中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
4期
628-631
,共4页
牛莉%杜朝晖%陈超%汪海松%李建国%王焱林
牛莉%杜朝暉%陳超%汪海鬆%李建國%王焱林
우리%두조휘%진초%왕해송%리건국%왕염림
脓毒症%足三里%凝血功能%纤溶功能%电针
膿毒癥%足三裏%凝血功能%纖溶功能%電針
농독증%족삼리%응혈공능%섬용공능%전침
Sepsis%Zusanli%Coagulation%Fibrinolysis%Electro-acupuncturing
目的 观察电针足三里(ST36)对脓毒症大鼠凝血和纤溶功能的影响.方法 取雄性SD大鼠192只,随机分为6组:生理盐水组(SHAM)、脂多糖组(LPS)、电针非经非穴组(Non-EA)、电刺激足三里组(EA)、迷走神经切断组(VGX)、银环蛇毒素组(α-BGT).分别于注射脂多糖前(0时)、注入后2、4、6h4个时点取8只大鼠经腹主动脉取血后处死,离心取血浆用酶联免疫吸附试验(ELISA)法检测肿瘤坏死因子-α(TNF-α)、D-二聚体、纤溶酶原激活物抑制因子-1(PAI-1)、组织型纤溶酶原激活物(t-PA)的水平,用发色底物法检测抗凝血Ⅲ(AT-Ⅲ)的活性.结果 在予以LPS2h后,LPS组(332.71 ±8.23) ng/L和VGX组(449.70±13.74) ng/L和α-BGT组(448.89±16.50) ng/L血浆TNF-α水平较EA组(268.64±5.48) ng/L升高(P＜0.05)；4h时,LPS组(681.27±7.72)ng/L和VGX组(776.29±3.23) ng/L和α-BGT组(769.97±5.50) ng/L与EA组(487.21±5.42) ng/L比较,血浆PAI水平升高(P＜0.05)；2h时,LPS组(22.51±0.47)ng/L和VGX组(27.10 ±0.30)ng/L和α-BGT组(27.01 ±0.57) ng/L与EA组(10.83 ±0.12) ng/L比较,血浆t-PA水平升高；2h时,LPS组(50.94 ±3.33) ng/L和VGX组(73.48 ±4.45) ng/L和α-BGT组(70.10±2.80) ng/L与EA组(29.47±3.58) ng/L比较,血浆D-二聚体水平升高(P＜0.05)；2h时,LPS组(368.76±13.83)和VGX组(244.69 ±4.88)和α-BGT组(241.85 ±5.51)与EA组(426.78 ±3.07)比较,血浆AT-Ⅲ的活性降低(P＜0.05).结论 电针足三里能有效抑制脓毒症大鼠血浆TNF-α的水平,并能减轻炎症反应,对于凝血和纤溶功能紊乱也有明显的改善作用.
目的 觀察電針足三裏(ST36)對膿毒癥大鼠凝血和纖溶功能的影響.方法 取雄性SD大鼠192隻,隨機分為6組:生理鹽水組(SHAM)、脂多糖組(LPS)、電針非經非穴組(Non-EA)、電刺激足三裏組(EA)、迷走神經切斷組(VGX)、銀環蛇毒素組(α-BGT).分彆于註射脂多糖前(0時)、註入後2、4、6h4箇時點取8隻大鼠經腹主動脈取血後處死,離心取血漿用酶聯免疫吸附試驗(ELISA)法檢測腫瘤壞死因子-α(TNF-α)、D-二聚體、纖溶酶原激活物抑製因子-1(PAI-1)、組織型纖溶酶原激活物(t-PA)的水平,用髮色底物法檢測抗凝血Ⅲ(AT-Ⅲ)的活性.結果 在予以LPS2h後,LPS組(332.71 ±8.23) ng/L和VGX組(449.70±13.74) ng/L和α-BGT組(448.89±16.50) ng/L血漿TNF-α水平較EA組(268.64±5.48) ng/L升高(P＜0.05)；4h時,LPS組(681.27±7.72)ng/L和VGX組(776.29±3.23) ng/L和α-BGT組(769.97±5.50) ng/L與EA組(487.21±5.42) ng/L比較,血漿PAI水平升高(P＜0.05)；2h時,LPS組(22.51±0.47)ng/L和VGX組(27.10 ±0.30)ng/L和α-BGT組(27.01 ±0.57) ng/L與EA組(10.83 ±0.12) ng/L比較,血漿t-PA水平升高；2h時,LPS組(50.94 ±3.33) ng/L和VGX組(73.48 ±4.45) ng/L和α-BGT組(70.10±2.80) ng/L與EA組(29.47±3.58) ng/L比較,血漿D-二聚體水平升高(P＜0.05)；2h時,LPS組(368.76±13.83)和VGX組(244.69 ±4.88)和α-BGT組(241.85 ±5.51)與EA組(426.78 ±3.07)比較,血漿AT-Ⅲ的活性降低(P＜0.05).結論 電針足三裏能有效抑製膿毒癥大鼠血漿TNF-α的水平,併能減輕炎癥反應,對于凝血和纖溶功能紊亂也有明顯的改善作用.
