CAJ | 학술논문

目的应用流式细胞术(FCM)检测双表型急性白血病(BAL)的免疫表型.方法采用四色FCM检测23例BAL患者的免疫表型.结果 23例BAL患者中有10例(43.4%)表达cCD3,16例(69.6%)表达cCD79a,20例(87.0%)表达cMPO,14例(60.9%)表达TdT,19例(82.6%)表达CD34,20例(87.0%)表达CD117,同时表达髓系和B系抗原者13例(56.5%),均共同表达cCD79a和cMPO;同时表达髓系和T系抗原者7例(30.4%),均共同表达cCD3和cMPO;同时表达T系和B系抗原者3例(13.04%),均共同表达cCD3和cCD79a.结论 cCD3、cCD79a、cMPO为系列特异性抗原标志,对诊断及鉴别BAL具有重要意义,FCM是目前诊断BAL特异口靠的方法,在临床白血病治疗和预后方面有重要的指导意义.
목적 응용류식세포술(FCM)검측쌍표형급성백혈병(BAL)적면역표형.방법 채용사색FCM검측23례BAL환자적면역표형.결과 23례BAL환자중유10례(43.4%)표체cCD3,16례(69.6%)표체cCD79a,20례(87.0%)표체cMPO,14례(60.9%)표체TdT,19례(82.6%)표체CD34,20례(87.0%)표체CD117,동시표체수계화B계항원자13례(56.5%),균공동표체cCD79a화cMPO;동시표체수계화T계항원자7례(30.4%),균공동표체cCD3화cMPO;동시표체T계화B계항원자3례(13.04%),균공동표체cCD3화cCD79a.결론 cCD3、cCD79a、cMPO위계렬특이성항원표지,대진단급감별BAL구유중요의의,FCM시목전진단BAL특이구고적방법,재림상백혈병치료화예후방면유중요적지도의의.
Objective To detect double immuno-phenotype of acute leukemia by the application of flow cytometry (FCM). Methods Four-color FCM was used to analyse 23 cases of acute leukemia of double immune phenotype. Results Among 23 cases, cCD3 was expressed in 10 cases (43.4 %), cCD79a in 16 cases (69.6 %), cMPO in 20 cases (87.0 %), TdT in 14 cases (60.9 %), CD34 in 19 cases (82.6 %) and CD117 in 20 cases (87.0 %). 13 cases (56.5 %) expressed both myeloid and B lymphocyte antigens, in which cCD79a and cMPO were positively expressed, 7 cases (30.4 %) were found to express both myeloid and T lymphocyte antigens of cCD3 and cMPO 3 cases (13.04 %) were positive for both cCD3 and cCD79a. Conclusion cCD3,cCD79a, and cMPO were lineage specific antigen markers, which could be used for diagnosis and differential double phenotype leukemias. FCM is a useful and reliable method for diagnosis of acute leukemia, and guidance of treatment and prognosis of leukemia in clinical.