CAJ | 학술논문

为探讨醇溶蛋白在小麦杂种后代的遗传特点,选用2个小麦杂交组合(苏引10号×晋农190、晋麦47×晋农190),用酸性聚丙烯酰胺凝胶电泳(APAGE,pH 3.1)对其亲本及后代材料(F_1、F_2代)进行了电泳分析.结果表明,醇溶蛋白在F_1代表现出共显性和倾母性(剂量效应)遗传特点;F_2代蛋白谱带发生分离,具体表现为:Gli-13.9、23.2、26.0、34.8、40.2、46.2、50.0、71.5、73.8等9个组分的分离符合一对基因的分离规律,而Gli-12.7、15.7、16.5、19.1、26.9、30.5、30.8、32.2、76.8等9个组分的分离符合两对基因的分离规律;Gli-17.8既不符合一对基因的分离规律,又不符合两对基因的分离规律.这表明前9种蛋白受控于一对基因,后9种蛋白受控于两对基因;最后一种蛋白尚无法确定,需借助双向电泳做进一步研究.
위탐토순용단백재소맥잡충후대적유전특점,선용2개소맥잡교조합(소인10호×진농190、진맥47×진농190),용산성취병희선알응효전영(APAGE,pH 3.1)대기친본급후대재료(F_1、F_2대)진행료전영분석.결과표명,순용단백재F_1대표현출공현성화경모성(제량효응)유전특점;F_2대단백보대발생분리,구체표현위:Gli-13.9、23.2、26.0、34.8、40.2、46.2、50.0、71.5、73.8등9개조분적분리부합일대기인적분리규률,이Gli-12.7、15.7、16.5、19.1、26.9、30.5、30.8、32.2、76.8등9개조분적분리부합량대기인적분리규률;Gli-17.8기불부합일대기인적분리규률,우불부합량대기인적분리규률.저표명전9충단백수공우일대기인,후9충단백수공우량대기인;최후일충단백상무법학정,수차조쌍향전영주진일보연구.
In order to explore genetic characteristics of wheat gliadins components, the parents and F_1, F_2 generations of two crosses("Suyin 10 ×Jinnong 190", "Jinmai 47 × Jinnong 190") were studied by APAGE. It was showed that wheat gliadins were codominant and the female parent prevailed in F_1 ; there were 9 bands segarated as a pair of gene, for example, Gli-23.2, 34.8, 40.2, 46.2, 71.5, 73.8, 13.9, 26.0, 50.0 and there were 9 bands separated as two pairs of genes in F_2, in addition, Gli-17.8 did not follow with any one of them. It indicated that there were 9 gliadins controled by single gene, and an other 9 gliadins controled by two pairs of genes, but Gli-17.8 could not be determinated.