中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2011年
9期
805-809
,共5页
孙谦%张嵘%喻华%李轶%胡云健%沈强%李国雄%曹俊敏%杨伟%王琴%周宏伟%胡燕燕
孫謙%張嶸%喻華%李軼%鬍雲健%瀋彊%李國雄%曹俊敏%楊偉%王琴%週宏偉%鬍燕燕
손겸%장영%유화%리질%호운건%침강%리국웅%조준민%양위%왕금%주굉위%호연연
嗜麦芽窄食单胞菌%喹诺酮类%抗药性,细菌%基因,细菌%突变
嗜麥芽窄食單胞菌%喹諾酮類%抗藥性,細菌%基因,細菌%突變
기맥아착식단포균%규낙동류%항약성,세균%기인,세균%돌변
Stenotrophomonas maltophilia%Quinolones%Drug resistance,bacterial%Genes,bacterial%Mutation
目的 描述新型突变喹诺酮耐药基因Smqnr及其在嗜麦芽窄食单胞菌的分布，初步探讨Smqnr基因与喹诺酮抗生素耐药之间的关系。方法 嗜麦芽窄食单胞菌的鉴定采用VITEK全自动细菌鉴定和药敏系统，采用标准琼脂稀释法测定替加环素、氯霉素、头孢他啶、复方磺胺甲(恶)唑、替卡西林/克拉维酸、左氧氟沙星、莫西沙星7种抗生素对442株嗜麦芽窄食单胞菌的最低抑菌浓度(MIC)。聚合酶链反应(PCR)扩增全长Smqnr基因，测序后采用DNAMAN软件比对序列之间的差异，并构建系谱树分析不同Smqnr基因之间亲缘性关系。结果 嗜麦芽窄食单胞菌对7种抗菌药物有不同程度的耐药，且在5％ ～50％之间变动，左氧氟沙星的抗菌活性较好，耐药率仅为6.11％ (27/442)，抗菌活性最好的药物为莫西沙星，耐药率为5.88％( 25/442)，442株嗜麦芽窄食单胞菌中114株检测到Smqnr基因，检出率为25.79％ (114/442)，包括11种已发现的Smqnr和20种新型突变的Smqnr基因（Smqnr 28 ～ 47），新型突变的Smqnr基因是由于部分碱基发生突变导致相应的219位翻译氨基酸位点发生改变。耐药株Smqnr基因检出率为42.30％ (11/26)，中介株Smqnr基因检出率为34.37％( 11/32),敏感株检出率23.95％ (92/384)，敏感株中MIC为0.125 μg/ml的嗜麦芽窄食单胞菌Smqnr基因检出率最高，为37.78％。结论Smqnr基因编码区高度多态，新型突变的Smqnr基因是由于部分碱基发生突变导致相应的219位翻译氨基酸位点发生改变所致。Smqnr基因在嗜麦芽窄食假单胞菌中的检出率较高，分布也存在较大差异。
目的 描述新型突變喹諾酮耐藥基因Smqnr及其在嗜麥芽窄食單胞菌的分佈，初步探討Smqnr基因與喹諾酮抗生素耐藥之間的關繫。方法 嗜麥芽窄食單胞菌的鑒定採用VITEK全自動細菌鑒定和藥敏繫統，採用標準瓊脂稀釋法測定替加環素、氯黴素、頭孢他啶、複方磺胺甲(噁)唑、替卡西林/剋拉維痠、左氧氟沙星、莫西沙星7種抗生素對442株嗜麥芽窄食單胞菌的最低抑菌濃度(MIC)。聚閤酶鏈反應(PCR)擴增全長Smqnr基因，測序後採用DNAMAN軟件比對序列之間的差異，併構建繫譜樹分析不同Smqnr基因之間親緣性關繫。結果 嗜麥芽窄食單胞菌對7種抗菌藥物有不同程度的耐藥，且在5％ ～50％之間變動，左氧氟沙星的抗菌活性較好，耐藥率僅為6.11％ (27/442)，抗菌活性最好的藥物為莫西沙星，耐藥率為5.88％( 25/442)，442株嗜麥芽窄食單胞菌中114株檢測到Smqnr基因，檢齣率為25.79％ (114/442)，包括11種已髮現的Smqnr和20種新型突變的Smqnr基因（Smqnr 28 ～ 47），新型突變的Smqnr基因是由于部分堿基髮生突變導緻相應的219位翻譯氨基痠位點髮生改變。耐藥株Smqnr基因檢齣率為42.30％ (11/26)，中介株Smqnr基因檢齣率為34.37％( 11/32),敏感株檢齣率23.95％ (92/384)，敏感株中MIC為0.125 μg/ml的嗜麥芽窄食單胞菌Smqnr基因檢齣率最高，為37.78％。結論Smqnr基因編碼區高度多態，新型突變的Smqnr基因是由于部分堿基髮生突變導緻相應的219位翻譯氨基痠位點髮生改變所緻。Smqnr基因在嗜麥芽窄食假單胞菌中的檢齣率較高，分佈也存在較大差異。
목적 묘술신형돌변규낙동내약기인Smqnr급기재기맥아착식단포균적분포，초보탐토Smqnr기인여규낙동항생소내약지간적관계。방법 기맥아착식단포균적감정채용VITEK전자동세균감정화약민계통，채용표준경지희석법측정체가배소、록매소、두포타정、복방광알갑(악)서、체잡서림/극랍유산、좌양불사성、막서사성7충항생소대442주기맥아착식단포균적최저억균농도(MIC)。취합매련반응(PCR)확증전장Smqnr기인，측서후채용DNAMAN연건비대서렬지간적차이，병구건계보수분석불동Smqnr기인지간친연성관계。결과 기맥아착식단포균대7충항균약물유불동정도적내약，차재5％ ～50％지간변동，좌양불사성적항균활성교호，내약솔부위6.11％ (27/442)，항균활성최호적약물위막서사성，내약솔위5.88％( 25/442)，442주기맥아착식단포균중114주검측도Smqnr기인，검출솔위25.79％ (114/442)，포괄11충이발현적Smqnr화20충신형돌변적Smqnr기인（Smqnr 28 ～ 47），신형돌변적Smqnr기인시유우부분감기발생돌변도치상응적219위번역안기산위점발생개변。내약주Smqnr기인검출솔위42.30％ (11/26)，중개주Smqnr기인검출솔위34.37％( 11/32),민감주검출솔23.95％ (92/384)，민감주중MIC위0.125 μg/ml적기맥아착식단포균Smqnr기인검출솔최고，위37.78％。결론Smqnr기인편마구고도다태，신형돌변적Smqnr기인시유우부분감기발생돌변도치상응적219위번역안기산위점발생개변소치。Smqnr기인재기맥아착식가단포균중적검출솔교고，분포야존재교대차이。
