CAJ | 학술논문

目的探讨整体和离体照射所致的大鼠淋巴细胞T细胞受体(TCR)基因突变的一致性.方法 48只雌性大鼠随机分为6组,每组8只,分别取外周静脉血标本,经0、0.5、0.75、1.0、2.0和3.0 Gy照射.用相同方法照射各组大鼠,照后取外周血标本.所有淋巴细胞体外培养7 d后,用单克隆抗体直接免疫荧光标记流式细胞术检测TCR突变频率(TCRMF).结果整体和离体照射组TCR基因突变频率均呈剂量依赖性增高,两组间TCRMF比较,差异无统计学意义(t=-1.1～0.3,P＞0.05).结论整体和离体照射所致的大鼠淋巴细胞TCR基因突变频率一致,即外周血淋巴细胞经X射线照射体外培养后,流式细胞术检测,可客观准确的反映全身照射的情况.
목적 탐토정체화리체조사소치적대서림파세포T세포수체(TCR)기인돌변적일치성.방법 48지자성대서수궤분위6조,매조8지,분별취외주정맥혈표본,경0、0.5、0.75、1.0、2.0화3.0 Gy조사.용상동방법 조사각조대서,조후취외주혈표본.소유림파세포체외배양7 d후,용단극륭항체직접면역형광표기류식세포술검측TCR돌변빈솔(TCRMF).결과 정체화리체조사조TCR기인돌변빈솔균정제량의뢰성증고,량조간TCRMF비교,차이무통계학의의(t=-1.1～0.3,P＞0.05).결론 정체화리체조사소치적대서림파세포TCR기인돌변빈솔일치,즉외주혈림파세포경X사선조사체외배양후,류식세포술검측,가객관준학적반영전신조사적정황.
Objective To observe the consistency of T-cell receptor (TCR) genes mutation in lymphocytes in rats after irradiation in vivo and in vitro.Methods A total of 48 female rats were randomly divided into 6 equal groups.Peripheral blood samples from them were collected to separate the lymphocytes and then irradiated to X-ray irradiation with the dose rate of 200 cGy/min at the doses of 0,0.5,0.75,1.0,2.0,and 3.0 Gy,respectively.Then all the lymphocyte samples were cultured for 7 days.Flow cytometry with direct immunofluorescence was used to detect the TCR gene mutation.The levels of TCR gene mutant frequency (TCRMF) of different groups were calculated.Results The TCRMF levels of different groups after irradiation in vivo and in vitro all displayed a dose-dependent manner and there were no significant differences in the TCRMF between different dose irradiation groups(t = -1.1-0.3 ,P ＞0.05).Conclusions A consistency of TCRMF after irradiation in vivo and in vitro is proven.The results of TCRMF of peripheral blood lymphocytes irradiated in vitro by flow cytometry can precisely reflect the TCR genes mutation after whole-body irradiation.