CAJ | 학술논문

从饲料用麦麸中分离出的12种细菌均能支持无菌家蝇Musca domestica L.幼虫在胰化酪蛋白大豆卵黄琼脂(Trypticase Soy Egg Yolk Agar,TSEYA)培养基中完成整个生长发育过程.1)幼虫在接种香味类香味菌Myroidesodoratimimus的TSEYA培养基中生长时间最短,仅需97. 61±1.14 h;2)幼虫在接种醋酸钙不动杆菌Acinetobactercalcoaceticus的TSEYA培养基中的化蛹率可达到86. 81%;3)从接种嗜水汽单胞菌Aeromonas hydrophila的TSEYA培养基中得到的蝇蛹重量最高,达到20.15±0.23 mg/个;4)除铜绿假单胞菌Pseudomonas aeruginosa饲养的家蝇羽化率较低(60.87%)外,其余各种细菌饲养的羽化率在84. 33%~ 97. 47%之间.此外,枯草芽孢杆菌Bacillussubtilis、香味类香味菌、聚团肠杆菌Enterobacter agglomerans以及成团肠杆菌Pantoea agglomerans可作为单一营养来源支持幼虫完成整个生长发育过程.对枯草芽孢杆菌、金黄色葡萄球菌Staphylococcus aurous、成团肠杆菌、大肠杆菌Escherichia coli、奇异变形杆菌Proteus mirabilis以及香味类香味菌中各种营养成分进行分析,结果发现,6种细菌均能提供大量的维生素B3(0. 105~1.08g/kg).在氨基酸方面,香味类香味菌和枯草芽孢杆菌中的10种昆虫必需氨基酸含量与总氨基酸含量之比(Essential amino acid/Total amino acid,EAA/TAA)最高,而金黄色葡萄球菌最低.这个比例与蛹重呈正相关(P= 0.031).在脂肪酸相对含量方面,金黄色葡萄球菌具有最高的饱和脂肪酸含量(76. 38%),香味类香味菌含有56. 79%的不饱和脂肪酸,而枯草芽孢杆菌则具有最高的支链脂肪酸含量(42.16%).
종사료용맥부중분리출적12충세균균능지지무균가승Musca domestica L.유충재이화락단백대두란황경지(Trypticase Soy Egg Yolk Agar,TSEYA)배양기중완성정개생장발육과정.1)유충재접충향미류향미균Myroidesodoratimimus적TSEYA배양기중생장시간최단,부수97. 61±1.14 h;2)유충재접충작산개불동간균Acinetobactercalcoaceticus적TSEYA배양기중적화용솔가체도86. 81%;3)종접충기수기단포균Aeromonas hydrophila적TSEYA배양기중득도적승용중량최고,체도20.15±0.23 mg/개;4)제동록가단포균Pseudomonas aeruginosa사양적가승우화솔교저(60.87%)외,기여각충세균사양적우화솔재84. 33%~ 97. 47%지간.차외,고초아포간균Bacillussubtilis、향미류향미균、취단장간균Enterobacter agglomerans이급성단장간균Pantoea agglomerans가작위단일영양래원지지유충완성정개생장발육과정.대고초아포간균、금황색포도구균Staphylococcus aurous、성단장간균、대장간균Escherichia coli、기이변형간균Proteus mirabilis이급향미류향미균중각충영양성분진행분석,결과발현,6충세균균능제공대량적유생소B3(0. 105~1.08g/kg).재안기산방면,향미류향미균화고초아포간균중적10충곤충필수안기산함량여총안기산함량지비(Essential amino acid/Total amino acid,EAA/TAA)최고,이금황색포도구균최저.저개비례여용중정정상관(P= 0.031).재지방산상대함량방면,금황색포도구균구유최고적포화지방산함량(76. 38%),향미류향미균함유56. 79%적불포화지방산,이고초아포간균칙구유최고적지련지방산함량(42.16%).
A total of 12 bacteria species isolated from wheat bran are able to support. the development of housefly larvae into pupation when inoculated on trypticase soy egg yolk agar (TSEYA) medium alone.The larvae which have the fastest growth rate (97.61 ± 1. 14 h) were grown on the trypticase soy egg yolk agar medium inoculated with Myroides odoratimimusi. Aeromonas hydrophila helped the development of larvae into pupae at a rate of 86. 81% , and Acinetobacter calcoaceticus supported the growth of pupae with the maximum weight (20. 15 ± 0. 23 mg). With the exception of Pseudomonas aeruginosa which rendered a very low rate of housefly emergence (60. 87 %) , all other bacteria could achieve high rates of eclosion ranging from 84. 33 to 97. 47%. Moreover, the housefly larvae could use four bacteria, including Enterobacter agglomerans, Pantoea agglomerans, M. odoratimimus and Bacillus subtilis, as sole nutriments to complete the whole development process. Six bacteria, B. subtilis, Staphylococcus aurous,Proteus mirabilis, P. agglomerans, M. odoratimimus and Escherichia coli, were selected to analyze their amino acid composition, content of fatty acids and vitamin B3. All selected bacteria had plenty of Vitamin B3 (0. 105 to 1. 08 g/kg). M. odoratimimus and B, subtilis had the highest proportion of essential amino acids and total amino acids, and the lowest one was S. aureus. This proportion was correlated with pupae weight (p =0. 031), The bacterium, which had the highest relative content of saturated fatty acids,unsaturated fatty acids and branch- saturated fatty acids were S. aureus (76. 38 %) , M. odoratimimus (56. 79 %) and B. Subtilis (42. 16 %) , respectively.