中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2012年
5期
406-409
,共4页
李照%张栋%陈建飞%苏万春%高杰%李涛%栗光明%冷希圣%朱继业
李照%張棟%陳建飛%囌萬春%高傑%李濤%慄光明%冷希聖%硃繼業
리조%장동%진건비%소만춘%고걸%리도%률광명%랭희골%주계업
肝炎疫苗,乙型%肝移植%动物实验%滤泡辅助T细胞
肝炎疫苗,乙型%肝移植%動物實驗%濾泡輔助T細胞
간염역묘,을형%간이식%동물실험%려포보조T세포
Hepatitis B,vaccines%Liver transplantation%Animal experimentation%Follicular helper T cell
目的 研究环孢素A(CsA)联合IL-2融合蛋白对小鼠移植术后乙肝疫苗的体液免疫反应影响,寻求肝移植术后预防乙型肝炎的有效方法.方法 将C57BL/6小鼠40只,分为实验组、对照组和空白组,未免疫组,每组10只.实验组、对照组行皮肤移植后(DBA小鼠为供体),实验组给予CsA(30 mg/kg,腹腔内注射)+IL-2融合蛋白,对照组只给予CsA,空白组只行皮肤移植,3组均给予乙肝疫苗安在时,未免疫组不作任何处理.14 d后测定各组乙肝抗体滴度水平、IL-4、IL-10、IL-2、IFN-γ水平,流式细胞测定各组淋巴组织中滤泡辅助T细胞(follicular helperT cell,Tfh)的比例及应用RT-PCR测定各组淋巴组织中IL-21和叉状头转录因子FoxP3的表达.结果 实验组移植术后14 d乙肝抗体滴度明显高于对照组(F=29.886,P=0.010),皮肤移植物的存活时间也明显长于对照组(F=29.772,P=0.011),ELISA测定血清Th2类因子IL-4,IL-10的水平实验组明显低于对照组(F=42.102,P=0.009;F=23.734,P-0.015),Th1类因子IL-2和IFN-γ水平明显高于对照组(F=18.156,P=0.0020;F =90.042,P =0.003).实验组混合淋巴细胞RT-PCR测定IL-21、FoxP3的表达水平明显高于对照组(F=9.784,P =0.048；F=27.883,P=0.012).结论 CsA联合IL-2融合蛋白能够增强乙肝疫苗的抗体滴度,同时能够延长小鼠皮肤移植物的存活时间,可能与促进IL-21及FoxP3基因的表达有关.
目的 研究環孢素A(CsA)聯閤IL-2融閤蛋白對小鼠移植術後乙肝疫苗的體液免疫反應影響,尋求肝移植術後預防乙型肝炎的有效方法.方法 將C57BL/6小鼠40隻,分為實驗組、對照組和空白組,未免疫組,每組10隻.實驗組、對照組行皮膚移植後(DBA小鼠為供體),實驗組給予CsA(30 mg/kg,腹腔內註射)+IL-2融閤蛋白,對照組隻給予CsA,空白組隻行皮膚移植,3組均給予乙肝疫苗安在時,未免疫組不作任何處理.14 d後測定各組乙肝抗體滴度水平、IL-4、IL-10、IL-2、IFN-γ水平,流式細胞測定各組淋巴組織中濾泡輔助T細胞(follicular helperT cell,Tfh)的比例及應用RT-PCR測定各組淋巴組織中IL-21和扠狀頭轉錄因子FoxP3的錶達.結果 實驗組移植術後14 d乙肝抗體滴度明顯高于對照組(F=29.886,P=0.010),皮膚移植物的存活時間也明顯長于對照組(F=29.772,P=0.011),ELISA測定血清Th2類因子IL-4,IL-10的水平實驗組明顯低于對照組(F=42.102,P=0.009;F=23.734,P-0.015),Th1類因子IL-2和IFN-γ水平明顯高于對照組(F=18.156,P=0.0020;F =90.042,P =0.003).實驗組混閤淋巴細胞RT-PCR測定IL-21、FoxP3的錶達水平明顯高于對照組(F=9.784,P =0.048；F=27.883,P=0.012).結論 CsA聯閤IL-2融閤蛋白能夠增彊乙肝疫苗的抗體滴度,同時能夠延長小鼠皮膚移植物的存活時間,可能與促進IL-21及FoxP3基因的錶達有關.
목적 연구배포소A(CsA)연합IL-2융합단백대소서이식술후을간역묘적체액면역반응영향,심구간이식술후예방을형간염적유효방법.방법 장C57BL/6소서40지,분위실험조、대조조화공백조,미면역조,매조10지.실험조、대조조행피부이식후(DBA소서위공체),실험조급여CsA(30 mg/kg,복강내주사)+IL-2융합단백,대조조지급여CsA,공백조지행피부이식,3조균급여을간역묘안재시,미면역조불작임하처리.14 d후측정각조을간항체적도수평、IL-4、IL-10、IL-2、IFN-γ수평,류식세포측정각조림파조직중려포보조T세포(follicular helperT cell,Tfh)적비례급응용RT-PCR측정각조림파조직중IL-21화차상두전록인자FoxP3적표체.결과 실험조이식술후14 d을간항체적도명현고우대조조(F=29.886,P=0.010),피부이식물적존활시간야명현장우대조조(F=29.772,P=0.011),ELISA측정혈청Th2류인자IL-4,IL-10적수평실험조명현저우대조조(F=42.102,P=0.009;F=23.734,P-0.015),Th1류인자IL-2화IFN-γ수평명현고우대조조(F=18.156,P=0.0020;F =90.042,P =0.003).실험조혼합림파세포RT-PCR측정IL-21、FoxP3적표체수평명현고우대조조(F=9.784,P =0.048；F=27.883,P=0.012).결론 CsA연합IL-2융합단백능구증강을간역묘적항체적도,동시능구연장소서피부이식물적존활시간,가능여촉진IL-21급FoxP3기인적표체유관.
Objective To study the effect on humoral immunity with combination of CsA and IL-2 fusion protein. Methods Forty C57/B6 mice were evenly divided into four groups,after receiving skin graft from DBA mouse.Mice in the experimental group was given CsA(30 mg/kg,ip) plus IL-2/Fc (1μg,ip) while the control group was only given CsA,each group was given HBV vaccine after skin graft surgery (2 μg,im),while blank group was only given vaccine after skin transplantation.The fourth group were left intact.Fourteen days later,the level of HBSAb,IL-4,IL-10,IFN-γ,IL-2 were measured with ELISA and IL-21and FoxP3 expression level and Tfh percentage of mixed lymphocytes detected. Results The HBSAb level in experimental group is significantly higher than that in the control group and the survival time of skin graft is longer than that in the control group ( F =29.886,P =0.010 ; F =29.772,P =0.011).IL-2,IFN-γ are significantly higher than that in the control group( F =18.156,P =0.0020;F =90.042,P =0.003 ),but the Th2 cytokines such as IL-4,IL-10 are lower ( F =42.102,P =0.009 ; F =23.734,P =0.015 ).The expression level of both IL-21and FoxP3 are significantly higher than control group( F =9.784,P =0.048 ;F =27.883,P =0.012). Conclusions Combination of CsA and IL-2 fusion protein can significantly enhance the immunogenicity of HBV vaccine and prolong graft survival time.It may be related to the higher expression level of IL-21and FoxP3.
