中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2009年
8期
495-498
,共4页
王晓娟%车传珍%周昱男%刘仲萍%黄士昂
王曉娟%車傳珍%週昱男%劉仲萍%黃士昂
왕효연%차전진%주욱남%류중평%황사앙
T淋巴细胞,调节性%基因表达调控%移植物抗宿主病
T淋巴細胞,調節性%基因錶達調控%移植物抗宿主病
T림파세포,조절성%기인표체조공%이식물항숙주병
T-lymphocytes,regulatory%Gene expression regulation%Graft vs host disease
目的 研究患者异基因造血干细胞移植(allo-HSCT)前后骨髓或外周血单个核细胞中CD4+CD25+Foxp3+调节性T淋巴细胞(以下简称CD4+CD25+Foxp3+T细胞)的比率、Foxp3 mRNA和Foxp3蛋白的表达与急性移植物抗宿主病(aGVHD)的关系.方法 选择37例行HIA相合异基因造血干细胞移植(allo-HSCT))的患者作为研究对象.采用流式细胞术检测患者allo-HSCT前7天(-7 d)、移植当天(0 d)、移植后30天(+30d)、+60 d和+90 d时骨髓或外周血单个核细胞中CD4+CD25+Foxp3+T细胞所占的比率;定量聚合酶链反应(PCR)检测Foxp3 mRNA相对表达量;蛋白印迹(Western blot)法检测Foxp3的蛋白表达水平.结果 37例患者存移植后100 d内发生aGVHD 13例(aGVHD组),未发生aGVHD 24例(无aGVHD组).CD4+CD25+Foxp3+T细胞的比率在0 d时最低,与-7、+30、+60、+90d时比较.差异均有统计学意义(P<0.01);与aGVHD组比较,无aGVHD组患者的CD4+CD25+Foxp3+T细胞的比率明显升高,差异有统计学意义(P<0.01).aGVHD组患者移植后Foxp3的表达水平逐渐升高,但明显低于无aGVHD组,尤其在+60和+90 d时的差异有统计学意义(P<0.01).Western blot法检测结果 表明,aGVHD组受者的Foxp3蛋白表达也明显低于无aGVHD组.结论 CD4+CD25+Foxp3+T细胞对防止发生aGVHD具有重要作用,监测患者骨髓或外周血中CD4+CD25+Foxp3+T细胞的比率可以预测aGVHD的发生.
目的 研究患者異基因造血榦細胞移植(allo-HSCT)前後骨髓或外週血單箇覈細胞中CD4+CD25+Foxp3+調節性T淋巴細胞(以下簡稱CD4+CD25+Foxp3+T細胞)的比率、Foxp3 mRNA和Foxp3蛋白的錶達與急性移植物抗宿主病(aGVHD)的關繫.方法 選擇37例行HIA相閤異基因造血榦細胞移植(allo-HSCT))的患者作為研究對象.採用流式細胞術檢測患者allo-HSCT前7天(-7 d)、移植噹天(0 d)、移植後30天(+30d)、+60 d和+90 d時骨髓或外週血單箇覈細胞中CD4+CD25+Foxp3+T細胞所佔的比率;定量聚閤酶鏈反應(PCR)檢測Foxp3 mRNA相對錶達量;蛋白印跡(Western blot)法檢測Foxp3的蛋白錶達水平.結果 37例患者存移植後100 d內髮生aGVHD 13例(aGVHD組),未髮生aGVHD 24例(無aGVHD組).CD4+CD25+Foxp3+T細胞的比率在0 d時最低,與-7、+30、+60、+90d時比較.差異均有統計學意義(P<0.01);與aGVHD組比較,無aGVHD組患者的CD4+CD25+Foxp3+T細胞的比率明顯升高,差異有統計學意義(P<0.01).aGVHD組患者移植後Foxp3的錶達水平逐漸升高,但明顯低于無aGVHD組,尤其在+60和+90 d時的差異有統計學意義(P<0.01).Western blot法檢測結果 錶明,aGVHD組受者的Foxp3蛋白錶達也明顯低于無aGVHD組.結論 CD4+CD25+Foxp3+T細胞對防止髮生aGVHD具有重要作用,鑑測患者骨髓或外週血中CD4+CD25+Foxp3+T細胞的比率可以預測aGVHD的髮生.
