CAJ | 학술논문

背景:木芙蓉叶是我国著名伤科魏指薪教授的在临床应用于消非特异性炎症的非常有效的药物,已经有近60年历史,为了阐明该药的作用机制,以缺血再灌注损伤的模型进行研究,来说明其抗炎的作用机制.目的:观察木芙蓉叶有效组分(MFR)对大鼠肾缺血再灌注损伤中的保护作用,探讨MFR的抗炎作用机制.设计:以实验动物为研究对象的随机对照实验研究.单位:一所市级伤骨科研究所.材料:实验于2002-06/2003-06在国家中医药管理局重点实验室(上海市伤骨科研究所内实施完成),选取雄性Wistar大鼠55只.方法:采用大鼠肾缺血再灌注模型,木芙蓉叶有效组分灌胃,检测血清尿素氮、肌酐,并检测白细胞介素-1(IL-1)的含量.主要观察指标:①血尿素氮及肌酐的变化.②MFR对血清IL-1含量的影响.结果:经MFR治疗后,能明显改善大鼠缺血再灌注后肾功能的变化.假手术组大鼠血清尿素氮为(6.72±1.30)mmol/L,血清肌酐为(38 40±6.23)μmol/L.缺血1 h并再灌注24 h后,对照组大鼠尿素氮为(60.72±4.64)mmol/L,而MFR治疗组尿素氮为(47.34±8.32)mmol/L,两组之间的差异有显著性意义(t=2.562,P＜0.05);治疗组和对照组的血清肌酐分别为(347.95±95)μmol/L和(518.20±41.15)μmol/L,两者之间差异具有显著性意义(t=3.69,P＜0.01).在IL-1的作用方面:肾缺血1 h,再灌注1 h后,MFR治疗组IL-1为(122.79±27.56)ng/L,对照组为(180 28±33.15)ng/L,治疗组IL-1水平明显低于对照组(t=2.98,P＜0.05);缺血1 h,再灌注3 h后,治疗组和对照组IL-1含量分别为(15 58±8.59)ng/L和(34.13±10.02)ng/L,两组之间差异具有显著性意义(t=3.14,P＜0.05).结论:木芙蓉叶有效组分对肾缺血再灌注损伤有保护作用,其原因可能与抑制IL-1等的生成有关.
배경:목부용협시아국저명상과위지신교수적재림상응용우소비특이성염증적비상유효적약물,이경유근60년역사,위료천명해약적작용궤제,이결혈재관주손상적모형진행연구,래설명기항염적작용궤제.목적:관찰목부용협유효조분(MFR)대대서신결혈재관주손상중적보호작용,탐토MFR적항염작용궤제.설계:이실험동물위연구대상적수궤대조실험연구.단위:일소시급상골과연구소.재료:실험우2002-06/2003-06재국가중의약관리국중점실험실(상해시상골과연구소내실시완성),선취웅성Wistar대서55지.방법:채용대서신결혈재관주모형,목부용협유효조분관위,검측혈청뇨소담、기항,병검측백세포개소-1(IL-1)적함량.주요관찰지표:①혈뇨소담급기항적변화.②MFR대혈청IL-1함량적영향.결과:경MFR치료후,능명현개선대서결혈재관주후신공능적변화.가수술조대서혈청뇨소담위(6.72±1.30)mmol/L,혈청기항위(38 40±6.23)μmol/L.결혈1 h병재관주24 h후,대조조대서뇨소담위(60.72±4.64)mmol/L,이MFR치료조뇨소담위(47.34±8.32)mmol/L,량조지간적차이유현저성의의(t=2.562,P＜0.05);치료조화대조조적혈청기항분별위(347.95±95)μmol/L화(518.20±41.15)μmol/L,량자지간차이구유현저성의의(t=3.69,P＜0.01).재IL-1적작용방면:신결혈1 h,재관주1 h후,MFR치료조IL-1위(122.79±27.56)ng/L,대조조위(180 28±33.15)ng/L,치료조IL-1수평명현저우대조조(t=2.98,P＜0.05);결혈1 h,재관주3 h후,치료조화대조조IL-1함량분별위(15 58±8.59)ng/L화(34.13±10.02)ng/L,량조지간차이구유현저성의의(t=3.14,P＜0.05).결론:목부용협유효조분대신결혈재관주손상유보호작용,기원인가능여억제IL-1등적생성유관.
BACKGROUND: Hibiscus Mutabilis L. Is a very effective Chinese traditional herb for non-specific inflammation in the clinic adopted by Professor Wei Zhixin, the eminent expert in Traumatology Department in China, lasting for near 60 years. In order to explain its pharmaceutical mechanism, the research of Hibiscus Mutabilis L. Was carried on in the model of ischemic reperfusion injury to explain its mechanism on anti-inflammation.OBJECTIVE: To observe the protection of the effective fraction of Hibiscus Mutabilis L. (MFR) on renal ischemic reperfusion injury in rats to probe into the mechanism of MFR on anti-inflammation.DESIGN: Randomized and controlled study on experimental animal.SETTING: A Municipal Institute of Traumatology and Orthopedic Department.MATERIALS: The experiment was performed in the Key Experiment Room (Institute of Traumatology and Orthopedic Department, Shanghai) of State Administration of Traditional Chinese Medicine, in which, 55 male Wistar rats were employed.METHODS: The rat model of renal ischemic reperfusion was adopted and MFR was applied for gastric perfusion to determine serum blood urea nitrogen(BUN) and serum creatinine(Scr) and the level of interleukin-1 (IL-1).MAIN OUTCOME MEASURES: ① Changes in serum BUN and Scr; ②Effect of MFR on level of serum IL-1.RESULTS: After MFR treatment, the renal function was improved remarkably after ischemic reperfusion in rats. In sham-operation group, serun BUN was (6.72 ± 1.30) mmol/L and serum Scr was (38.40 ± 6.23) μmol/L. In 24hours of reperfusion after one-hour ischemia, BUN was(60. 72±4.64)mmol/L in the control and(47.34 ± 8.32) mmol/L in the MFR treatment group, and the significant difference presented between two groups(t=2.562, P ＜ 0.05) .Serum Scr was(347.95±95) μmol/L and(518.20 ± 41.15) μmol/L in the treatment group and the control respectively, indicating significant difference ( t = 3.69, P ＜ 0.01 ) . Concerning to the effect on IL-1, in 1 hour of reperfusion after 1 hour renal ischemia,IL-1 was( 122.79 ±27.56) ng/L in the MER treatment group,(180. 28 ±33. 15) ng/L in the control group, indicating that IL-1 level in the treatment group was remarkably superior to that in the control group(t = 2.98, P ＜ 0.05). In 3 hours of reperfusion after 1 hour ischemia, level of IL-1 in the treatment group and control group was(15.58±8.59) ng/L and (34. 13±± 10. 02) ng/L respectively, indicating significant difference( t = 3.14, P＜ 0.05).CONCLUSION: MFR provides protection on renal ischemic reperfusion injury, which probably is related to its inhibition on IL-1 formation.