农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2008年
2期
33-35,41
,共4页
李刚%刘菊华%谭光兰%徐碧玉%金志强
李剛%劉菊華%譚光蘭%徐碧玉%金誌彊
리강%류국화%담광란%서벽옥%금지강
Lilium longiflorum%Agrobacterium-modiatod transformation%T-DNA%Insertion mutation
[Objective] The aim of this study is to obtain transgenic Lilium longiflorum Thumb. [Method] A two-step method of explant and the T-DNA integration technique were employed to transform Lilium longiflorum via Agrobacterium mediated method. [Result] The best infection effect appeared under the OD600 value of Agrobacterium within 0.6 -0.8, the addition of 250 mg/L AS could increase the transformation efficiency. The optimal concentration of G418 for screening is 50 mg/L. Some putative transgenic plants of Lilium longiflorum with resistance to G418 showed positive in PCR, preliminarily pro-ring that T-DNA gene had integrated into the genome of lily. [Conclusion] The study may lay a foundation for breeding excellent lily varieties through T-DNA integration technique.
