lovo cells and Kang'ai injection,Th2 type cytokine levels in the supernatants were significant lower than the control group (P＜0.05),and Th1 type cytokines in each group were of little change.Conclusions Kang'ai injection markedly downregulated Th2 type cytokines in Lovo cells,and contributed to the switching from Th2 to Th1.