CAJ | 학술논문

目的检测肾透明细胞癌患者术前血清中内皮抑素含量,分析其与肿瘤分级、分期的关系.方法 2004年3月至2008年10月接受手术治疗的肾透明细胞癌患者138例,其中男性102例,女性36例,平均年龄63.2岁;T1期73例,T2期39例,T3期20例,T4期6例.40例性别、年龄相应的健康人作为对照组,应用双抗体夹心ELISA法检测其术前血清中内皮抑素水平.结果患者术前血清中内皮抑素含量均值为93.1 μg/L,与健康对照组(78.9 μg/L)差异无统计学意义(P>0.05).T1期患者术前血清内皮抑素水平为80.4 μg/L,低于T2期(107.5 μg/L,P<0.01)、T3期(102.7 μg/L,P<0.05)及T4期(120.7 μg/L,P<0.01),而后三组之间差异无统计学意义(P>0.05).T1期患者与对照组无明显差异(P>0.05).转移组患者术前血清内皮抑素含量为118.4 μg/L,高于未转移组的89.5 μg/L(P<0.05);单纯淋巴结转移组及远处转移组患者之间差异无统计学意义.G3-4级患者血清内皮抑素水平为111.8 μg/L,高于G1级(80.4 μg/L)和G2级(86.2 μg/L)(P<0.01).结论肾透明细胞癌患者术前血清内皮抑素含量与肿瘤的高分级、高分期相关,可能有助于患者预后及肿瘤分化程度判断.
목적 검측신투명세포암환자술전혈청중내피억소함량,분석기여종류분급、분기적관계.방법 2004년3월지2008년10월접수수술치료적신투명세포암환자138례,기중남성102례,녀성36례,평균년령63.2세;T1기73례,T2기39례,T3기20례,T4기6례.40례성별、년령상응적건강인작위대조조,응용쌍항체협심ELISA법검측기술전혈청중내피억소수평.결과 환자술전혈청중내피억소함량균치위93.1 μg/L,여건강대조조(78.9 μg/L)차이무통계학의의(P>0.05).T1기환자술전혈청내피억소수평위80.4 μg/L,저우T2기(107.5 μg/L,P<0.01)、T3기(102.7 μg/L,P<0.05)급T4기(120.7 μg/L,P<0.01),이후삼조지간차이무통계학의의(P>0.05).T1기환자여대조조무명현차이(P>0.05).전이조환자술전혈청내피억소함량위118.4 μg/L,고우미전이조적89.5 μg/L(P<0.05);단순림파결전이조급원처전이조환자지간차이무통계학의의.G3-4급환자혈청내피억소수평위111.8 μg/L,고우G1급(80.4 μg/L)화G2급(86.2 μg/L)(P<0.01).결론 신투명세포암환자술전혈청내피억소함량여종류적고분급、고분기상관,가능유조우환자예후급종류분화정도판단.
Objective To assess serum levels of endogenous endostatin in patients with clear cell renal cell carcinoma (CCRCC) and to determine the relationship of these levels to tumor stage, grade. Methods From March 2004 to October 2008, preoperative serum were obtained from 138 consecutive patients with CCRCC (73 patients in T1, 39 patients in T2, 20 patients in T3, and 6 patients in T4) and 40 healthy controls. Serum levels of endostatin were measured by sandwich-ELISA. Associations between circulating endostatin levels and clinicopathologic factors and clinical outcome were determined.ResultsEndostatin levels did not differ significantly between the patients with CCRCC(93.1 μg/L)and healthy controls (78.9 μg/L, P>0.05). Serum levels of endostatin were significantly higher in the T2-4 CCRCC patients (107.2 μg/L) than those of the T1 patients (80.4 μg/L, P<0.01). No significant difference was found in the endostatin levels among the T2-4 patients, or between healthy controls and the T1 patients. The serum endostatin concentration was significantly higher in the metastasis group (118.4 μg/L) than in the no metastasis group (89.5 μg/L, P<0.05), but there was no significant difference between patients with distant metastasis group (122.0 μg/L)and lymph nodes metastasis (110.0 μg/L, P>0.05). Patients with G3-4 tumors had significantly higher endostatin levels (111.8 μg/L) than those of patients with G1 (80.4 μg/L) and G2 tumors (86.2 μg/L, P<0.01), but endostatin levels did not differ significantly between the two groups (P>0.05). Conclusion Preoperative serum levels of endostatin elevated in patients with CCRCC and associated with higher stage and grade.