Objective To describe the novel variants of the Smqnr family of quinolone resistance genes and their distribution in clinical isolates of Stenotrophomonas maltophilia, and investigate the relationship between Smqnr and quinolone resistance. MethodsThe identification of 442 strains of Stenotrophomonas maltophilia were performed by VITEK automated identification and susceptibility. Minimum inhibitory concentrations of tigecycline, chloramphenicol, ceftazidime, compound sulfamethoxazole,ticarcillin/clavulanic acid, levofloxacin and moxifloxacin against Stenotrophomonas maltophilia were detected by standard agar dilution method. Full length of Smqnr gene was amplified by polymerase chain reaction (PCR) and sequenced. DNAMAN software was used to compare the sequence divergence and construct the genealogical tree to analyze the phylogenetic relationships of Smqnr family. Results Stenotrophomonas maltophilia was resistant to the 7 kinds of clinical antibiotics in various extent ( from 5％ to 50％ ). Levofloxacin showed s good antibacterial activity with the resistance rate of 6. 11％ (27/442), nevertheless the best was moxifloxacin with the resistance rate of 5. 88％ (25/442). Smqnr gene was detected in 114 of 442 strains of Stenotrophomonas maltophilia[25.79％ (114/442)], including 11 known genes and 20 novel variants of the Smqnr genes ( Smqnr28-47 ) which was caused by several genes mutation changing the translation of 219 amino acids. The gene detection rate of resistant, intermediate and sensitive strains was 42. 30％ (11/26), 34. 37％ (11/32) and 23.95％ (92/384), respectively. The Smqnr gene harbored the highest detection rote (37. 78％ ) in the sensitive strains of Stenotrophomonas maltophilia with minimal inhibitory concentration of 0. 125 μg/ml. Conclusions The gene coding region of Smqnr is highly polymorphic and the novel variants of Smqnr gene are caused by several genes mutation changing the translation of 219 amino acids. Smqnr gene in Stenotrophomonas maltophilia has a high detection rate and different distribution.
