CAJ | 학술논문

目的:研究Z,E-butylidedephthalide(Bdph)对激光诱导的大鼠脉络膜新生血管(CNV)和兔眼脉络膜血流的影响.方法:雄性Brown Norway大鼠行Nd:YAG激光诱导眼底Bruch膜破裂,而后分别予30 mg/kg和15 mg/kg Bdph 1 次/d腹腔注射,连续4 wk.2 wk及4 wk末行眼底荧光血管造影检查.4 wk末制作脉络膜平片测量新生血管的面积.用1 g/L Z,E-butylidenephthalide给雌性新西兰大白兔点眼,采用彩色微球技术测量大白兔眼部血流的变化.结果:和对照组相比,Bdph 30 mg/kg和15 mg/kg组大鼠眼底血管荧光渗漏明显减少,P＜0.01;在此两组中,眼底荧光血管造影测定的新生血管面积以及脉络膜平片测定的新生血管面积都比对照组减少.10 g/L Z,E-butylideneph-thalide滴入兔眼30和60 min后脉络膜血流均比对照组增加,P＜0.05.结论:Z,E-butylidedephthalide可以抑制大鼠脉络膜新生血管的生长,增强兔眼脉络膜血流,可能成为治疗年龄相关性黄斑变性的新药.
목적:연구Z,E-butylidedephthalide(Bdph)대격광유도적대서맥락막신생혈관(CNV)화토안맥락막혈류적영향.방법:웅성Brown Norway대서행Nd:YAG격광유도안저Bruch막파렬,이후분별여30 mg/kg화15 mg/kg Bdph 1 차/d복강주사,련속4 wk.2 wk급4 wk말행안저형광혈관조영검사.4 wk말제작맥락막평편측량신생혈관적면적.용1 g/L Z,E-butylidenephthalide급자성신서란대백토점안,채용채색미구기술측량대백토안부혈류적변화.결과:화대조조상비,Bdph 30 mg/kg화15 mg/kg조대서안저혈관형광삼루명현감소,P＜0.01;재차량조중,안저형광혈관조영측정적신생혈관면적이급맥락막평편측정적신생혈관면적도비대조조감소.10 g/L Z,E-butylideneph-thalide적입토안30화60 min후맥락막혈류균비대조조증가,P＜0.05.결론:Z,E-butylidedephthalide가이억제대서맥락막신생혈관적생장,증강토안맥락막혈류,가능성위치료년령상관성황반변성적신약.
AIM: To investigate the effect of Z,E-butylidedephthalide (Bdph) on laser-induced experimental choroidal neovascularization (CNV) in rat model and choroid blood flow in rabbits'eyes.METHODS: Male Brown Norway rats were treated with Nd:YAG laser to break Bruch's membrane. Thirty mg/kg and 15 mg/kg Bdph were given daily through intraperitoneal injection for 4 weeks after laser treatment. Fluorescein angiography (FA) and choroidal flat mount were used to measure the development of CNV. Female New Zealand white rabbits' eyes were instilled with 10g/L Z,E-butylidenephthalide solution,and ocular blood flow was measured with colored microsphere technique. RESULTS: The intensity of fluorescein leakage, indicating the ocular lesion, decreased significantly in group Bdph 30mg/kg and 15mg/kg, as compared to the control at P＜0.01.The area of neovascularization checked by FA in both groups of Bdph, at 30mg/kg and 15mg/kg decreased significantly compared to the control group at P＜0.05. On the choroid flat mount, the areas of CNV were also smaller in both Bdph groups than in control group. One percent Z,E-butylidenephthalide solution instilled into rabbits' eyes could improve the choroid blood flow at 30 and 60 minutes after drug instillation (P＜0.05).CONCLUSION: Z,E-butylidedephthalide could inhibit the development of CNV in the rat eyes and increase the choroid blood flow in the rabbit eyes. These results suggest that Z,E-butylidedephthalide may be a good agent for the treatment of age-related macular degeneration(ARMD).