南方医科大学学报
南方醫科大學學報
남방의과대학학보
JOURNAL OF SOUTHERN MEDICAL UNIVERSITY
2009年
10期
1987-1989,1993
,共4页
细胞疗法%毛囊干细胞%毛乳头细胞%毛发再生
細胞療法%毛囊榦細胞%毛乳頭細胞%毛髮再生
세포요법%모낭간세포%모유두세포%모발재생
cell therapy%hair follicle stem cells%dermal papilla cells%hair regeneration
目的 构建一个可靠有效的移植毛囊细胞诱导毛发发育的模型,以治疗脱发.方法 取自愿捐献的成人头皮标本,联用显微分离与免疫磁珠法获得人毛囊干细胞;消化法获得毛乳头细胞.培养后混合植入裸鼠皮下,观察毛囊形成情况.结果 在裸鼠的皮肤切片中可以看到较为完整的毛囊结构形成.结论 毛囊细胞移植法可以在体内诱导出毛囊样结构,为将来治疗脱发奠定了基础.
目的 構建一箇可靠有效的移植毛囊細胞誘導毛髮髮育的模型,以治療脫髮.方法 取自願捐獻的成人頭皮標本,聯用顯微分離與免疫磁珠法穫得人毛囊榦細胞;消化法穫得毛乳頭細胞.培養後混閤植入裸鼠皮下,觀察毛囊形成情況.結果 在裸鼠的皮膚切片中可以看到較為完整的毛囊結構形成.結論 毛囊細胞移植法可以在體內誘導齣毛囊樣結構,為將來治療脫髮奠定瞭基礎.
목적 구건일개가고유효적이식모낭세포유도모발발육적모형,이치료탈발.방법 취자원연헌적성인두피표본,련용현미분리여면역자주법획득인모낭간세포;소화법획득모유두세포.배양후혼합식입라서피하,관찰모낭형성정황.결과 재라서적피부절편중가이간도교위완정적모낭결구형성.결론 모낭세포이식법가이재체내유도출모낭양결구,위장래치료탈발전정료기출.
Objective To establish a convenient and reliable method for inducing hair regeneration by follicular cell implantation for the treatment of alopecia. Methods The human hair follicle stem cells were separated and purified by micromanipulation and magnetic cell sorting, and human scalp dermal papilla cells were isolated by enzyme digestion. The two cells were mixed and implanted subcutaneously in nude mice to observe the regeneration of the hair follicles. Results Formation of intact hair follicle-like structures was observed in the skin sections of the recipient nude mice. Conclusion Follicular cell implantation can induce hair follicle-like structures in nude mice, which provides a means for efficient hair regeneration for treatment of hair loss.
