神经科学通报（英文版）
神經科學通報（英文版）
신경과학통보（영문판）
NEUROSCIENCE BULLETIN
2007年
2期
113-118
,共6页
电刺激%视顶盖%牛蛙
電刺激%視頂蓋%牛蛙
전자격%시정개%우와
electric stimulation%optic tectum%Rana catesbeiana
目的 已有许多研究报告了青蛙的前视盖对视顶盖起抑制作用,但关于此神经活动的特性尚不清楚.本研究探讨了这种复杂的神经活动的机理.方法 用细胞内记录方法,通过电刺激前视盖的神经细胞核来记录视顶盖细胞的神经活动.结果前视盖的电刺激在同侧视顶盖主要唤起了两种神经反应:一种是兴奋性(excitatory postsynaptic potential,EPSP)和抑制性突触后电位(an inhibitory postsynaptic potential,IPSP)同时出现,另一种是单纯的IPSP,后者在本记录中占主导地位.另外我们也记录到了某些投射到前视盖的视盖投射细胞的神经电位.它揭示了视顶盖和前视盖之间存在着交叉性的相互作用.短潜时的EPSP可能是通过单突触进行传导的,而大多数的IPSP是通过多突触方式进行神经信息传递的.几乎98%被记录的视盖细胞对前视盖的刺激显示出了抑制性反应.结论 前视盖的神经细胞对视顶盖的神经活动发挥了强烈的抑制性作用.
目的 已有許多研究報告瞭青蛙的前視蓋對視頂蓋起抑製作用,但關于此神經活動的特性尚不清楚.本研究探討瞭這種複雜的神經活動的機理.方法 用細胞內記錄方法,通過電刺激前視蓋的神經細胞覈來記錄視頂蓋細胞的神經活動.結果前視蓋的電刺激在同側視頂蓋主要喚起瞭兩種神經反應:一種是興奮性(excitatory postsynaptic potential,EPSP)和抑製性突觸後電位(an inhibitory postsynaptic potential,IPSP)同時齣現,另一種是單純的IPSP,後者在本記錄中佔主導地位.另外我們也記錄到瞭某些投射到前視蓋的視蓋投射細胞的神經電位.它揭示瞭視頂蓋和前視蓋之間存在著交扠性的相互作用.短潛時的EPSP可能是通過單突觸進行傳導的,而大多數的IPSP是通過多突觸方式進行神經信息傳遞的.幾乎98%被記錄的視蓋細胞對前視蓋的刺激顯示齣瞭抑製性反應.結論 前視蓋的神經細胞對視頂蓋的神經活動髮揮瞭彊烈的抑製性作用.
목적 이유허다연구보고료청와적전시개대시정개기억제작용,단관우차신경활동적특성상불청초.본연구탐토료저충복잡적신경활동적궤리.방법 용세포내기록방법,통과전자격전시개적신경세포핵래기록시정개세포적신경활동.결과전시개적전자격재동측시정개주요환기료량충신경반응:일충시흥강성(excitatory postsynaptic potential,EPSP)화억제성돌촉후전위(an inhibitory postsynaptic potential,IPSP)동시출현,령일충시단순적IPSP,후자재본기록중점주도지위.령외아문야기록도료모사투사도전시개적시개투사세포적신경전위.타게시료시정개화전시개지간존재착교차성적상호작용.단잠시적EPSP가능시통과단돌촉진행전도적,이대다수적IPSP시통과다돌촉방식진행신경신식전체적.궤호98%피기록적시개세포대전시개적자격현시출료억제성반응.결론 전시개적신경세포대시정개적신경활동발휘료강렬적억제성작용.
Objective A few investigations have been reported about pretectal suppressive influences on the optic tectum of frog, but characteristics of tectal activity to pretectal input are left unknown. We made intracellular recordings to demonstrate the unexpected complexity in synaptic mechanisms involved in the suppressive influences of pretecal stimulation on the tectal cells. Methods In the present study, we investigated the neuronal activity evoked by pretectal (Lpd/P) nuclei stimulation using intracellular recording technique. Results The pretectal stimulation mainly elicited two types of responses in the ipsilateral tectum: an excitatory postsynaptic potential (EPSP) followed by an inhibitory postsynaptic potential (IPSP) and a pure IPSP. The latter predominated in the tectal cells responding to pretectal stimulation. In a few cells, biphasic hyperpolarization appeared under stronger stimulus intensities. The spikes of tecto-pretectal projecting cells elicited by antidromical stimulation were recorded in the ipsilateral tectum, which revealed reciprocal connections between the tectum and particular pretectal nuclei. The synaptic natures underlying pretecto-tectal information transformation have also been demonstrated. EPSPs with short latencies were concluded to be monosynaptic. Most IPSPs were generated through polysynaptic paths, but monosynaptic IPSPs were also recorded in the tectum. Nearly 98% of impaled tectal cells (except for antidromically projecting cells) showed inhibitory responses to pretectal stimulation. Conclusion The results provide strong evidence that pretectal cells broadly inhibit tectal neurons as that has suggested by behavioral and extracellular recording studies.
