CAJ | 학술논문

目的分析我国血液制品凝血因子Ⅷ及原料血浆中人B19病毒污染情况及B19病毒的基因型.方法以B19病毒VP1/VP2区引物采用套式PCR法分别检测16批凝血因子Ⅷ成品及10批混合原料血浆中B19病毒的污染情况,并对阳性样品中B19病毒DNA的NS1-VP1u区进行测序和基因型分析.结果 16批凝血因子Ⅷ中B19病毒DNA阳性率为75%(12/16),10批混合原料血浆中B19病毒DNA阳性率为100%(10/10).序列测定结果表明,所有B19阳性样品中的病毒序列高度保守,同源性为98.3%-100%,且均为基因型I型.结论我国凝血因子Ⅷ及原料血浆中B19病毒Ⅰ型污染率高,病毒核苷酸具有较高的保守性.
목적 분석아국혈액제품응혈인자Ⅷ급원료혈장중인B19병독오염정황급B19병독적기인형.방법 이B19병독VP1/VP2구인물채용투식PCR법분별검측16비응혈인자Ⅷ성품급10비혼합원료혈장중B19병독적오염정황,병대양성양품중B19병독DNA적NS1-VP1u구진행측서화기인형분석.결과 16비응혈인자Ⅷ중B19병독DNA양성솔위75%(12/16),10비혼합원료혈장중B19병독DNA양성솔위100%(10/10).서렬측정결과표명,소유B19양성양품중적병독서렬고도보수,동원성위98.3%-100%,차균위기인형I형.결론 아국응혈인자Ⅷ급원료혈장중B19병독Ⅰ형오염솔고,병독핵감산구유교고적보수성.
Objective To study the prevalence and genotype of human parvovirus B19 virus among blood products and plasma pools in China. Methods B19 DNA derived from 16 lots of factor Ⅷ concentrate produced by 4 manufactures and 10 lots of plasma pools were detected by nested PCR. Phylogenetic comparison of the partial B19 sequences obtained from positive samples were performed by direct sequencing. Results Twelve of sixteen lots of factor Ⅷ concentrate and all of ten lots of plasma pools were contaminated by B19 DNA. By comparing the partial B19 sequences,all the isolated viruses were genotype Ⅰ and their nucleotides were high conserved with homology of 98. 3%-100%. Conclusion B19 genotype Ⅰ DNA has been detected in high prevalence in factor Ⅷ concentrate and plasma pools. The genetic diversities were shown to be very low.