목적 관찰전침족삼리(ST36)대농독증대서응혈화섬용공능적영향.방법 취웅성SD대서192지,수궤분위6조:생리염수조(SHAM)、지다당조(LPS)、전침비경비혈조(Non-EA)、전자격족삼리조(EA)、미주신경절단조(VGX)、은배사독소조(α-BGT).분별우주사지다당전(0시)、주입후2、4、6h4개시점취8지대서경복주동맥취혈후처사,리심취혈장용매련면역흡부시험(ELISA)법검측종류배사인자-α(TNF-α)、D-이취체、섬용매원격활물억제인자-1(PAI-1)、조직형섬용매원격활물(t-PA)적수평,용발색저물법검측항응혈Ⅲ(AT-Ⅲ)적활성.결과 재여이LPS2h후,LPS조(332.71 ±8.23) ng/L화VGX조(449.70±13.74) ng/L화α-BGT조(448.89±16.50) ng/L혈장TNF-α수평교EA조(268.64±5.48) ng/L승고(P＜0.05)；4h시,LPS조(681.27±7.72)ng/L화VGX조(776.29±3.23) ng/L화α-BGT조(769.97±5.50) ng/L여EA조(487.21±5.42) ng/L비교,혈장PAI수평승고(P＜0.05)；2h시,LPS조(22.51±0.47)ng/L화VGX조(27.10 ±0.30)ng/L화α-BGT조(27.01 ±0.57) ng/L여EA조(10.83 ±0.12) ng/L비교,혈장t-PA수평승고；2h시,LPS조(50.94 ±3.33) ng/L화VGX조(73.48 ±4.45) ng/L화α-BGT조(70.10±2.80) ng/L여EA조(29.47±3.58) ng/L비교,혈장D-이취체수평승고(P＜0.05)；2h시,LPS조(368.76±13.83)화VGX조(244.69 ±4.88)화α-BGT조(241.85 ±5.51)여EA조(426.78 ±3.07)비교,혈장AT-Ⅲ적활성강저(P＜0.05).결론 전침족삼리능유효억제농독증대서혈장TNF-α적수평,병능감경염증반응,대우응혈화섬용공능문란야유명현적개선작용.
Objective To investigate the effect of the electrical stimulation Zusanli (ST36) on coagulation and fibrinolysis in septic rats.Methods 192 male SD rats were randomly divided into six groups:saline group ( SHAME ),lipopolysaccharide ( LPS ) group,non-acupuncture stimulation group (non-EA),electrical stimulation Zusanli group (EA),vagotomy group (VGX),bungarotoxin group ( α-BGT).Six animals of every four time points [ before injection of LPS (0),2,4 and 6 h after injection]were killed after abdominal aortic blood was collected.The plasma was detected by enzyme linked immunosorbent assay (ELISA) for tumor necrosis factor-α (TNF-α),D-dimer,plasminogen activator inhibitor-Ⅰ (PAI-I) and tissue-type plasminogen activator (t-PA) levels,and the chromogenic substrate assay for antithrombin Ⅲ( AT-Ⅲ ) activity.Results At the 2nd h after injection of LPS,as compared with EA group (268.64 ±5.48) ng/L,the levels of plasma TNF-α in LPS group (332.71 ±8.23) ng/L and VGX group (449.70 ± 13.74) ng/L,and α-BGT group (448.89 ± 16.50) ng/L were significantly increased (P ＜0.05) ; At the 4th h,as compared with EA group (487.21 ±5.42) ng/L,the levels of plasma PAI in LPS group (681.27 ± 7.72) ng/L and VGX group (776.29 ± 3.23 ) ng/L,and e-BGT group (769.97 ± 5.50)ng/L were significantly increased ( P ＜ 0.05) ; At the 2nd h after injection of LPS,as compared with EA group (10.83 ±0.12) ng/L,the levels of plasma t-PA in LPS group (22.51 ± 0.47) ng/L and VGX group (27.10 ± 0.30) ng/L,and α-BGT group (27.01 ± 0.57 ) ng/L were significantly increased (P ＜0.05) ; At the 2nd h after injection of LPS,as compared with EA group (29.47 ± 3.58) ng/L,the levels of plasma D-dimer in LPS group (50.94 ±3.33) ng/L and VGX group (73.48 ±4.45) ng/L,and α-BGT group ( 70.10 ± 2.80 ) ng/L were significantly increased ( P ＜ 0.05 ) ; as compared with EA group (426.78 ±3.07) ng/L,the levels of plasma AT-Ⅲ in LPS group (368.76 ± 13.83) ng/L and VGX group (244.69 ±4.88 ) ng/L,and α-BGT group (241.85 ± 5.51 ) ng/L were significantly increased (P ＜0.05).Conclusion Electrical stimulation Zusanli can not only inhibit plasma levels of TNF-α but also can reduce inflammation,coagulation and fibrinolytic dysfunction in septic rats.
