CAJ | 학술논문

目的:研究ATF-2通过bcl-2影响髁突软骨生长发育的机制.方法:应用体外培养原代小鼠髁突软骨细胞,原代细胞基因转染,蛋白印迹杂交等技术,阐明ATF-2调控bcl-2表达的机制.结果:外源性的ATF-2在髁突软骨细胞中,与bcl-2的调控子中CRE结合,增强bcl-2调控子的活性;当CRE位点的序列改变,ATF-2不能在髁突软骨细胞中对bcl-2调控子的活性产生影响.结论:ATF-2在软骨细胞中,通过影响有凋亡抑制作用的bcl-2的活性和表达,参与下颌骨髁突软骨的生长发育.
목적:연구ATF-2통과bcl-2영향과돌연골생장발육적궤제.방법:응용체외배양원대소서과돌연골세포,원대세포기인전염,단백인적잡교등기술,천명ATF-2조공bcl-2표체적궤제.결과:외원성적ATF-2재과돌연골세포중,여bcl-2적조공자중CRE결합,증강bcl-2조공자적활성;당CRE위점적서렬개변,ATF-2불능재과돌연골세포중대bcl-2조공자적활성산생영향.결론:ATF-2재연골세포중,통과영향유조망억제작용적bcl-2적활성화표체,삼여하합골과돌연골적생장발육.
Objective: To study the role of activating transcription factor 2 (ATF-2) in the growth of mandibular condyle cartilage. Methods: Primary chondrocytes of condyle were cultured. Expression plasmid of ATF-2 and plasmid bcl-2 promoter were transfected into chondrocytes. Luciferase assay and Western blot were used. Results: The absence of ATF-2 in mandibular condyle chondrocytes resulted in a decline in bcl-2 promoter activity, reduction in bcl-2 protein level. Conclusion: The results strongly imply that ATF-2 is required for adequate bcl-2 expression, and play a significant role in controlling growth plate chondrocyte progression.