CAJ | 학술논문

细胞质型果糖-1,6-二磷酸基因ATG上游1 195bp侧翼序列可调控GUS基因在水稻(Oryza sativa L.)中特异性表达,因此该片段包含有使报告基因在叶肉细胞中特异性表达的所有顺式元件.为了研究其调控特异表达的顺式元件,对启动子5′端进行了一系列的缺失,得到4种与GUS基因融合的植物表达载体,通过基因枪法转入水稻.结果表明,自启动子5′端-1 195 bp缺失至-1 102 bp时,GUS基因由叶肉细胞特异性表达变为组成型表达,且表达活性有所提高,推测在该区段中存在调控叶肉细胞特异性表达的顺式元件.进一步缺失仍然保持组成性表达的模式,即在转化株的根、茎和叶中的所有细胞中均有表达,同时启动子活性有所提高.这一结果暗示该启动子具有很大的应用潜力.
세포질형과당-1,6-이린산기인ATG상유1 195bp측익서렬가조공GUS기인재수도(Oryza sativa L.)중특이성표체,인차해편단포함유사보고기인재협육세포중특이성표체적소유순식원건.위료연구기조공특이표체적순식원건,대계동자5′단진행료일계렬적결실,득도4충여GUS기인융합적식물표체재체,통과기인창법전입수도.결과표명,자계동자5′단-1 195 bp결실지-1 102 bp시,GUS기인유협육세포특이성표체변위조성형표체,차표체활성유소제고,추측재해구단중존재조공협육세포특이성표체적순식원건.진일보결실잉연보지조성성표체적모식,즉재전화주적근、경화협중적소유세포중균유표체,동시계동자활성유소제고.저일결과암시해계동자구유흔대적응용잠력.
The 1 195 bp 5′ flanking region of rice (Oryza sativa L.) cytosolic fructose-1, 6-bisphosphatase (cyFBPase) can direct tissue, cell specific expression in transgenic rice. In order to identify sequence elements responsible for the regulation of mesophyll-specific expression, the 5′ flanking regions of -1 195 bp, -1 102 bp, -768 bp, and -644 bp upstream of the translation initiation ATG codon were fused to the reporter gene encoding β-glucuronidase (GUS) and transferred to rice via particle bombardment. Analysis of the 5′ promoter deletions identified that a 93 bp fragment between -1 195 bp and -1 102 bp is essential for directing mesophyll specific expression. High constitutive expression of GUS reporter gene was found in the -768 deletion lines and another two deletion series. These results indicate the great potential utility of the promoter in rice biotechnology.