中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2011年
7期
506-509
,共4页
方国峰%范雪云%柳建强%沈福海%晏丽
方國峰%範雪雲%柳建彊%瀋福海%晏麗
방국봉%범설운%류건강%침복해%안려
矽肺%白细胞介素-4%多态性,单核苷酸%疾病易感性
矽肺%白細胞介素-4%多態性,單覈苷痠%疾病易感性
석폐%백세포개소-4%다태성,단핵감산%질병역감성
Silicosis%Interleukin-4%Polymorphism,single nucleotide%Disease susceptibility
目的 探讨白细胞介素-4(IL-4)(-33、+45、VNTR、+429、+448)位点基因多态性与矽肺易感性的关系.方法 采用整群病例对照研究,病例组为101名Ⅰ期矽肺患者,对照组为与病例年龄相近、同性别、同民族、同一工作地点、累积接尘工龄相近的非矽肺接尘工人121名.采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法检测IL-4的5个基因位点的基因型并进行分析.结果 两组对象的年龄、累积接尘工龄、吸烟率比较,差异均无统计学意义(P＞0.05).IL-4(+429)位点仅存在GA 基因型,未发现GG和AA基因型;IL-4(+448)位点仅存在CC基因型,未发现CG和GG基因型.病例组IL-4(+45)位点AA、GG和AG基因型频率分别为55.4%、10.9%和33.7%,与对照组(62.0%、11.6%、26.4%)比较,差异无统计学意义(x2=1.386,P＞0.05);病例组IL-4(VNTR)位点B1B1、B282和B182基因型频率分别为73.3%、1.0%和25.7%,与对照组(68.6%、1.7%、29.8%)比较,差异无统计学意义(x2=0.580,P＞0.05);病例组IL-4(-33)位点TY、CC和CT基因型频率分别为55.4%、11.9%和32.7%,与对照组(69.4%、4.1%、26.4%)比较,差异有统计学意义(x2=6.751,P＜0.05).结论 未发现IL-4(+45、VNTR)与矽肺患病的关系,未发现IL-4(+448、+429)存在多态性现象.IL-4(-33)位点多态性与矽肺患病有关,携带IL-4-33等位基因C的接尘者患矽肺的危险性增加.
目的 探討白細胞介素-4(IL-4)(-33、+45、VNTR、+429、+448)位點基因多態性與矽肺易感性的關繫.方法 採用整群病例對照研究,病例組為101名Ⅰ期矽肺患者,對照組為與病例年齡相近、同性彆、同民族、同一工作地點、纍積接塵工齡相近的非矽肺接塵工人121名.採用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)法檢測IL-4的5箇基因位點的基因型併進行分析.結果 兩組對象的年齡、纍積接塵工齡、吸煙率比較,差異均無統計學意義(P＞0.05).IL-4(+429)位點僅存在GA 基因型,未髮現GG和AA基因型;IL-4(+448)位點僅存在CC基因型,未髮現CG和GG基因型.病例組IL-4(+45)位點AA、GG和AG基因型頻率分彆為55.4%、10.9%和33.7%,與對照組(62.0%、11.6%、26.4%)比較,差異無統計學意義(x2=1.386,P＞0.05);病例組IL-4(VNTR)位點B1B1、B282和B182基因型頻率分彆為73.3%、1.0%和25.7%,與對照組(68.6%、1.7%、29.8%)比較,差異無統計學意義(x2=0.580,P＞0.05);病例組IL-4(-33)位點TY、CC和CT基因型頻率分彆為55.4%、11.9%和32.7%,與對照組(69.4%、4.1%、26.4%)比較,差異有統計學意義(x2=6.751,P＜0.05).結論 未髮現IL-4(+45、VNTR)與矽肺患病的關繫,未髮現IL-4(+448、+429)存在多態性現象.IL-4(-33)位點多態性與矽肺患病有關,攜帶IL-4-33等位基因C的接塵者患矽肺的危險性增加.
목적 탐토백세포개소-4(IL-4)(-33、+45、VNTR、+429、+448)위점기인다태성여석폐역감성적관계.방법 채용정군병례대조연구,병례조위101명Ⅰ기석폐환자,대조조위여병례년령상근、동성별、동민족、동일공작지점、루적접진공령상근적비석폐접진공인121명.채용취합매련반응-한제성편단장도다태성(PCR-RFLP)법검측IL-4적5개기인위점적기인형병진행분석.결과 량조대상적년령、루적접진공령、흡연솔비교,차이균무통계학의의(P＞0.05).IL-4(+429)위점부존재GA 기인형,미발현GG화AA기인형;IL-4(+448)위점부존재CC기인형,미발현CG화GG기인형.병례조IL-4(+45)위점AA、GG화AG기인형빈솔분별위55.4%、10.9%화33.7%,여대조조(62.0%、11.6%、26.4%)비교,차이무통계학의의(x2=1.386,P＞0.05);병례조IL-4(VNTR)위점B1B1、B282화B182기인형빈솔분별위73.3%、1.0%화25.7%,여대조조(68.6%、1.7%、29.8%)비교,차이무통계학의의(x2=0.580,P＞0.05);병례조IL-4(-33)위점TY、CC화CT기인형빈솔분별위55.4%、11.9%화32.7%,여대조조(69.4%、4.1%、26.4%)비교,차이유통계학의의(x2=6.751,P＜0.05).결론 미발현IL-4(+45、VNTR)여석폐환병적관계,미발현IL-4(+448、+429)존재다태성현상.IL-4(-33)위점다태성여석폐환병유관,휴대IL-4-33등위기인C적접진자환석폐적위험성증가.
Objective To explore the relationship between polymorphisms of interleukin-4 (IL-4)gene (-33,+45,VNTR,+429,+448) and the susceptibility of silicosis. Methods In a case-control study, the case group consisted of 101 patients with silicosis, and was matched with the control group (121 workers without silicosis), according to the age, sex, nationality, working place, exposure to dust. The polymorphisms of IL-4(five locus) detected by the method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques. Results There was no difference of age, exposure and smoking between case group and control group (P＞0.05). The two groups had good comparability. Only the GA genotype in the IL-4 (+429)locus was found, the genotypes of AA and GG were not found. The CC genotype in the IL-4 (+448) locus was found, the genotypes of CG and GG were not found. The frequencies of AA, GG and AG of IL-4 (+45) locus in the case and control groups were 55.4%, 10.9%, 33.7% and 62.0%, 11.6%, 26.4%, respectively, there was no the significant difference between case and control groups (P＞0.05). The frequencies of B1B1, B2B2 and B1B2of IL-4 (VNTR) locus in the case and control groups were 73.3%, 1.0%, 25.7% and 68.6%, 1.7%, 29.8%,respectively, there was no the significant difference between case and control groups (P＞0.05). The frequencies of TT, CC and CT in IL-4 -33 locus in the case group were 55.4%, 11.9% and 32.7%, which were significantly higher than those (69.4%, 4.1%, 26.4%) in control group (P＜0.05). Conclusions There was no relationship between IL-4 (+45,VNTR) genotypes and prevalence of silicosis in this study. The polymorphisms of IL-4(+448)site were not found which may be related to the race. The relationship between genetic polymorphism of IL-4(-33) locus and silicosis development was found, Workers with IL-4(-33) allele C are susceptible to the silica.
