CAJ | 학술논문

在实验室条件下,采用菌丝生长速率法测定了几丁寡糖对烟草赤星病菌的抑制活性.继而在温室盆栽和人工接种条件下,分别研究了几丁寡糖、几丁寡糖+木霉+几丁质等5种方法处理对烟草赤星病的防治效果.同时分别系统测定了烟草植株施用几丁寡糖及人工接种赤星病菌后10 d内SOD、POD、PP0和几丁质酶等4种防御酶的活性.结果表明.几丁寡糖对烟草赤星病菌的菌丝生长有一定抑制作用.单独施加几丁寡糖的处理对烟草赤星病的防治效果为53.60%.其次为几丁寡糖+木霉处理.防治效果为38.28%.几丁寡糖处理后烟草体内的PPO、SOD、POD和几丁质酶的活性显著提高.以上结果说明.几丁寡糖对烟草赤星病有显著的控制效应.主要是通过诱导、提高烟草植株有关防御酶的活性从而增强烟草植株对赤星病的抗病性.
재실험실조건하,채용균사생장속솔법측정료궤정과당대연초적성병균적억제활성.계이재온실분재화인공접충조건하,분별연구료궤정과당、궤정과당+목매+궤정질등5충방법처리대연초적성병적방치효과.동시분별계통측정료연초식주시용궤정과당급인공접충적성병균후10 d내SOD、POD、PP0화궤정질매등4충방어매적활성.결과표명.궤정과당대연초적성병균적균사생장유일정억제작용.단독시가궤정과당적처리대연초적성병적방치효과위53.60%.기차위궤정과당+목매처리.방치효과위38.28%.궤정과당처리후연초체내적PPO、SOD、POD화궤정질매적활성현저제고.이상결과설명.궤정과당대연초적성병유현저적공제효응.주요시통과유도、제고연초식주유관방어매적활성종이증강연초식주대적성병적항병성.
Hyphal growth rate method was used to determine the inhibitory activities of chitooligosancharide (COS) against the pathogenic fungus of tobacco brown spot,A Iternaria alternate (Fries) Keissler,in the laboratory,and tobacco plants were applied with COS and its combinations like COS + Trichoderma + chitin with 5 treatments to control the disease under conditions of pot culture and inoculation in the greenhouse. Activities of 4 defensive enzymes,superoxide dismutase (SOD),polyphenol oxidase (PPO),peroxidase(POD) and chitinase,in tobacco plants were determined systematically at the same time within 10 days after application of COS and inoculation with the pathogenic fungus. The results showed that the COS restrained the hyphal growth of A. alternata on PDA in vitro. In the greenhouse experiment,the single COS treatment had a control efficacy of 53.60% against the disease,followed by the COS + Trichoderma treatment(38.28%). The COS treatments improved evidently the activities of SOD,PPO,POD and chitinase in the tobacco plants. All these results suggest that the significant control efficacy of the COS against the tobacco brown spot be mainly due to induction and improvement of the activities of defensive enzymes in the tobacco plants,which enhance resistance of the tobacco plants against the brown spot.