中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2008年
8期
1267-1268
,共2页
李士坤%徐俊%陈克河%任庆梅
李士坤%徐俊%陳剋河%任慶梅
리사곤%서준%진극하%임경매
DNA聚合酶β%Eca-109细胞%X-射线修复交联-补充蛋白1
DNA聚閤酶β%Eca-109細胞%X-射線脩複交聯-補充蛋白1
DNA취합매β%Eca-109세포%X-사선수복교련-보충단백1
DNA polymerase beta%Eca-109 cell line%X-ray repair cross-complementing protein 1
目的 探讨恶性细胞在逆转化过程中DNA聚合酶β及X-射线修复交联-补充蛋白1(XRCC1)表达的变化.方法 将Eca-109细胞分为诱导组(加8-溴-cAMP)和对照组.两组同等条件下培养48 h,用免疫细胞化学的方法 检测DNA聚合酶β及XRCCl的表达.结果 与对照组相比,诱导组DNA聚合酶β与XRCC1的表达均减弱(P＜0.05).结论 分化诱导剂能下调DNA聚合酶β及XRCC1的表达,高表达的DNA聚合酶β及XRCC1可能是细胞恶性化的表现.
目的 探討噁性細胞在逆轉化過程中DNA聚閤酶β及X-射線脩複交聯-補充蛋白1(XRCC1)錶達的變化.方法 將Eca-109細胞分為誘導組(加8-溴-cAMP)和對照組.兩組同等條件下培養48 h,用免疫細胞化學的方法 檢測DNA聚閤酶β及XRCCl的錶達.結果 與對照組相比,誘導組DNA聚閤酶β與XRCC1的錶達均減弱(P＜0.05).結論 分化誘導劑能下調DNA聚閤酶β及XRCC1的錶達,高錶達的DNA聚閤酶β及XRCC1可能是細胞噁性化的錶現.
목적 탐토악성세포재역전화과정중DNA취합매β급X-사선수복교련-보충단백1(XRCC1)표체적변화.방법 장Eca-109세포분위유도조(가8-추-cAMP)화대조조.량조동등조건하배양48 h,용면역세포화학적방법 검측DNA취합매β급XRCCl적표체.결과 여대조조상비,유도조DNA취합매β여XRCC1적표체균감약(P＜0.05).결론 분화유도제능하조DNA취합매β급XRCC1적표체,고표체적DNA취합매β급XRCC1가능시세포악성화적표현.
Objective To study the change of DNA polymerase beta and XRCC1's expression during malignant celI differentiation.Methods The Eca-109 cells were divided inm 2 groups:differentiation group which cultured with 8-Br-cAMP and control group.The 2 groups cells were cultured 48h simultaneously.The immunocytochemistry was performed to detect the expression of DNA polymerase beta and XRCC1.Results Compared with control group,the expression of DNA polymerase beta and XRCC1 was decreased simultaneously(P＜0.05).Conclusion The differentiation agent can down-regulate the expression of DNA polymerase beta and XRCC1,suggesting that overexpressed DNA polymerase beta and XRCC1 maybe result in mutator phenotype.
