中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2011年
7期
625-629
,共5页
基质细胞衍生因子-1%氧诱导视网膜病变%疾病模型/小鼠%视网膜新生血管
基質細胞衍生因子-1%氧誘導視網膜病變%疾病模型/小鼠%視網膜新生血管
기질세포연생인자-1%양유도시망막병변%질병모형/소서%시망막신생혈관
Stromal cell-derived factor-1%Oxygen-induced retinopathy%Disease models/mouse%Retinal neovascularization
背景 早产儿视网膜病变(ROP)的进展与多种新生血管调控因子有关,而基质细胞衍生因子-1(SDF-1)在氧诱导视网膜病变(OIR)动物模型视网膜中的表达及其作用机制尚不明确.目的 对SDF-1在OIR小鼠模型视网膜中的表达进行定位和定量分析.方法 应用随机数字表法将动物随机分组.将20只7日龄C57BL/6J幼鼠暴露在体积分数(75±2)%浓度的高氧状态下5d,随后在正常氧环境下5d,作为OIR组;另20只同日龄幼鼠作为正常对照组.通过免疫组织化学染色和实时荧光定量聚合酶链反应(real-time PCR)法观察视网膜的SDF-1的蛋白表达以及SDF-1mRNA的变化.结果 OIR组12日龄小鼠的视网膜神经节细胞层可见SDF-1蛋白呈阳性表达;OIR组17日龄小鼠的神经节细胞层、内层视网膜的血管内皮细胞、新生血管内皮细胞可见SDF-1蛋白呈强阳性表达;正常对照组12日龄小鼠SDF-1蛋白和正常对照组17日龄小鼠SDF-1蛋白微弱表达于内层视网膜及视网膜血管附近.OIR组17日龄小鼠的SDF-1mRNA表达水平明显高于OIR组12日龄小鼠(P＜0.01)及正常对照组17日龄小鼠(P＜0.01);OIR组12日龄小鼠SDF-1mRNA表达水平明显低于正常对照组12日龄小鼠(P＜0.05).OIR组17日龄小鼠的SDF-1mRNA表达水平明显高于OIR组12日龄小鼠(t=8.072,P＜0.05)和正常对照组17日龄小鼠(t=10.026,P＜0.05);OIR组12日龄小鼠SDF-1mRNA表达水平明显低于正常对照组12日龄小鼠(t=4.336,P＜0.05).结论 SDF-1在相对低氧状态下的视网膜中表达明显上调,因而可促进OIR的视网膜新生血管形成.
揹景 早產兒視網膜病變(ROP)的進展與多種新生血管調控因子有關,而基質細胞衍生因子-1(SDF-1)在氧誘導視網膜病變(OIR)動物模型視網膜中的錶達及其作用機製尚不明確.目的 對SDF-1在OIR小鼠模型視網膜中的錶達進行定位和定量分析.方法 應用隨機數字錶法將動物隨機分組.將20隻7日齡C57BL/6J幼鼠暴露在體積分數(75±2)%濃度的高氧狀態下5d,隨後在正常氧環境下5d,作為OIR組;另20隻同日齡幼鼠作為正常對照組.通過免疫組織化學染色和實時熒光定量聚閤酶鏈反應(real-time PCR)法觀察視網膜的SDF-1的蛋白錶達以及SDF-1mRNA的變化.結果 OIR組12日齡小鼠的視網膜神經節細胞層可見SDF-1蛋白呈暘性錶達;OIR組17日齡小鼠的神經節細胞層、內層視網膜的血管內皮細胞、新生血管內皮細胞可見SDF-1蛋白呈彊暘性錶達;正常對照組12日齡小鼠SDF-1蛋白和正常對照組17日齡小鼠SDF-1蛋白微弱錶達于內層視網膜及視網膜血管附近.OIR組17日齡小鼠的SDF-1mRNA錶達水平明顯高于OIR組12日齡小鼠(P＜0.01)及正常對照組17日齡小鼠(P＜0.01);OIR組12日齡小鼠SDF-1mRNA錶達水平明顯低于正常對照組12日齡小鼠(P＜0.05).OIR組17日齡小鼠的SDF-1mRNA錶達水平明顯高于OIR組12日齡小鼠(t=8.072,P＜0.05)和正常對照組17日齡小鼠(t=10.026,P＜0.05);OIR組12日齡小鼠SDF-1mRNA錶達水平明顯低于正常對照組12日齡小鼠(t=4.336,P＜0.05).結論 SDF-1在相對低氧狀態下的視網膜中錶達明顯上調,因而可促進OIR的視網膜新生血管形成.