목적 연구환자이기인조혈간세포이식(allo-HSCT)전후골수혹외주혈단개핵세포중CD4+CD25+Foxp3+조절성T림파세포(이하간칭CD4+CD25+Foxp3+T세포)적비솔、Foxp3 mRNA화Foxp3단백적표체여급성이식물항숙주병(aGVHD)적관계.방법 선택37례행HIA상합이기인조혈간세포이식(allo-HSCT))적환자작위연구대상.채용류식세포술검측환자allo-HSCT전7천(-7 d)、이식당천(0 d)、이식후30천(+30d)、+60 d화+90 d시골수혹외주혈단개핵세포중CD4+CD25+Foxp3+T세포소점적비솔;정량취합매련반응(PCR)검측Foxp3 mRNA상대표체량;단백인적(Western blot)법검측Foxp3적단백표체수평.결과 37례환자존이식후100 d내발생aGVHD 13례(aGVHD조),미발생aGVHD 24례(무aGVHD조).CD4+CD25+Foxp3+T세포적비솔재0 d시최저,여-7、+30、+60、+90d시비교.차이균유통계학의의(P<0.01);여aGVHD조비교,무aGVHD조환자적CD4+CD25+Foxp3+T세포적비솔명현승고,차이유통계학의의(P<0.01).aGVHD조환자이식후Foxp3적표체수평축점승고,단명현저우무aGVHD조,우기재+60화+90 d시적차이유통계학의의(P<0.01).Western blot법검측결과 표명,aGVHD조수자적Foxp3단백표체야명현저우무aGVHD조.결론 CD4+CD25+Foxp3+T세포대방지발생aGVHD구유중요작용,감측환자골수혹외주혈중CD4+CD25+Foxp3+T세포적비솔가이예측aGVHD적발생.
Objective To investigate the expression and significance of CD4+ CD25+ Foxp3+ T Cells, Foxp3 mRNA and Foxp3 protein in the acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Methods Thirty-seven donor grafts and their respective clinical characteristics were evaluated. Flow cytometric analysis was performed to assess the percentage of CD4+ CD25+ Foxp3+ T cells in the recipients before, and 0, 30, 60, and 90 days after allo-HSCT. The relative expression of Foxp3 mRNA was detected by real time reverse transcription-polymerase chain reaction with aGVHD as the internal control gene, and the relative expression of Foxp3 protein by Western blot with α-tubulin as the internal control protein. Results aGVHD occurred in 13 out of 37 patients (aGVHI) group),and the remaining 24 had no aGVHD (no aGVHD group) within 100 days after transplantation. The percentage of CD4+ CD25+ Foxp3+ T cells was the lowest at 0 day of transplantation with the difference being statistically significant difference in comparison to that at other time points (-7, +30, +60,and +90 days) (P<0.01). The percentage of CD4+ CD25+ Foxp3+ T cells in no aGVHD group was significantly higher than in aGVHD group (P <0.01). The expression level of Foxp3 mRNA in aGVHD group was gradually increased after transplantation, but obviously lower than in no aGVHD group, especially at the post-transplantation day 60,and 90,there was significant difference between two group (P<0.01). Western blot revealed that the expression of Foxp3 protein in aGVHD group was significantly lower than in no aGVHD group. Conclusion CD4+ CD25+ Foxp3+ T cells may be of importance in preventing the development of aGVHD. Monitoring of the percentage of CD4+ CD25+ Foxp3+ T cells in bone marrow or peripheral blood can predict the occurrence of aGVHD after transplantation.
