CAJ | 학술논문

本研究旨在探讨Eph/Ephrin-B1信号对急性分离的鸡胚睫状神经节(ciliary ganglion,CG)神经元上α3-尼古丁乙酰胆碱受体(nicotinic acetylcholine receptors,α3-nAChRs)和α7-尼古丁乙酰胆碱受体(nicotinic acetylcholine receptors,α7-nAChRs)电流的影响及可能机制.用膜片钳技术在急性分离的CG神经元上分别记录α3-nAChRs和α7-nAChRs全细胞电流.为检测Ephrin-B1对细胞nAChRs电流的影响,细胞被随机分为:对照组、Ephrin-B1Fc处理组(用Ephrin-B1与IgG重组后形成的Ephrin-B1Fc刺激细胞)、IgG处理组(用与Ephrin-B1Fc处理组重组所需的相同浓度的IgG刺激细胞)和Ephrin-B1处理组(用与Ephrin-B1Fc处理组重组所需的相同浓度的Ephrin-B1刺激细胞).为探讨Ephrin-B1调节细胞nAChRs电流的机制,分别用Src信号抑制剂PP2和丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号抑制剂PD98095预处理细胞后,再用Ephrin-B1Fc处理细胞,观察α3-nAChRs和α7-nAChRs电流的变化,细胞被随机分为:对照组、Ephrin-B1Fc处理组、PP2或PD98095(PP2/PD)处理组、Ephrin-B1Fc+PP2或PD98095处理组.结果显示:对照组、IgG处理组和Ephrin-B1处理组之间α3-nAChRs和α2-nAChRs电流无显著差异,而Ephrin-B1Fc处理组可显著抑制α3-nAChRs和α7-nAChRs电流,与对照组比较有显著差异(P=0.002、P=0.003);此外,PP2可部分恢复被Ephrin-B1Fc所抑制的α7-nAChRs电流,PD98095则可部分恢复被Ephrin-B1Fc所抑制的α3-nAChRs电流.以上结果提示,Eph/Ephrin-B1信号可能分别通过MAPK和Src信号途径抑制急性分离的CG神经元上α3-nAChRs和α7-nAChRs的电流,表明Eph/Ephrin-B1可能参与交感神经系统功能的调控.
본연구지재탐토Eph/Ephrin-B1신호대급성분리적계배첩상신경절(ciliary ganglion,CG)신경원상α3-니고정을선담감수체(nicotinic acetylcholine receptors,α3-nAChRs)화α7-니고정을선담감수체(nicotinic acetylcholine receptors,α7-nAChRs)전류적영향급가능궤제.용막편겸기술재급성분리적CG신경원상분별기록α3-nAChRs화α7-nAChRs전세포전류.위검측Ephrin-B1대세포nAChRs전류적영향,세포피수궤분위:대조조、Ephrin-B1Fc처리조(용Ephrin-B1여IgG중조후형성적Ephrin-B1Fc자격세포)、IgG처리조(용여Ephrin-B1Fc처리조중조소수적상동농도적IgG자격세포)화Ephrin-B1처리조(용여Ephrin-B1Fc처리조중조소수적상동농도적Ephrin-B1자격세포).위탐토Ephrin-B1조절세포nAChRs전류적궤제,분별용Src신호억제제PP2화사렬원활화단백격매(mitogen-activated protein kinase,MAPK)신호억제제PD98095예처리세포후,재용Ephrin-B1Fc처리세포,관찰α3-nAChRs화α7-nAChRs전류적변화,세포피수궤분위:대조조、Ephrin-B1Fc처리조、PP2혹PD98095(PP2/PD)처리조、Ephrin-B1Fc+PP2혹PD98095처리조.결과현시:대조조、IgG처리조화Ephrin-B1처리조지간α3-nAChRs화α2-nAChRs전류무현저차이,이Ephrin-B1Fc처리조가현저억제α3-nAChRs화α7-nAChRs전류,여대조조비교유현저차이(P=0.002、P=0.003);차외,PP2가부분회복피Ephrin-B1Fc소억제적α7-nAChRs전류,PD98095칙가부분회복피Ephrin-B1Fc소억제적α3-nAChRs전류.이상결과제시,Eph/Ephrin-B1신호가능분별통과MAPK화Src신호도경억제급성분리적CG신경원상α3-nAChRs화α7-nAChRs적전류,표명Eph/Ephrin-B1가능삼여교감신경계통공능적조공.