배경 조산인시망막병변(ROP)적진전여다충신생혈관조공인자유관,이기질세포연생인자-1(SDF-1)재양유도시망막병변(OIR)동물모형시망막중적표체급기작용궤제상불명학.목적 대SDF-1재OIR소서모형시망막중적표체진행정위화정량분석.방법 응용수궤수자표법장동물수궤분조.장20지7일령C57BL/6J유서폭로재체적분수(75±2)%농도적고양상태하5d,수후재정상양배경하5d,작위OIR조;령20지동일령유서작위정상대조조.통과면역조직화학염색화실시형광정량취합매련반응(real-time PCR)법관찰시망막적SDF-1적단백표체이급SDF-1mRNA적변화.결과 OIR조12일령소서적시망막신경절세포층가견SDF-1단백정양성표체;OIR조17일령소서적신경절세포층、내층시망막적혈관내피세포、신생혈관내피세포가견SDF-1단백정강양성표체;정상대조조12일령소서SDF-1단백화정상대조조17일령소서SDF-1단백미약표체우내층시망막급시망막혈관부근.OIR조17일령소서적SDF-1mRNA표체수평명현고우OIR조12일령소서(P＜0.01)급정상대조조17일령소서(P＜0.01);OIR조12일령소서SDF-1mRNA표체수평명현저우정상대조조12일령소서(P＜0.05).OIR조17일령소서적SDF-1mRNA표체수평명현고우OIR조12일령소서(t=8.072,P＜0.05)화정상대조조17일령소서(t=10.026,P＜0.05);OIR조12일령소서SDF-1mRNA표체수평명현저우정상대조조12일령소서(t=4.336,P＜0.05).결론 SDF-1재상대저양상태하적시망막중표체명현상조,인이가촉진OIR적시망막신생혈관형성.
Background The development of retinopathy of prematurity(ROP) is associated with many regulatory cytokines related to neovascularization;however,the retinal expression and regulated mechanism of stromal cell-derived factor-1 (SDF-1) in mouse model of oxygen-induced retinopathy (OIR) remain uncertain.Objective This study was to investigate the expression of SDF-1 in retina of mouse model of OIR.Methods Forty 7-day-old C57BL/6J mice were divided into OIR group and control group.In OIR group,20 mice were exposed to 75% oxygen for 5 days and then to room air for 5 days.In control group,20 mice were raised in room air.The expression of SDF-1 in retina of mice was studied by immunochemistry and quantified by real time reverse transcriptase polymerase chain reaction (RT-PCR).Results The positive immunohistochemical staining for SDF-1 was found mainly locating at the ganglion cell layer in 12-day-old mice of OIR group;the stronger positive immunohistochemical staining for SDF-1 was noted mainly locating at the ganglion cell layer,vascular endothelial cells of inner retina,neovascular endothelial cells in 17-day-old mice of OIR group;the delicate positive immunohistochemical staining for SDF-1 was both found mainly locating at the inner retina and being around the retinal vascular in 12-day-old mice of control group and 17-day-old mice of control group.The expression of SDF-1 mRNA in 17-day-old mice of OIR group was higher than that of 12-day-old mice of OIR group (t=8.072,P＜0.05)and 17-day-old mice of control group(t=10.026,P＜0.05),respectively.The expression of SDF-1 mRNA in 12-day-old mice of OIR group was lower than that of 12-day-old mice of control group (t=4.336,P＜0.05).Conclusion SDF-1 might improve the onset of retinal neovascularization of OIR.
